Variation in hydrogen peroxide sensitivity between different strains of Neisseria gonorrhoeae is dependent on factors in addition to catalase activity

Alcorn, Timothy M.; Zheng, Heyi; Gunther, Michael R.; Hassett, Daniel J.; Cohen, Myron S.

doi:10.1128/iai.62.5.2138-2140.1994

Cited by 14 publications

(4 citation statements)

References 18 publications

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“…In separate studies on V. cholera, exposure of 10 6 cells/ml to 1 mM H 2 O 2 resulted in greater than 99% reduction in CFU, whereas at a 10-fold higher cell density V. cholera removed the H 2 O 2 from the assay medium and suffered less than 10% reduction in CFU (Schott et al, unpublished). Similar ''threshold cell density'' effects have been reported by others (Alcorn et al, 1994). In this study, P. marinus was tested at 2.5 Â 10 6 cells/ml.…”

Section: Effects Of H 2 O 2 On Viability Of P Marinus and V Splendidussupporting

confidence: 83%

The protistan parasite Perkinsus marinus is resistant to selected reactive oxygen species

Schott¹,

Pecher²,

Okafor³

et al. 2003

Experimental Parasitology

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The parasite Perkinsus marinus has devastated natural and farmed oyster populations along the Atlantic and Gulf coasts of North America. When viable P. marinus trophozoites are engulfed by oyster hemocytes, the typical accumulation of reactive oxygen species (ROS) normally associated with phagocyte activity is not observed. One hypothesis to explain this is that the parasite rapidly removes ROS. A manifestation of efficient ROS removal should be a high level of resistance to exogenous ROS. We investigated the in vitro susceptibility of P. marinus to ROS as compared to the estuarine bacterium Vibrio splendidus. We find that P. marinus is markedly less susceptible than V. splendidus to superoxide and hydrogen peroxide (H(2)O(2)), but equally sensitive to hypochlorite. Viable P. marinus trophozoites degrade H(2)O(2) in vitro, but lack detectable catalase activity. However, extracts contain an ascorbate dependent peroxidase activity that may contribute to H(2)O(2) removal in vitro and in vivo.

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Section: Effects Of H 2 O 2 On Viability Of P Marinus and V Splendidussupporting

confidence: 83%

The protistan parasite Perkinsus marinus is resistant to selected reactive oxygen species

Schott¹,

Pecher²,

Okafor³

et al. 2003

Experimental Parasitology

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“…In addition, the Mc clinical isolates were subjected to oxidative stress by exposure to H 2 O 2. The Mc isolates exhibited differences in H 2 O 2 sensitivity, as observed in the close relative Neisseria gonorrhoeae (Alcorn et al , 1994), and no clear correlation could be detected between high or low mutation rates and hyper‐resistance or hyper‐sensitivity to H 2 O 2 (Table 4). In this context, it should be pointed out that Mc, in addition to catalase and a number of other scavenger enzymes (Seib et al , 2004), hosts no fewer than three DsbA homologues (Sinha et al , 2004), rendering this species particularly fit for handling oxidative stress.…”

Section: Resultsmentioning

confidence: 94%

DNA repair profiles of disease-associated isolates ofNeisseria meningitidis

Davidsen¹,

Amundsen²,

Rødland³

et al. 2007

FEMS Immunology &Amp; Medical Microbiology

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Neisseria meningitidis, or the meningococcus, is the source of significant morbidity and mortality in humans worldwide. Even though mutability has been linked to the occurrence of outbreaks of epidemic disease, meningococcal DNA repair pathways are poorly delineated. For the first time, a collection of meningococcal disease-associated isolates has been demonstrated to express constitutively the DNA glycosylases MutY and Fpg in vivo. DNA sequence analysis showed considerable variability in the deduced amino acid sequences of MutS and Fpg, while MutY and RecA were highly conserved. Interestingly, multi-locus sequence typing demonstrated a putative link between the pattern of amino acid substitutions and levels of spontaneous mutagenicity in meningococcal strains. These results provide a basis for further studies aimed at resolving the genotype/phenotype relationships of meningococcal genome variability and mutator activity.

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“…This observation is consistent with the findings by Naids and Rest (48), who showed that gonococci induce an oxidative response in neutrophils without generating O 2 Ϫ and H 2 O 2 in the extracellular milieu. Moreover, gonococci are not very sensitive to these primary products of the oxidative burst since the oxidative stress upregulates gonococcal catalase, which then rapidly detoxifies extracellularly released H 2 O 2 (3,62). Bactericidal ROS such as HOCl, however, is formed by MPO-dependent reactions in the phagolysosome.…”

Section: Discussionmentioning

confidence: 99%

Neisseria gonorrhoeae Porin Modifies the Oxidative Burst of Human Professional Phagocytes

et al. 2000

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A hallmark of infection with the gram-negative bacterium Neisseria gonorrhoeae is the local infiltration and subsequent activation of polymorphonuclear neutrophils. Several gonococcal outer membrane proteins are involved in the interaction with and the activation of these phagocytes, including gonococcal porin, the most abundant protein in the outer membrane. Previous work suggests that this porin plays a role in various cellular processes, including inhibiting neutrophils activation and phagosome maturation in professional phagocytes. Here we investigated the ability of porin to modify the oxidative metabolism of human peripheral blood neutrophils and monocytes in response to particulate stimuli (including live gonococci) and soluble agents. The activation of the oxidative metabolism was determined by chemiluminescence amplified with either luminol or lucigenin. We found that treatment of the phagocytes with porin inhibits the release of reactive oxygen species measured as luminol-enhanced chemiluminescence in response to zymosan, latex particles, and gonococci. The engulfment of these particles was not, however, affected by porin treatment. Similar effects of porin on the chemiluminescence response were observed in cytochalasin B-treated neutrophils exposed to the soluble chemotactic peptide N-formylmethionyl-leucyl-phenylalanine. This indicates that porin selectively inhibits granule fusion with those cellular membranes that are in direct contact with porin, namely, the phagosomal and plasma membranes. This porin-induced downregulation of oxidative metabolism may be a potent mechanism by which gonococci modulate oxygen-dependent reactions by activated phagocytes at inflammation sites.

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Variation in hydrogen peroxide sensitivity between different strains of Neisseria gonorrhoeae is dependent on factors in addition to catalase activity

Cited by 14 publications

References 18 publications

The protistan parasite Perkinsus marinus is resistant to selected reactive oxygen species

The protistan parasite Perkinsus marinus is resistant to selected reactive oxygen species

DNA repair profiles of disease-associated isolates ofNeisseria meningitidis

Neisseria gonorrhoeae Porin Modifies the Oxidative Burst of Human Professional Phagocytes

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