Variability of chromosomal DNA contents in maize ( Zea mays L.) inbred and hybrid lines

Lee, Jai‐Heon; Arumuganathan, K.; Kaeppler, Shawn M.; Park, Seong-Whan; Kim, Kee‐Young; Chung, Youngjin; Kim, Doh-Hoon; Fukui, Kiichi

doi:10.1007/s00425-002-0793-6

Cited by 40 publications

(24 citation statements)

References 34 publications

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“…Recent studies have documented differences in the content for several classes of repetitive DNA between maize inbreds at the chromosomal level (Kato et al 2004). Flow cytometry studies have also documented significant variation in the size of the maize genome between different inbred lines (Laurie and Bennett 1985;Lee et al 2002). Sequence-based methodologies have documented maize genome structural diversity at a higher resolution (for reviews, see Buckler et al 2006;Messing and Dooner 2006).…”

Section: Structural Diversity In Maizementioning

confidence: 99%

Allelic variation and heterosis in maize: How do two halves make more than a whole?

Springer¹,

Stupar²

2007

Genome Res.

339

252

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In this review, we discuss the recent research on allelic variation in maize and possible implications of this work toward our understanding of heterosis. Heterosis, or hybrid vigor, is the increased performance of a hybrid relative to the parents, and is a result of the variation that is present within a species. Intraspecific comparisons of sequence and expression levels in maize have documented a surprisingly high level of allelic variation, which includes variation for the content of genic fragments, variation in repetitive elements surrounding genes, and variation in gene expression levels. There is evidence that transposons and repetitive DNA play a major role in the generation of this allelic diversity. The combination of allelic variants provides a more comprehensive suite of alleles in the hybrid that may be involved in novel allelic interactions. A major unresolved question is how the combined allelic variation and interactions in a hybrid give rise to heterotic phenotypes. An understanding of allelic variation present in maize provides an opportunity to speculate on mechanisms that might lead to heterosis. Variation for the presence of genes, the presence of novel beneficial alleles, and modified levels of gene expression in hybrids may all contribute to the heterotic phenotypes.

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Section: Structural Diversity In Maizementioning

confidence: 99%

Allelic variation and heterosis in maize: How do two halves make more than a whole?

Springer¹,

Stupar²

2007

Genome Res.

339

252

View full text Add to dashboard Cite

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“…Seedlings were then treated with 1.0 µ M trifluralin (DowElanco, Midland, MI, USA), kept at room temperature for 4 h, washed by distilled water, and placed in ice water for 20-24 h. Then the root tips were excised and fixed with 2% paraformaldehyde in MgSO 4 buffer (50 mM KCl, 10 mM MgSO 4 7H 2 O, 5 mM HEPES, pH 8.0) for 20 min at room temperature. After washing three times with MgSO 4 buffer for 5 min each, meristems (1-1.5 mm) of 40 root types were dissected and transferred into a 1.5 mL Eppendolf tube containing 0.5 mL isolation buffer (50 mM KCl, 10 mM MgSO 4 7H 2 O, 5 mM HEPES, 3 mM dithiothreitol, 0.25% Triton-X 100, pH 8.0) (Lee et al, 2002).…”

Section: Methodsmentioning

confidence: 99%

Fluorescent labeling of plant chromosomes in suspension by FISH

Lee

et al. 2005

Genes Genet. Syst.

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By optimizing the concentration and time of treatment with hydroxyurea (HU), a DNA synthesis inhibitor, and trifluralin, a microtubule inhibitor, a highly effective (over 60%) cell cycle synchronization method for rye and barley meristem cells was developed. Chromosome suspensions containing highly purified and morphologically intact rye and barley chromosomes were prepared from the meristems of their root tips by homogenization. Digoxigenin-labeled 5S rDNA was used as a probe in FISH for the rye chromosomes in the suspension, and biotin-labeled 17S rDNA and centromeric DNA were used in FISH for the rye and barley chromosome suspensions, respectively. Bright signals were detected at the specific regions of interest on the chromosomes. The results indicate that the method developed in this study is useful for selection and sorting of chromosomes that are not distinguishable by other means, using specific fluorescent labeling by FISH of the chromosomes in suspension.

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“…The alkaloid Lee et al (1996Lee et al ( , 2002 *Number of chromosomes in a haploid set. **Number of chromosomes that could be discriminated unambiguously.…”

Section: Accumulation Of Mitotic Cells In Metaphasementioning

confidence: 99%

“…Since then, a whole range of cytogenetic stocks, including deletions, translocation and chromosome additions, has been found useful to discriminate speci¢c chromosomes and chromosome arms in a variety of species (Table 1 Gill , Li et al 2001, Kubala¤ kova¤ et al 2002, 2003a, 2003b. Simulated £ow karyotypes have been employed to predict a feasibility of discriminating individual chromosome types depending on di¡erences in relative DNA content and on the coe⁄cient of variation of the chromosome peaks (Lysa¤ k et al 1999, Lee et al 2002). The karyotypes were modelled using computer spreadsheet software (Conia et al 1989) or a dedicated computer program (Dolez› el 1991) based on relative chromosome length or relative chromosomal DNA content.…”

Section: Flow Karyotyping and Discrimination Of Single Chromosome Typesmentioning

confidence: 99%

Flow cytogenetics and plant genome mapping

et al. 2004

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The application of flow cytometry and sorting (flow cytogenetics) to plant chromosomes did not begin until the mid-1980s, having been delayed by difficulties in preparation of suspensions of intact chromosomes and discrimination of individual chromosome types. These problems have been overcome during the last ten years. So far, chromosome analysis and sorting has been reported in 17 species, including major legume and cereal crops. While chromosome classification by flow cytometry (flow karyotyping) may be used for quantitative detection of structural and numerical chromosome changes, chromosomes purified by flow sorting were found to be invaluable in a broad range of applications. These included physical mapping using PCR, high-resolution cytogenetic mapping using FISH and PRINS, production of recombinant DNA libraries, targeted isolation of markers, and protein analysis. A great potential is foreseen for the use of sorted chromosomes for construction of chromosome and chromosome-armspecific BAC libraries, targeted isolation of low-copy (genic) sequences, high-throughput physical mapping of ESTs and other DNA sequences by hybridization to DNA arrays, and global characterization of chromosomal proteins using approaches of proteomics. This paper provides a comprehensive review of the methodology and application of flow cytogenetics, and assesses its potential for plant genome analysis.

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Variability of chromosomal DNA contents in maize ( Zea mays L.) inbred and hybrid lines

Cited by 40 publications

References 34 publications

Allelic variation and heterosis in maize: How do two halves make more than a whole?

Allelic variation and heterosis in maize: How do two halves make more than a whole?

Fluorescent labeling of plant chromosomes in suspension by FISH

Flow cytogenetics and plant genome mapping

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