Variation in Endogenous Cytokinin Content during Adventitious Root Formation in Pea Cuttings

Bollmark, Marie; Kubát, B.; Eliasson, Lennart

doi:10.1016/s0176-1617(88)80102-0

Cited by 162 publications

(92 citation statements)

References 22 publications

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“…Ethylene biosynthesis is predicted to increase after shoot excision (Bollmark et al, 1988;O'Donnell et al, 1996;Van Loon et al, 2006). We observed an increase in Figure 1.…”

Section: The Poplar Transcriptome Is Remodeled In the Base Of Excisedmentioning

confidence: 56%

The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development inPopulus

et al. 2009

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Adventitious root formation at the base of plant cuttings is an innate de novo organogenesis process that allows massive vegetative propagation of many economically and ecologically important species. The early molecular events following shoot excision are not well understood. Using whole-genome microarrays, we detected significant transcriptome remodeling during 48 h following shoot removal in Populus tremula 3 Populus alba softwood cuttings in the absence of exogenous auxin, with 27% and 36% of the gene models showing differential abundance between 0 and 6 h and between 6 and 24 h, respectively. During these two time intervals, gene networks involved in protein turnover, protein phosphorylation, molecular transport, and translation were among the most significantly regulated. Transgenic lines expressing a constitutively active form of the Populus type-B cytokinin response regulator PtRR13 (DDDKPtRR13) have a delayed rooting phenotype and cause misregulation of CONTINUOUS VASCULAR RING1, a negative regulator of vascularization; PLEIOTROPIC DRUG RESISTANCE TRANS-PORTER9, an auxin efflux transporter; and two APETALA2/ETHYLENE RESPONSE FACTOR genes with sequence similarity to TINY. Inappropriate cytokinin action via DDDKPtRR13 expression appeared to disrupt adventitious root development 24 h after shoot excision, when root founder cells are hypothesized to be sensitive to the negative effects of cytokinin. Our results are consistent with PtRR13 acting downstream of cytokinin to repress adventitious root formation in intact plants, and that reduced cytokinin signaling after shoot excision enables coordinated expression of ethylene, auxin, and vascularization pathways leading to adventitious root development.

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“…Ethylene biosynthesis is predicted to increase after shoot excision (Bollmark et al, 1988;O'Donnell et al, 1996;Van Loon et al, 2006). We observed an increase in Figure 1.…”

Section: The Poplar Transcriptome Is Remodeled In the Base Of Excisedmentioning

confidence: 56%

The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development inPopulus

et al. 2009

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“…Adventitious roots forming from Arabidopsis hypocotyls initiate from a cell layer proximate to the central cylinder and in this regard show similarity to the lateral roots that emerge from pericycle founder cells (Casimiro et al 2001). In many important crop species (including rice, maize and wheat), adventitious roots are formed via vascular cambial cell divisions whereby phloem initials start to divide to produce a primordium (Naija et al 2009) callus in which tracheids differentiate, elongate and eventually form the center of a complete root primordium (Bollmark et al 1988;Lorbiecke and Sauter 1999;Rasmussen and Hunt 2010).…”

Section: Strigolactones Inhibit Adventitious Root Formationmentioning

confidence: 97%

Strigolactones fine-tune the root system

Rasmussen

Depuydt

Goormachtig

et al. 2013

Planta

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Strigolactones were originally discovered to be involved in parasitic weed germination, in mycorrhizal association and in the control of shoot architecture. Despite their clear role in rhizosphere signaling, comparatively less attention has been given to the belowground function of strigolactones on plant development. However, research has revealed that strigolactones play a key role in the regulation of the root system including adventitious roots, primary root length, lateral roots, root hairs and nodulation. Here, we review the recent progress regarding strigolactone regulation of the root system and the antagonism and interplay with other hormones

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“…Despite a long history of clonal propagation, we are only recently beginning to understand adventitious root formation at the genetic, molecular and biochemical levels. [1][2][3] Several hormones have been shown to control adventitious root formation and cytokinin, [7][8][9][10][11] however their mechanisms of action have yet to be fully elucidated and it can be expected that other signaling pathways play important roles. One signaling pathway that we showed recently to be a potent regulator of adventitious rooting is that of strigolactone.…”

mentioning

confidence: 99%

Inhibition of strigolactones promotes adventitious root formation

Rasmussen

Beveridge

Geelen

2012

Plant Signaling & Behavior

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Roots that form from non-root tissues (adventitious roots) are crucial for cutting propagation in the forestry and horticulture industries. Strigolactone has been demonstrated to be an important regulator of these roots in both Arabidopsis and pea using strigolactone deficient mutants and exogenous hormone applications. Strigolactones are produced from a carotenoid precursor which can be blocked using the widely available but broad terpenoid biosynthesis blocker, fluridone. We demonstrate here that fluridone can be used to promote adventitious rooting in the model species Pisum sativum (pea). In addition, in the garden species Plumbago auriculata and Jasminium polyanthum fluridone was equally as successful at promoting roots as a commercial rooting compound containing NAA and IBA. Our findings demonstrate that inhibition of strigolactone signaling has the potential to be used to improve adventitious rooting in commercially relevant species.Adventitious root formation is the process of root initiation from a non-root tissue and the ability to induce these roots is central to plant industries worldwide. However many plant species do not readily produce adventitious roots and considerably constraining forestry and horticulture industries and limiting the use of this approach in plant conservation. Despite a long history of clonal propagation, we are only recently beginning to understand adventitious root formation at the genetic, molecular and biochemical levels. [1][2][3]

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Variation in Endogenous Cytokinin Content during Adventitious Root Formation in Pea Cuttings

Cited by 162 publications

References 22 publications

The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development inPopulus

The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development inPopulus

Strigolactones fine-tune the root system

Inhibition of strigolactones promotes adventitious root formation

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