Variation in the expression of Hsp27, αB-crystallin mRNA and protein in heart and liver of pigs exposed to different transport times

Zhang, Miao; Xin, Lingxiang; Bao, Endong; Hartung, J.; Yue, Zhenming

doi:10.1016/j.rvsc.2010.06.028

Cited by 8 publications

(7 citation statements)

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“…In contrast, the transcription of both hsp27 mRNA and αB-crystallin mRNA showed a dramatic and similar increase from the onset of heat stress (according to the results of qPCR). This suggests that sHsp expression was delayed or overtaxed due to rapid consumption of both sHsps at the onset of heat stress, since they are required for myocardial cell balance in response to stress [6]. It has been reported that overexpression of mutant αB-crystallin (R120G) in the heart can lead to cardiomyocyte death, dilation, and heart failure [50], suggesting that even molecular chaperones like Hsps need to be in a relatively stable state to play a protective role in vivo and in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…However, after the addition of myogenic factor, Hsp27 and αB-crystallin expression increases significantly [29]. This is probably linked to the function of these Hsps in the protection of cells against stress or to a specific function in a particular tissue [6], [24]. Hsp27 and αB-crystallin may modulate interaction between cellular factors by forming small or large oligomers, undergoing phosphorylation, and inducing cell–cell contact [51].…”

Section: Discussionmentioning

confidence: 99%

“…In particular, pathological changes in heart cells in response to stress have been investigated in transported pigs [6] as well as chickens [7]. For instance, a 60% death rate related to heart failure caused by transport stress was reported for pigs in Great Britain [8].…”

Section: Introductionmentioning

confidence: 99%

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Localization and Expression of Hsp27 and αB-Crystallin in Rat Primary Myocardial Cells during Heat Stress In Vitro

et al. 2013

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Neonatal rat primary myocardial cells were subjected to heat stress in vitro, as a model for investigating the distribution and expression of Hsp27 and αB-crystallin. After exposure to heat stress at 42°C for different durations, the activities of enzymes expressed during cell damage increased in the supernatant of the heat-stressed myocardial cells from 10 min, and the pathological lesions were characterized by karyopyknosis and acute degeneration. Thus, cell damage was induced at the onset of heat stress. Immunofluorescence analysis showed stronger positive signals for both Hsp27 and αB-crystallin from 10 min to 240 min of exposure compared to the control cells. According to the Western blotting results, during the 480 min of heat stress, no significant variation was found in Hsp27 and αB-crystallin expression; however, significant differences were found in the induction of their corresponding mRNAs. The expression of these small heat shock proteins (sHsps) was probably delayed or overtaxed due to the rapid consumption of sHsps in myocardial cells at the onset of heat stress. Our findings indicate that Hsp27 and αB-crystallin do play a role in the response of cardiac cells to heat stress, but the details of their function remain to be investigated.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Localization and Expression of Hsp27 and αB-Crystallin in Rat Primary Myocardial Cells during Heat Stress In Vitro

et al. 2013

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“…Biochemical and structural changes occur widely in the heart, liver, kidney and other organs of post-stressed animals [ 4 5 37 ]. Stress can induce specific pathological damage in the myocardium of heat-stressed broilers, as well as in transported pigs, and may even cause death [ 38 39 ]. Acute heart failure may be correlated with these stress phenomena.…”

Section: Introductionmentioning

confidence: 99%

The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells

et al. 2016

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The protective effect of aspirin during exposure to heat stress in broiler chickens was investigated. We assayed pathological damage, expression and distribution of Hsp90 protein and hsp90 mRNA expression in chicken heart tissues after oral administration of aspirin following exposure to high temperature for varying times. Heat stress induced increases in plasma aspartate aminotransferase, creatine kinase and lactate dehydrogenase activities while causing severe heart damage, which was characterized by granular and vacuolar degeneration, nuclear shrinkage and even myocardium fragmentation in cardiac muscle fibers. After aspirin administration, myocardial cells showed fewer pathological lesions than broilers treated with heat alone. A high positive Hsp90 signal was always detected in the nuclei of myocardial cells from broilers treated with aspirin, while in myocardial cells treated with heat alone, Hsp90 in the nuclei decreased, as did that in the cytoplasm. Aspirin induced rapid and significant synthesis of Hsp90 before and at the initial phase of heat stress, and significant expression of hsp90 mRNA was stimulated throughout the experiment when compared with cells exposed to heat stress alone. Thus, specific pre-induction of Hsp90 in cardiovascular tissue was useful for resisting heat stress damage because it produced stable damage-related enzymes and fewer pathologic changes.

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“…Studies of chronic heat stress have demonstrated altered physiologic, metabolic, biochemical, and cellular responses in animal models and poultry (Hu et al, 2007;Lu et al, 2007). Studies have also confirmed that the sudden death of mammals can occur as a result of stress-induced damage to heart tissue and myocardial cells (Yu et al, 2008;Zhang et al, 2011). The mechanisms leading to cellular damage and the death of animals subjected to such stresses remain poorly understood, although several factors and physiologic reactions have been investigated (Kamarck and Jennings, 1991).…”

Section: Introductionmentioning

confidence: 99%

Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo

Chen¹,

Zhang²,

Cheng³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro. After exposure to heat stress at 42°C for different durations, we observed a significant induction of CK, CK-MB, and LDH as well as pathologic lesions characterized by acute degeneration, suggesting that cell damage occurs from the onset of heat stress. Immunocytochemistry showed that Hsp70 was distributed mainly in the cytoplasm of myocardial cells in vivo and in vitro. Hsp70-positive signals in the cytoplasm were more prominent in intact areas than in degenerated areas after 60 min of heat stress. Hsp70 protein levels in myocardial cells in vitro decreased from the beginning to the end of heat stress. Hsp70 protein levels in rat heart 1995 Hsp70 in heat-stressed rat cardiac cells ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 1994-2005 tissues in vivo decreased gradually with prolonged heat stress, with a slight increase at the beginning of heat stress. These results indicate that Hsp70 plays a role in the response of cardiac cells to heat stress and that decreased Hsp70 levels are associated with damage to rat myocardial cells in vitro and in vivo. Significant differences were found in hsp70 mRNA, which began to increase after 20 min of heat stress in vitro and after 40 min in vivo. This indicates that hysteresis is involved in mRNA expression after heat stress in vivo.

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Variation in the expression of Hsp27, αB-crystallin mRNA and protein in heart and liver of pigs exposed to different transport times

Cited by 8 publications

References 49 publications

Localization and Expression of Hsp27 and αB-Crystallin in Rat Primary Myocardial Cells during Heat Stress In Vitro

Localization and Expression of Hsp27 and αB-Crystallin in Rat Primary Myocardial Cells during Heat Stress In Vitro

The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells

Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo

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