2020
DOI: 10.1093/jat/bkaa034
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Variation in the Relative Isomer Abundance of Synthetic and Biologically Derived Phosphatidylethanols and Its Consequences for Reliable Quantification

Abstract: Phosphatidylethanol (PEth) in human blood samples is a marker for alcohol usage. Typically, PEth is detected by reversed-phase liquid chromatography coupled with negative ion tandem mass spectrometry, investigating the fatty acyl anions released from the precursor ion upon collision-induced dissociation (CID). It has been established that in other classes of asymmetric glycerophospholipids, the unimolecular fragmentation upon CID is biased depending on the relative position (known as sn-position) of each fatty… Show more

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Cited by 24 publications
(20 citation statements)
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“…PC36:1 then consists of four sn-positional isomers:PC18:0/18:1, PC18:1/18:0, PC20:1/16:0, and PC16:0/20:1. Determination of the relative contributions of these four isomers to the overall PC36:1 ion signal would require generation of calibration curves similar those used with PC34:1, which is dependent on the availability, affordability, and purity69 of synthetic lipid standards. Nonetheless, the identification of multiple isomers represents an improvement over the sum-composition identification of PC34:1.…”
mentioning
confidence: 99%
“…PC36:1 then consists of four sn-positional isomers:PC18:0/18:1, PC18:1/18:0, PC20:1/16:0, and PC16:0/20:1. Determination of the relative contributions of these four isomers to the overall PC36:1 ion signal would require generation of calibration curves similar those used with PC34:1, which is dependent on the availability, affordability, and purity69 of synthetic lipid standards. Nonetheless, the identification of multiple isomers represents an improvement over the sum-composition identification of PC34:1.…”
mentioning
confidence: 99%
“…Quantitative accuracy is reliant on the calibrant standards being structurally identical to the analyte ( 35 ). To generate the calibration ( Figure 3B ), high regiopurity PC standards ( i.e.…”
Section: Resultsmentioning
confidence: 99%
“…Quantitative accuracy is reliant on the calibrant standards being structurally identical to the analyte (35). To generate the calibration (Figure 3B), high regiopurity PC standards (i.e., the sn-2 unsaturated PC 16:0/18:1n-7 and PC 16:0/18:1n-9) were analyzed as these sn-2 regioisomers are more commonly found in mammalian cells (typically above 90%) (16).…”
Section: Calibration Of Maldi-msi-ozid For Lipid Isomersmentioning
confidence: 99%
“…However, identification of sn -position via this method should be performed with caution, as the relative abundances of fragment ions formed from cleavage at the sn -1 and sn -2 positions can also depend on other factors, such as fatty acid chain length and degree of saturation. Additionally, mixtures of multiple positional isomers are likely representative of biological samples, further complicating this interpretation . Nonetheless, this methodology has been particularly useful in the imaging mass spectrometry analysis of PCs in rat brain tissue (Figure ).…”
Section: Changing the Ion Type After Ionizationmentioning
confidence: 99%
“…Additionally, mixtures of multiple positional isomers are likely representative of biological samples, further complicating this interpretation. 139 Nonetheless, this methodology has been particularly useful in the imaging mass spectrometry analysis of PCs in rat brain tissue (Figure 4). 140 While PC anions subjected to CID readily produce fragment ions indicative of the identity and sn-position of the fatty acyl chains in the lipid, 58 ionization suppression from more acidic endogenous glycerophospholipids generally prevents the detection of PC lipids directly from tissue in negative ion mode MALDI imaging mass spectrometry.…”
Section: ■ Changing the Ion Type After Ionizationmentioning
confidence: 99%