Variability of breeding system, caryopsis microstructure and germination in annual and perennial species of the genus Brachypodium P. Beauv.

Kosina, Romuald; Tomaszewska, Paulina

doi:10.1007/s10722-015-0297-4

Cited by 5 publications

(5 citation statements)

References 72 publications

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“…Pollen variability (Dzyuba et al 2006;Karabournioti et al 2007;Costa et al 2016) can be evaluated by assessing pollen viability, morphology and pollen-stigma compatibility reactions, thus obtaining crucial information about the plant breeding systems (Kosina and Tomaszewska 2015). Pollen size (De Storme et al 2013) and number of poruses (Kihara 1982;Kalinowski et al 2001Kalinowski et al , 2005 are other important biological parameters for assessing the ploidy of plants, especially in newly formed hybrid units.…”

Section: Discussionmentioning

confidence: 99%

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Variability in the quality of pollen grains in oat amphiploids and their parental species

Tomaszewska

Kosina

2022

Braz. J. Bot

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The pollen analysis has become an important technique to evaluate the use of selectively breeding crops, including the three most important grasses to human nutrition. This study aimed to evaluate the reproductive potential of oat species and their hybrid progeny (amphiploids) during three consecutive vegetation seasons. Correlation and regression analyses were used to describe the morphotypes and viability of pollen grains, while numerical taxonomy methods were applied to analyse the relationships between taxa. The results indicated a difference in the size of pollen grains between the growing seasons, but a stable association between the taxa. The viability of pollen grains showed no correlation with pollen length. In the ordination space, amphiploids and parental species were well discriminated. Amphiploids and parental species were characterised by a positive correlation between the pollen size and the level of ploidy; however, along the respective regression line, the amphiploids were located among species with a high level of ploidy. Developmental anomalies of pollen grains were more frequent in amphiploids, with few pollen grains being chromosomally imbalanced, and the formation of micrograins was the most common event. Multiporate pollens being of multiple-spindle mother cells origin can be equivalents of four monoporate microspores. A strong correlation was observed between the frequencies of multiporate grains and micropollens. In the ordination space, monoporate types (species) were discriminated from multiporate types (amphiploids). High viability of pollen in amphiploids proved their genomic/chromosomal stabilisation across many generations of reproduction.

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Section: Discussionmentioning

confidence: 99%

“…montanum (Małecka 2008). Further, natural mutations or transgressive segregation may occur in the pollen sac of the genus Avena, broadening the old by new variability (Kosina 2015).…”

Section: Discussionmentioning

confidence: 99%

Variability in the quality of pollen grains in oat amphiploids and their parental species

Tomaszewska

Kosina

2022

Braz. J. Bot

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“…al. 2007; Costa et al 2016) can be evaluated by measuring pollen viability, assessing its morphology, and studying pollen-stigma compatibility reactions, thus obtaining important information on the plant breeding systems (Kosina and Tomaszewska 2015). Other biological parameters, such as pollen size (De Storme et al 2013) and number of poruses (Kihara 1982; Kalinowski et al 2001, 2005), are important for assessing the ploidy of plants, in particular of newly formed hybrid units.…”

Section: Discussionmentioning

confidence: 99%

Variability of pollen grains quality in oat amphiploids and their parental species

Tomaszewska

Kosina

2021

Preprint

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The reproductive potential of oat species and their hybrid progeny (amphiploids) was evaluated during three vegetation seasons. Morphotypes and viability of pollen grains were described by means of correlations and regression, while relationships between taxa were analysed with the use of numerical taxonomy methods. Size of pollen grains varied between the growing seasons, but the relations between the taxa appeared to be stable. Viability of pollen grains was environmentally modified and showed no correlation with pollen length. In an ordination space, amphiploids were discriminated from parental species. In both group of plants, a positive correlation between the pollen size and the level of ploidy was maintained; however, along a regression line, amphiploids were located among species with a high level of ploidy and were extreme units deviating from the regression line. Developmental anomalies of pollen grains had a low frequency, with the formation of micrograins being the most common event. Such a pattern of development can prove that some pollen grains were chromosomally unbalanced. Anomalous morphotypes of pollen were more common in hybrid types than in species, including pollens with many poruses, which were found only in amphiploids. Frequencies of multiporate grains and micropollens were strongly correlated. In an ordination space, monoporate types (species) were discriminated from multiporate types (amphiploids). In general, the high level of pollen viability in amphiploids can prove their genomic stabilisation through many generations of their reproduction.

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“…Barrero et al [ 29 ] provided an anatomical description of Brachypodium caryopsis and also studied grain dormancy. Later, studies on the global histone modifications and DNA methylation changes during embryonic and postembryonic growth in Brachypodium [ 30 , 31 ] and on the breeding system, caryopsis structure, and germination [ 32 ] in annual and perennial Brachypodium species were performed.…”

Section: Discussionmentioning

confidence: 99%

Germination and the Early Stages of Seedling Development in Brachypodium distachyon

Wolny

Betekhtin

Rojek

et al. 2018

IJMS

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Successful germination and seedling development are crucial steps in the growth of a new plant. In this study, we investigated the course of the cell cycle during germination in relation to grain hydration in the model grass Brachypodium distachyon (Brachypodium) for the first time. Flow cytometry was performed to monitor the cell cycle progression during germination and to estimate DNA content in embryo tissues. The analyses of whole zygotic embryos revealed that the relative DNA content was 2C, 4C, 8C, and 16C. Endoreplicated nuclei were detected in the scutellum and coleorhiza cells, whereas the rest of the embryo tissues only had nuclei with a 2C and 4C DNA content. This study was accompanied by a spatiotemporal profile analysis of the DNA synthetic activity in the organs of Brachypodium embryos during germination using EdU labelling. Upon imbibition, nuclear DNA replication was initiated in the radicle within 11 h and subsequently spread towards the plumule. The first EdU-labelled prophases were observed after 14 h of imbibition. Analysis of selected genes that are involved in the regulation of the cell cycle, such as those encoding cyclin-dependent kinases and cyclins, demonstrated an increase in their expression profiles.

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Variability of breeding system, caryopsis microstructure and germination in annual and perennial species of the genus Brachypodium P. Beauv.

Cited by 5 publications

References 72 publications

Variability in the quality of pollen grains in oat amphiploids and their parental species

Variability in the quality of pollen grains in oat amphiploids and their parental species

Variability of pollen grains quality in oat amphiploids and their parental species

Germination and the Early Stages of Seedling Development in Brachypodium distachyon

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