Variation in Calvarial Bone Healing Capacity

Skogh, Ann-Charlott Docherty; Kihlström, Lars; Neovius, Erik; Persson, Cecilia; Beckman, Mats O.; Engstrand, Thomas

doi:10.1097/scs.0b013e31827ff2b6

Cited by 26 publications

(17 citation statements)

References 6 publications

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“…Recently, a novel polymer hydrogel of sugarcane molasses appeared to be a good candidate to treat calvarial bone defects in rats, in association with Bone Morphogenetic Proteins (BMPs) [43]. In patients, hyaluronan-based hydrogels associated with BMP-2 greatly enhanced the healing of critical-size cranial defects [44] or alveolar cleft defects [45], and alginate-agarose hydrogels combined with autologous chondrocytes significantly improved clinical outcome in patients suffering from chondral or osteochondral defects over a 2-year follow-up [46]. …”

Section: Discussionmentioning

confidence: 99%

Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats

Frasca¹,

Norol

Visage

et al. 2017

J Mater Sci: Mater Med

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Research in bone tissue engineering is focused on the development of alternatives to autologous bone grafts for bone reconstruction. Although multiple stem cell-based products and biomaterials are currently being investigated, comparative studies are rarely achieved to evaluate the most appropriate approach in this context. Here, we aimed to compare different clinically relevant bone tissue engineering methods and evaluated the kinetic repair and the bone healing efficiency supported by mesenchymal stem cells and two different biomaterials, a new hydrogel scaffold and a commercial hydroxyapatite/tricalcium phosphate ceramic, alone or in combination.Syngeneic mesenchymal stem cells (5 × 105) and macroporous biphasic calcium phosphate ceramic granules (Calciresorb C35®, Ceraver) or porous pullulan/dextran-based hydrogel scaffold were implanted alone or combined in a drilled-hole bone defect in rats. Using quantitative microtomography measurements and qualitative histological examinations, their osteogenic properties were evaluated 7, 30, and 90 days after implantation. Three months after surgery, only minimal repair was evidenced in control rats while newly mineralized bone was massively observed in animals treated with either hydrogels (bone volume/tissue volume = 20%) or ceramics (bone volume/tissue volume = 26%). Repair mechanism and resorption kinetics were strikingly different: rapidly-resorbed hydrogels induced a dense bone mineralization from the edges of the defect while ceramics triggered newly woven bone formation in close contact with the ceramic surface that remained unresorbed. Delivery of mesenchymal stem cells in combination with these biomaterials enhanced both bone healing (>20%) and neovascularization after 1 month, mainly in hydrogel.Osteogenic and angiogenic properties combined with rapid resorption make hydrogels a promising alternative to ceramics for bone repair by cell therapy.

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Section: Discussionmentioning

confidence: 99%

Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats

Frasca¹,

Norol

Visage

et al. 2017

J Mater Sci: Mater Med

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“…Hemostatic gelatin sponges have been demonstrated as a suitable in vitro model for producing 3-dimensional (3D) human and bovine chondrocyte cultures 21 22 23 . While some studies have demonstrated the usefulness of hemostatic gelatin sponges as a carrier or an implant for repairing gingival depressions and bone defects 24 25 26 27 , these studies only demonstrated the suitability of gelatin sponge as a carrier or an implant for bone regeneration. For example, Arias-Gallo et al .…”

mentioning

confidence: 99%

“…used gelatin sponges as a carrier of fibroblast growth factors 26 and Skogh et al . applied gelatin sponges as an implant for bone regeneration 27 . These studies did not demonstrate their usefulness in regards to cell differentiation.…”

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confidence: 99%

Osteogenic differentiation of preosteoblasts on a hemostatic gelatin sponge

Kuo

Lai

Toh

et al. 2016

Sci Rep

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Bone tissue engineering provides many advantages for repairing skeletal defects. Although many different kinds of biomaterials have been used for bone tissue engineering, safety issues must be considered when using them in a clinical setting. In this study, we examined the effects of using a common clinical item, a hemostatic gelatin sponge, as a scaffold for bone tissue engineering. The use of such a clinically acceptable item may hasten the translational lag from laboratory to clinical studies. We performed both degradation and biocompatibility studies on the hemostatic gelatin sponge, and cultured preosteoblasts within the sponge scaffold to demonstrate its osteogenic differentiation potential. In degradation assays, the gelatin sponge demonstrated good stability after being immersed in PBS for 8 weeks (losing only about 10% of its net weight and about 54% decrease of mechanical strength), but pepsin and collagenases readily biodegraded it. The gelatin sponge demonstrated good biocompatibility to preosteoblasts as demonstrated by MTT assay, confocal microscopy, and scanning electron microscopy. Furthermore, osteogenic differentiation and the migration of preosteoblasts, elevated alkaline phosphatase activity, and in vitro mineralization were observed within the scaffold structure. Each of these results indicates that the hemostatic gelatin sponge is a suitable scaffold for bone tissue engineering.

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“…Indeed, most of these studies underlined the benefits that the HA derivatives, either as hydrogel or solid mesh, exerted in human diseases such as venous ulcers [107],[108], decubitus ulcers [109], oral mucosal lesions [110], surgical ablation of nevi/cutaneous tumors and cicatritial outcomes [111], talar dome and knee osteochondral lesions [112],[113], and cranial bone regeneration [114]. So far, no clinical trial started to verify whether such biomaterial can be useful also to repair the infarcted myocardium.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan and cardiac regeneration

et al. 2014

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Hyaluronan (HA) is abundantly expressed in several human tissues and a variety of roles for HA has been highlighted. Particularly relevant for tissue repair, HA is actively produced during tissue injury, as widely evidenced in wound healing investigations. In the heart HA is involved in physiological functions, such as cardiac development during embryogenesis, and in pathological conditions including atherosclerosis and myocardial infarction. Moreover, owing to its relevant biological properties, HA has been widely used as a biomaterial for heart regeneration after a myocardial infarction. Indeed, HA and its derivatives are biodegradable and biocompatible, promote faster healing of injured tissues, and support cells in relevant processes including survival, proliferation, and differentiation. Injectable HA-based therapies for cardiovascular disease are gaining growing attention because of the benefits obtained in preclinical models of myocardial infarction. HA-based hydrogels, especially as a vehicle for stem cells, have been demonstrated to improve the process of cardiac repair by stimulating angiogenesis, reducing inflammation, and supporting local and grafted cells in their reparative functions. Solid-state HA-based scaffolds have been also investigated to produce constructs hosting mesenchymal stem cells or endothelial progenitor cells to be transplanted onto the infarcted surface of the heart. Finally, applying an ex-vivo mechanical stretching, stem cells grown in HA-based 3D scaffolds can further increase extracellular matrix production and proneness to differentiate into muscle phenotypes, thus suggesting a potential strategy to create a suitable engineered myocardial tissue for cardiac regeneration.Electronic supplementary materialThe online version of this article (doi:10.1186/s12929-014-0100-4) contains supplementary material, which is available to authorized users.

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Variation in Calvarial Bone Healing Capacity

Cited by 26 publications

References 6 publications

Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats

Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats

Osteogenic differentiation of preosteoblasts on a hemostatic gelatin sponge

Hyaluronan and cardiac regeneration

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