Variants in ZNRD1 Gene Predict HIV-1/AIDS Disease Progression in a Han Chinese Population in Taiwan

Abstract

Patients demonstrate notable variations in disease progression following human immunodeficiency virus (HIV) infection. We aimed to identify ZNRD1 and RNF39 genetic variants linked to AIDS progression. We conducted a genetic association study in HIV-1-infected Han Chinese patients residing in Taiwan. The clinical characteristics of 143 HIV-1-infected patients were measured, and patients were split into 2 groups: AIDS progression and AIDS non-progression. Genotyping of ZNRD1 and RNF39 was performed in all participants. We found that patients in the AIDS progression group had higher HIV-1 viral loads and lower CD4 cell counts than did patients in the AIDS non-progression group. The frequency of the AA genotype of ZNRD1 (rs16896970) was lower in the AIDS progression group than in the AIDS non-progression group. Patients with AA genotypes had lower levels of HIV-1 viral loads and higher levels of CD4 cell counts than did patients with AG+GG genotypes. AIDS progression in patients with the AA group is significantly different from that in patients with the AG and GG groups by using Kaplan-Meier survival analysis. The hazard ratio for progression was lower in the AA group than in the AG and GG groups. We identified a SNP that contributes to AIDS progression in HIV-1-infected patients in this population. This SNP had a significant protective influence on AIDS progression, and polymorphisms of the ZNRD1 gene may play a role in the pathogenesis of HIV-1 infection.

Sorting nexin 24 genetic variation associates with coronary artery aneurysm severity in Kawasaki disease patients

“…The genotypes of the 9 genetic polymorphisms were identified by a custom-designed genotyping method [20]. The genotype frequencies of these polymorphisms are shown in Table 1.…”

Allelic variants of human genes affecting HIV intracellular life cycle and regulating immune response to HIV infection

“…Эффективность транскрипции провируса значительно варьирует у различных индивидуумов и определяет 64-83% от общей вариабельности вирусной нагрузки [59]. Ген ZNRD1 кодирует субъединицу РНК-полимеразы I. Механизм опосредуемого им ограничения ВИЧ-инфекции может быть связан с влиянием на процессинг транскриптов ВИЧ-1 с участием регуляторного белка Rev.…”

“…Экспрессия гена ZNRD1 ассоциирована с полиморфизмами rs3869068 и rs9261174, которые расположены в регуляторном 5′-регионе. Полиморфизмы rs1048412, rs16896970, rs9261174 гена ZNRD1 ассоциированы с замедлением развития СПИДа [48,59], причем полиморфизм rs1048412 имеет достоверную ассоциацию с LT-NP-фенотипом [60].…”

“…Ассоциации полиморфизмов гена RNF39 с устойчивостью или восприимчивостью к ВИЧ-инфекции недостаточно изучены, хотя выявлено большое их количество. Два полиморфизма rs2301753 и rs2074479 локализованы в кодирующем районе и приводят к заменам аминокислот [59].…”

Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture

“…We have also showed that ZNRD1 genetic variants contribute to HIV-1 clinical course in the Han Chinese population in Taiwan and that ZNRD1 RNA interference-mediated silencing inhibited HIV-1 replication in Jurkat cells [13]. RNF39 encodes a protein containing a specialized type of SPRY domain in its C-terminal that determines viral specificity and restriction potency [14].…”