Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents

Abstract

Abstract. Samiyarsih S, Naipospos N, Palupi D. 2019. Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents. Biodiversitas 20: 2986-2993. Catharanthus roseus (L.) is an important medicinal plant of the family Apocynaceae used to treat many of the diseases. C. roseus also possess good antioxidant potential. The purpose of this research was 1) to determine the variability of C. roseus based on the morphological, anatomical characters and chlorophyll contents, 2) to know the differences in morphological, anatomical characteristics and chlorophyll contents of C. roseus found in Banyumas Regency. The research used a survey method, with a purposive sampling technique. Preparation of leaf for anatomical analysis was based on paraffin method. The results showed that C. roseus in Banyumas Regency were grouped into eight varieties. The morphological characters observed consisted of 21 characters, 8 quantitative characters, and 13 qualitative characters. Most of the quantitative morphological and anatomical characters were significantly diverse (p <0.05) except for petiole length, corolla number, stomata width and number of trichomata. The eight varieties of C. roseus have different chlorophyll a, chlorophyll b, and total chlorophyll contents but their anthocyanin contents were not significantly different.

Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS

“…All cultivar have different significant characters in anatomical and physiological aspects. The detection of different cultivars based on the color of the petals and eyes of the flower and the middle shade (Samiyarsih et al 2019). The eight cultivars of C. roseus have the color of corolla (petals), which is White with white eye with yellow center, Milky white with red radiating eye with small red center, Whitish pink with pink radiating eye with yellow center, Pinkish red with pink radiating eye with yellow center, Dark pink with white large radiating base with yellow center, Pink with dark pink radiating eye with pink center, Purple blue with white large radiating base with yellow center, and Pale pink with white eye with yellow center (Figure 2).…”

The Morphological and Anatomical Traits of the Leaf in Representative Vinca Species Observed on Indoor- and Outdoor-Grown Plants

“…There are quantitative and qualitative differences regarding the chemical composition of Vinca extracts, and previous studies showed that the morphological and anatomical traits of the leaves (shape and surface, cuticle, stomata, trichome, epidermis, etc.) are responsible for the chemical composition variability in the extracts [ 33 , 34 , 35 , 36 ]. These morphological and anatomical aspects of the Vinca leaf had been investigated in this study as well.…”

Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers

“…From each cultivar, 2-3 leaflets were taking, then the leaflets were cut in 2 x 2cm and then put in a plastic clip bag with silica gel with a volume ratio of 1: 5 to molecular analysis (Santoso et al 2003). Fresh leaf of the 5 th leaf from the shoot bud taken to anatomical profiles analysis (Samiyarsih et al 2019a). Detailed the sample site and the status of eight cultivars of sweet potato are presented in Table 1 and Figure 2.…”

“…Observations were made with a binocular microscope, Olympus CH-20, at 400x magnification. Measurement of anatomical profiles done using a calibrated ocular micrometer (Samiyarsih et al 2019a).…”

“…Sweet potatoes cultivar 'Cangkuang' have the best character on cuticle thickness, mesophyll thickness, stomata-trichomes density, and palisade ratio compared to other cultivars ( Figures 3.A and 4.A, Table 2). Samiyarsih et al (2019a) reported that the leaves have thick mesophyll indicated the availability of chloroplast in the palisade tissue. The high parenchyma sponge caused the high effectiveness of photosynthesis.…”