Variability of Platelet-rich Plasma Preparations

Russell, Ryan P.; Apostolakos, John; Hirose, Tomoko; Cote, Mark P.; Mazzocca, Augustus D.

doi:10.1097/jsa.0000000000000007

Cited by 77 publications

(74 citation statements)

References 41 publications

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“…leukocytes. It has been demonstrated that the optimal platelet concentration corresponds to 3 to 4 198 times the blood concentration (<10 6 platelets/µL) 24,25 . However, more than 1.2 x 10 6 platelet/µL 199 lead to an inhibition of collagen synthesis 23,25 .…”

Section: Materials and Methods: 78mentioning

confidence: 99%

“…It has been demonstrated that the optimal platelet concentration corresponds to 3 to 4 198 times the blood concentration (<10 6 platelets/µL) 24,25 . However, more than 1.2 x 10 6 platelet/µL 199 lead to an inhibition of collagen synthesis 23,25 . White blood cells could adversely affect wound 200 healing through the release of pro-inflammatory factors responsible for extracellular matrix A c c e p t e d M a n u s c r i p t degradation 27 .…”

Section: Materials and Methods: 78mentioning

confidence: 99%

See 1 more Smart Citation

Using platelet-rich plasma to treat jumper's knees: Exploring the effect of a second closely-timed infiltration

Kaux¹,

Croisier

Forthomme

et al. 2016

Journal of Science and Medicine in Sport

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Section: Materials and Methods: 78mentioning

confidence: 99%

Section: Materials and Methods: 78mentioning

confidence: 99%

Using platelet-rich plasma to treat jumper's knees: Exploring the effect of a second closely-timed infiltration

Kaux¹,

Croisier

Forthomme

et al. 2016

Journal of Science and Medicine in Sport

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“…Differences in patients at the time of the blood draw result in plasma preparations that vary both within and between patients. This variability is compounded by the multitude of protocols and devices available for procuring PRP [598]. Criteria for PRP dosing and for tailoring preparations to each pathological situation have yet to be established.…”

Section: Formulations Enriched With Endogenous Growth Factorsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…Furthermore, different preparation methods aggravate the wide variability in the composition of different PRP products [13]. Kushida et al [14] reported great differences in growth factor concentrations in the activated PRP of seven commercial preparation systems.…”

Section: Introductionmentioning

confidence: 99%

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

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Variability of Platelet-rich Plasma Preparations

Cited by 77 publications

References 41 publications

Using platelet-rich plasma to treat jumper's knees: Exploring the effect of a second closely-timed infiltration

Using platelet-rich plasma to treat jumper's knees: Exploring the effect of a second closely-timed infiltration

Advancing biomaterials of human origin for tissue engineering

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

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