Variability of IgE reactivity profiles among European mite allergic patients

Abstract

Purified Der p 1 and Der p 2 are sufficient for the diagnosis of > or = 97% of D. pteronyssinus allergic patients in Europe, but other allergens may also play an important role for the diagnosis and treatment of HDM allergy.

Isoallergen Variations Contribute to the Overall Complexity of Effector Cell Degranulation: Effect Mediated through Differentiated IgE Affinity

“…Still, there is a considerable level of Ab cross-reactivity between the closely related group 2 HDM allergens Der p 2, Der f 2, and Eur m 2 (.80% amino acid sequence identity) (4,(8)(9)(10)(11). Conversely, Ab cross-reactivity between Der p 2 and the more distant related group 2 storage mite allergens Lep d 2, Tyr p 2, and Gly d 2 is low, consistent with a low (40%) sequence similarity between Der p 2 and these allergens (2,9).…”

Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

“…The frequency of IgE recognition, allergenic activity and clinical relevance of the major HDM allergens, Der p 1 and Der p 2, has been well investigated [5][6][7][8]. Both allergens are recognized by more than 90% of HDM-allergic patients and are considered as the major HDM allergens [5]. More than 20 other mite allergens have been identified but their importance for mite allergy has only partly been studied [9].…”

“…HDM allergens are among the most common triggers of asthma and allergic rhinitis [3,4]. The frequency of IgE recognition, allergenic activity and clinical relevance of the major HDM allergens, Der p 1 and Der p 2, has been well investigated [5][6][7][8]. Both allergens are recognized by more than 90% of HDM-allergic patients and are considered as the major HDM allergens [5].…”

Identification of Der p 23, a Peritrophin-Like Protein, As a New Major Dermatophagoides Pteronyssinus Allergen Associated with the Peritrophic Matrix of Mite Fecal Pellets

“…In fact, it is known that a mix of Der p 1 and Der p 2 allows diagnosing the majority of HDM allergic patients (30). However, a molecular or epitope mixture for specific immunotherapy certainly needs to include Der p 23 because of its high allergenic activity, its high frequency of recognition and due to the high levels of IgE-antibodies directed to this allergen.…”

“…The diagnosis of allergy to D. pteronyssinus was based on case history, typical indoor symptoms (rhinitis, conjunctivitis, and/or asthma), and the presence of D. pteronyssinusspecific IgE Abs > 0.7 kU A /l in serum as determined with the ImmunoCAP System (Thermofisher/Phadia, Uppsala, Sweden). Sera from nonallergic individuals were used for control purposes (30). The IgE binding frequency of Der p 23 was determined in a nondenaturing dot-blot assay.…”