Variable fidelity of tissue‐marking dyes in surgical pathology

Williams, Andrew S.; Haché, Kelly Dakin

doi:10.1111/his.12328

Cited by 16 publications

(13 citation statements)

References 16 publications

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“…india ink is the traditional marking ink in the field of surgical pathology (1,2). Other marking techniques include painting with alcian blue, plain gelatine, gelatine with added colours, TMD, erythrocytes and surface coating with starch (3,6,7).…”

Section: Discussionmentioning

confidence: 99%

“…Histopathological assessment of radical or wide excision specimens requires proper orientation of the specimen along with identification of all surgical margins (1)(2)(3)(4)(5)(6)(7)(8). Several methods have been utilised for margin assessment like the marking of surgical margins with sutures and clips or applying india ink (1,2).…”

Section: Introductionmentioning

confidence: 99%

“…Though tissue marking dyes (TMD) are available in the market, they are expensive and not readily available (2,3,7,8). acrylic colours are easily available in numerous colours, and are affordable, non-toxic, non-flammable and stable (1,3,6,7,10) (Figure 1a-E).…”

Section: Introductionmentioning

confidence: 99%

“…acrylic colours are easily available in numerous colours, and are affordable, non-toxic, non-flammable and stable (1,3,6,7,10) (Figure 1a-E). However, not all acrylic colours withstand tissue processing and fulfill the criteria of being used as surgical ink; plus though the inking has to be done before grossing, whether application of ink after overnight fixation is better or inking followed by fixation and grossing is preferable is to be sought.…”

Section: Introductionmentioning

confidence: 99%

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Inking in surgical pathology: does the method matter? a procedural analysis of a spectrum of colours

Pursnani¹,

Arora²,

Katyayani³

et al. 2016

TJPATH

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Objective: Proper identification of surgical margins along with margin status holds utmost importance in histopathology. inking margins is one way. india ink has long been used but it can ink only one margin. On other hand acrylic colours, available in variety of colours can be used for inking multiple margins along with many more advantages. The present study was undertaken to analyse acrylic colours and indian ink for inking surgical margins via three different methods for optimum results. Material and Method:Thirteen acrylic colours along with india ink were evaluated via three different methods on radical specimens of breast and colon after preliminary requisites of grossing were completed. in Method 1, coloured inks were applied to an overnight formalin fixed specimen and representative sections were taken. in Method 2, the specimen was inked and kept for overnight fixation. The specimen was sampled following day. in Method 3, the specimen was inked and kept for overnight fixation. The following day, it was re-coloured with the same colours as of the previous day and subsequently sections were taken. Coloured inks were assessed on different parameters for their performance as surgical ink and given scores.Results: acrylic shades #04, #22, #06, #01, #02 and india ink had very good to excellent score on microscopy in all three methods. Shades #64, #18, #09, #23 had poor microscopic visibility in all three methods. Shade #09 showed loss of colour and the shade #23 showed penetration into deeper tissues on microscopy. results were best with Method 3 followed by Method 1 and 2, respectively. Conclusion: acrylic colours have more advantages compared to india ink. Method 3 is recommended for inking. Few acrylic shades meet the criteria of surgical inks in all three methods.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inking in surgical pathology: does the method matter? a procedural analysis of a spectrum of colours

Pursnani¹,

Arora²,

Katyayani³

et al. 2016

TJPATH

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show abstract

“…Acrylic colors have many advantages as compared to others in terms of cost effectiveness, availability, reproducibility of results and ease of application. [4][5][6] India ink imparts a single colour whereas acrylic colors are available in different shades of colors where it can be easily applied for identifying various surgical margins of radical specimen in cases of malignancy. [2] For routine histopathology specimens, for example in case of appendix and fallopian tube, multiple sections are taken and embedded in paraffin blocks then slides are prepared for each.…”

Section: Introductionmentioning

confidence: 99%

Surgical Inking and Double Embedding Technique for Routine Histopathology to Save Time and Effort

Nerune

Mestri

Konduru

et al. 2018

APALM

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Background: Routine histopathological specimens like appendix and fallopian tube require individual processing, block making and slide preparation. This process is not only time consuming and requires more effort but also takes up more space during archiving. The objective of the present study was to use commercially available different acrylic colors to ink specimen of appendix and fallopian tube of different patients, process them in a single cassette and preparation of single paraffin block and slide.Methods: 100 cases of appendix and fallopian tubes were taken to evaluate ink mapping technique using Fevicryl® Acrylic colours. Blocks and slides were prepared, labelling on the slides was done using the chromacodes method for identification of the specimen. These slides were then reported by the pathologist.Result: There was a 21% reduction in the amount of paraffin required for preparation of a double embedded block in comparison to two paraffin blocks by conventional method. It was also noted that there was 18% reduction in volume as compared to two conventional paraffin blocks. Conclusion:This method of using surgical inking technique not only brings down the effort and time required, but also storage space and the costs involved along with faster identification and reporting compared to the conventional method.

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Identification of Optimal Tissue-Marking Dye Color for Pathological Evaluation in Fluorescence Imaging Using IRDye800CW

Kondo,

Nishio,

Park

et al. 2023

Mol Imaging Biol

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Variable fidelity of tissue‐marking dyes in surgical pathology

Cited by 16 publications

References 16 publications

Inking in surgical pathology: does the method matter? a procedural analysis of a spectrum of colours

Inking in surgical pathology: does the method matter? a procedural analysis of a spectrum of colours

Surgical Inking and Double Embedding Technique for Routine Histopathology to Save Time and Effort

Identification of Optimal Tissue-Marking Dye Color for Pathological Evaluation in Fluorescence Imaging Using IRDye800CW

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