Variability of Phenolic Compound Accumulation and Antioxidant Activity in Wild Plants of Some Rumex Species (Polygonaceae)

Plantago lanceolata L. (plantain) is an interesting multipurpose perennial species whose aerial parts are used in herbal medicine due to its precious phytochemicals and are palatable to animals. Moreover, peculiar traits such as drought tolerance, an extended growth season and a deep root system, make plantain a promising pioneer plant for quarry reclamation based on the use of native species. This study evaluated the effects of different environmental conditions and seasons on the accumulation of the bioactive compounds of its aerial organs. An autochthonous plantain population was grown in three locations in Sardinia (Italy). Leaves, peduncles and inflorescences were collected between October 2020 and July 2021. Phenolic contents and antioxidant capacity were determined. The analysis of the individual phenolic compounds was performed using liquid chromatography. In leaves, the content of total phenolics, antioxidant capacity and total flavonoids were significantly influenced by location and season. Total phenolic content ranged from 65 to 240 g gallic acid equivalent kg−1, whereas total flavonoids were from 16 to about 89 g catechin equivalent kg−1. Neochlorogenic, chlorogenic, cryptochlorogenic acids, verbascoside, diosmin and luteolin were identified in the methanolic extracts of leaves, peduncles and inflorescences. Verbascoside was the main antioxidant isolated from plantain extracts. Results evidenced an increasing accumulation pattern of phenolics from vegetative stage to flowering, followed by a decrement towards the seed ripening as well as site-specific differences with amounts of phenolics even 25% higher for same plantain accession.

Section: Resultssupporting

confidence: 94%

Antioxidant Contents in a Mediterranean Population of Plantago lanceolata L. Exploited for Quarry Reclamation Interventions

Sanna

Piluzza

Campesi

et al. 2022

“…Antioxidant activity depends on the content of different classes of phytocomponents in the samples. Typically, phenolic compounds have a significant effect on the total antioxidant activity of extracts [50,51].…”

Section: Correlation Between Phenolic Compounds Content and Antioxida...mentioning

confidence: 99%

“…The research team used the spectrophotometric method using the Arno reagent to determine the total hydroxycinnamic acid content [51,81]. Then, 1 mLof vegetable extract, 2 mL of 0.5 M HCl, 2 mL of Arno reagent, and 2 mL of 8.5% NaOH were added to 10 mL tubes.…”

Section: The Determination Of Total Content Of Hydroxycinnamic Acids ...mentioning

confidence: 99%

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The Content of Certain Groups of Phenolic Compounds and the Biological Activity of Extracts of Various Halophyte Parts of Spergularia marina (L.) Griseb. and Glaux maritima L. at Different Levels of Soil Salinization

Pungin

Lartseva

Loskutnikova

et al. 2022

Self Cite

Halophyte plants are known for their resistance to harsh environmental conditions associated with excess salts in their habitats. Their resistance to salinization is due, among other things, to their high ability to detoxify free radicals, owing to the relatively high content of antioxidants. On the coast of the Baltic Sea and in the lagoons, there are several rare halophyte species included in the Red Book of the Kaliningrad Region (Russia) and the Baltic region, such as Spergularia marina (L.) Griseb. and Glaux maritima L. The aim of the research was to study the accumulation of certain groups of phenolic compounds in different parts of S. marina and G. maritima plants under conditions of weak and strong soil salinity, as well as to analyze the antioxidant, antibacterial, and fungicidal activity of extracts of the studied plant species. The present study showed an increase in total phenolic content in the roots and shoots of S. marina, and the shoots of G. maritima, in response to increased soil salinity. At the same time, the total content of flavonoids in all the studied parts of the two plant species remained unchanged. However, the content of individual flavonoids (hesperetin, epicatechin, apigenin derivative, luteolin derivative) in S. marina increased, for G. maritima there was a tendency to reduce the content of flavonoids in roots and shoots with an increase in soil salinity. There was an increase in the total content of hydroxycinnamic acids in the roots of Glaux maritima, as well as an increase in the content of protocatechuic acid in the roots and shoots of Spergularia marina. A positive relationship was established between the antioxidant activity of S. marina root extracts and the total content of phenolic compounds, as well as G. maritima shoots extracts and the total content of phenolic compounds. Extracts of S. marina showed no antibacterial activity against Escherichia coli and Bacillus subtilis, and weak fungicidal activity of stem extracts and inflorescences grown on soils, with high levels of salinities, was detected against Candida albicans. The extracts of roots and shoots from G. maritima showed weak antimicrobial and fungicidal activity.

“…To determine the antioxidant activity, the extracts prepared as described in Section 4.2.1 were used. The antioxidant activity of the extracts was determined by the ability to capture radicals of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazolino-6-sulfonic acid (ABTS), as well as by the reducing power when interacting with the Fe(III)-2,4,6-tripyridyl-s-triazine complex (FRAP), according to [ 54 ].…”

Section: Methodsmentioning

confidence: 99%

Biotechnological Potential of Different Organs of Mistletoe (Viscum album L.) Collected from Various Host Tree Species in an Urban Area

Skrypnik

Feduraev

Golovin

et al. 2022

From an economic and ecological standpoint, it is crucial to investigate the biologically active compounds of mistletoe plants, which are currently discarded by pruning urban mistletoe-infested trees. In the present study, the content of phenolic compounds, triterpenic and organic acids, as well as the antioxidant activity of the extracts of various mistletoe organs (leaves, stems, and fruits) collected from the most infested tree species were investigated. The mistletoe samples collected from Betula pendula, Acer platanoides, Crataegus monogyna, and Sorbus aucuparia showed the highest content of phenolic acids and flavonoids as well as antioxidant activity, as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing/antioxidant power (FRAP) assays. The leaves and stems of mistletoe from Tilia cordata were characterized by a high content of triterpenic acids (oleanolic, ursolic, and betulinic). The leaves and fruits of mistletoe plants from Populus nigra and Salix alba contained a high concentration of organic acids, particularly succinic and citric acids. Compared to stem and leaf extracts, the antioxidant activity of the mistletoe fruit extracts was 1.5–3 times higher. The obtained results indicate that mistletoe is a valuable raw material and can be used as a source of phenolic compounds and triterpenic and organic acids, as well as for producing extracts with antioxidant properties.