Variable estimates of cytokine levels produced by commercial ELISA kits: results using international cytokine standards

Ledur, Annick; Fitting, Catherine; David, B.; Hamberger, C.‐A.; Cavaillon, Jean‐Marc

doi:10.1016/0022-1759(95)00184-c

Cited by 117 publications

(61 citation statements)

References 26 publications

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“…Finally, antibody-based assays like ELISA and MIA render semiquantitative results. Although relative sample differences can be compared, absolute concentrations vary even between ELISA kits (24,25 ). One of the reasons for the latter is illustrated by the MIA-ELISA correlation studies we performed.…”

Section: Discussionmentioning

confidence: 99%

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A Multiplex Immunoassay for Human Adipokine Profiling

Bateman

Jager²,

Dijk³

et al. 2010

Clinical Chemistry

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BACKGROUND:Adipose tissue secretory proteins, called adipokines, play pivotal roles in the pathophysiology of obesity and its associated disorders such as metabolic syndrome, type 2 diabetes, and cardiovascular disease. Because methods for comprehensive adipokine profiling in patient plasma and other biological samples are currently limited, we developed a multiplex immunoassay for rapid and high-throughput measurement of 25 adipokines in only 50 L of sample.

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Section: Discussionmentioning

confidence: 99%

“…One of the reasons for the latter is illustrated by the MIA-ELISA correlation studies we performed. As described earlier, comparing MIA and/or ELISA assays using different antibody clones renders lower correlations (12,13,22,24,25 ). Meaningful MIA and/or ELISA comparisons for validation purposes thus require analogous antibody clones.…”

Section: Discussionmentioning

confidence: 99%

A Multiplex Immunoassay for Human Adipokine Profiling

Bateman

Jager²,

Dijk³

et al. 2010

Clinical Chemistry

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“…Using the sera obtained at the time point before MRA administration, serum concentrations of IL-6, sIL-6R, and MRA (anti-IL-6R antibody) were also determined by enzymelinked immunosorbent assay as previously described (13,15,16).…”

Section: Methodsmentioning

confidence: 99%

Therapeutic efficacy of humanized recombinant anti–interleukin‐6 receptor antibody in children with systemic‐onset juvenile idiopathic arthritis

Yokota

Miyamae

Imagawa

et al. 2005

Arthritis & Rheumatism

352

199

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Objective. To investigate the safety and efficacy of a recombinant human anti-interleukin-6 (anti-IL-6) receptor monoclonal antibody (MRA) that indirectly inhibits the effects of IL-6 in children with systemiconset juvenile idiopathic arthritis (JIA) refractory to high-dose, long-term corticosteroids.Methods. An individual escalating-dose trial was conducted in 11 children with active systemic-onset JIA who met the inclusion criteria. All were first administered an intravenous dose of 2 mg/kg MRA. Each child without active inflammation was given a second identical dose 2 weeks later and a third identical dose 2 weeks after the second dose. Children with disease flares according to laboratory marker values received a 4-mg/kg dose. Those without disease flares at this dose received a second 4-mg/kg dose 2 weeks later and a third 4-mg/kg dose 2 weeks after the second dose, while those with active inflammation received an additional 3 doses of 8 mg/kg MRA. Efficacy was evaluated every 2 weeks according to responses on the JIA core set of improvement criteria and the results of laboratory tests.Results. MRA abruptly reduced disease activity in 10 of the 11 children, as assessed by the occurrence of febrile episodes, active arthritis, scores on the Childhood Health Assessment Questionnaire, and levels of acute-phase reactants. However, levels of inflammatory reactants fluctuated until the proper MRA dose for each child was reached. Two weeks after the third fixed dose of MRA, 90.9% of all patients had a 30% improvement response, 90.9% had a 50% improvement response, and 63.6% had a 70% improvement response.Conclusion. MRA treatment of children with active systemic disease results in clinical improvement and in normalized levels of acute-phase reactants. MRA was safe and well tolerated and provided greater clinical benefit than conventional corticosteroids, considering the ill effects of IL-6 and adverse events.

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“…This would require retest on diluted sample for high outliers and alternate testing for low outliers. Furthermore, it has been reported that cytokine values may vary among kits from different manufacturers and quantitative techniques and the kits from the same supplier should be used to compare the absolute values for ELISA and Luminex measurements [12] [20]. Therefore, it is important to realize that absolute cytokine value results obtained by different methods might not be the same, but the trend of the values among experiment groups are comparable.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of Enzyme-Linked Immunosorbent Assay versus Multiplex Methodology Using an <i>in Vitro</i> Model of Pulmonary Hypertension and Inflammation

Zhu¹,

Alapati²,

Costa³

et al. 2014

JBiSE

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Variable estimates of cytokine levels produced by commercial ELISA kits: results using international cytokine standards

Cited by 117 publications

References 26 publications

A Multiplex Immunoassay for Human Adipokine Profiling

A Multiplex Immunoassay for Human Adipokine Profiling

Therapeutic efficacy of humanized recombinant anti–interleukin‐6 receptor antibody in children with systemic‐onset juvenile idiopathic arthritis

A Comparison of Enzyme-Linked Immunosorbent Assay versus Multiplex Methodology Using an <i>in Vitro</i> Model of Pulmonary Hypertension and Inflammation

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Variable estimates of cytokine levels produced by commercial ELISA kits: results using international cytokine standards

Cited by 117 publications

References 26 publications

A Multiplex Immunoassay for Human Adipokine Profiling

A Multiplex Immunoassay for Human Adipokine Profiling

Therapeutic efficacy of humanized recombinant anti–interleukin‐6 receptor antibody in children with systemic‐onset juvenile idiopathic arthritis

A Comparison of Enzyme-Linked Immunosorbent Assay versus Multiplex Methodology Using an &lt;i&gt;in Vitro&lt;/i&gt; Model of Pulmonary Hypertension and Inflammation

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A Comparison of Enzyme-Linked Immunosorbent Assay versus Multiplex Methodology Using an <i>in Vitro</i> Model of Pulmonary Hypertension and Inflammation