Variant rs8400 enhances ALKBH5 expression through disrupting miR-186 binding and promotes neuroblastoma progression

Guan, Qian; Lin, Huiran; Hua, Wenfeng; Lin, Lei; Liu, Jiabin; Deng, Linqing; Zhang, Jiao; Cheng, Jiwen; Yang, Zhonghua; Li, Yong; Bian, Jun; Zhou, Haixia; Li, Suhong; Li, Li; Miao, Lei; Xia, Huimin; He, Jing; Zhang, Zhuo

doi:10.21147/j.issn.1000-9604.2023.02.05

Cited by 25 publications

(17 citation statements)

References 60 publications

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“…Pathological sequential slides with a thickness of 4 μm were sectioned from formalin-fixed, paraffin-embedded (FFPE) tumor tissues for IHC staining following the previous protocol [ [17] , [18] , [19] ]. Briefly, all tumor sections were sequentially followed with deparaffinization, rehydration, and then pre-incubation with hydrogen peroxide.…”

Section: Methodsmentioning

confidence: 99%

CD20highCD138low tumor-infiltrating lymphocytes predominantly related to cytokine‒cytokine receptor interactions are associated with favorable outcomes in neuroblastoma patients

Qin,

Xu,

et al. 2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

CD20highCD138low tumor-infiltrating lymphocytes predominantly related to cytokine‒cytokine receptor interactions are associated with favorable outcomes in neuroblastoma patients

Qin,

Xu,

et al. 2024

Heliyon

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“…The purity and concentration of the extracted DNA were further measured by using UV spectrophotometer (Nano Drop Technologies, Inc., Wilmington, DE). We used Taqman real‐time PCR method (Applied Biosystems, CA, USA) to determine the SNP genotype in samples, which has been mentioned previously 30–32 . Regarding quality control, 10% of the genotyping samples were randomly retested, and a genotype consensus rate of 100% was attained.…”

Section: Methodsmentioning

confidence: 99%

miR‐100 rs1834306 a > G polymorphism decreases neuroblastoma risk in Chinese children

Han,

Chang,

Lin

et al. 2023

Cancer Reports

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BackgroundNeuroblastoma is a common malignant tumor stemming from the sympathetic nervous system in children, which is often life‐threatening. The genetics of neuroblastoma remains unclear. Studies have shown that miRNAs participate in the regulation of a broad spectrum of biological pathways. The abnormity in the miRNA is associated with the risk of various cancers, including neuroblastoma. However, research on the relationship of miRNA polymorphisms with neuroblastoma susceptibility is still in the initial stage.MethodsIn this research, a retrospective case–control study was conducted to explore whether miR‐100 rs1834306 A > G polymorphism is associated with neuroblastoma susceptibility. We enrolled 402 cases and 473 controls for the study. The logistic regression analysis was adopted to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for the association between miR‐100 rs1834306 A > G and neuroblastoma risk.ResultsOur results elucidated that the miR‐100 rs1834306 A > G polymorphism was associated with the decreased risk of neuroblastoma (AG versus AA: adjusted OR = 0.72, 95% CI = 0.53–0.98, and P = 0.038). The subsequent stratified analysis further found that rs1834306 AG/GG genotype reduced the risk of neuroblastoma in the subgroup with tumors of the mediastinum origin (adjusted OR = 0.63, 95% CI = 0.41–0.95, and P = 0.029).ConclusionsIn summary, miR‐100 rs1834306 A > G polymorphism was shown to associate with decreased neuroblastoma risk in Chinese children, especially for neuroblastoma of mediastinum origin. This conclusion needs to be verified in additional large‐size case–control studies.

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“…Taking advantage of their abundant presence in genomes, potential functional impact in coding regions and linkage disequilibrium‐derived haplotypes, SNPs offer a distinct contribution to seek an instructive biotarget in the occurrence and progression of disease. For example, variant rs8400 has been demonstrated to affect the activity of miR‐186‐3p, thereby dysregulating ALKBH5 and promoting the progression of neuroblastoma through the ALKBH5‐SPP1 axis 19 . Therefore, it is advisable to further investigate the molecular mechanisms underlying disease etiology by focusing on informative SNPs.…”

Section: Gene Polymorphism Relevance Referencementioning

confidence: 99%

“…For example, variant rs8400 has been demonstrated to affect the activity of miR-186-3p, thereby dysregulating ALKBH5 and promoting the progression of neuroblastoma through the ALKBH5-SPP1 axis. 19 Therefore, it is advisable to further investigate the molecular mechanisms underlying disease etiology by focusing on informative SNPs. Additionally, the use of polygenic risk scores (PRS) as an alternative approach is available.…”

Section: Genementioning

confidence: 99%

Unraveling genetic contributors to bleeding events induced by rivaroxaban treatment

Chen

Deng

Miao

et al. 2023

Clinical and Translational Dis

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Variant rs8400 enhances ALKBH5 expression through disrupting miR-186 binding and promotes neuroblastoma progression

Cited by 25 publications

References 60 publications

CD20highCD138low tumor-infiltrating lymphocytes predominantly related to cytokine‒cytokine receptor interactions are associated with favorable outcomes in neuroblastoma patients

CD20highCD138low tumor-infiltrating lymphocytes predominantly related to cytokine‒cytokine receptor interactions are associated with favorable outcomes in neuroblastoma patients

miR‐100 rs1834306 a > G polymorphism decreases neuroblastoma risk in Chinese children

Unraveling genetic contributors to bleeding events induced by rivaroxaban treatment

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