Variation in Separation Selectivity of Spongy Monoliths Caused by Hydrogen Bonding

Abstract

Spongy monoliths prepared using EVA, poly(ethylene-co-vinyl acetate) were chromatographically analyzed to reveal their bio-compatible properties. EVA formed as monolithic format including the hydrolyzed EVA (EVA-HYD) showed relatively hydrophobic properties as well as different separation selectivity for phenol and benzene diols compared with those on EDMA and GDMA polymer-based columns. Interestingly, planar recognition ability on EVA-HYD changed with higher content of acetonitrile in the mobile phase. IR study suggested that internal hydrogen bonding played as an important role on the chromatographic properties of EVA-HYD monoliths. EVA-HYD monoliths separated polypeptides based on hydrophobic interaction in acidic gradient mode, on the other hand, the recoveries of the polypeptides were almost quantitative in neutral mobile phase. As the results, EVA and EVA-HYD spongy monoliths changed the separation selectivity depending on mobile phase conditions.