2018
DOI: 10.1016/j.jfca.2018.01.015
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Variation in tuber proximate composition, sugars, fatty acids and amino acids of eight Oromo dinich ( Plectranthus edulis ) landraces experimentally grown in Ethiopia

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Cited by 14 publications
(11 citation statements)
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“…The pH of the solution was adjusted to 2.2 with sodium hydroxide solution and further diluted to exactly 50 mL with 15% TCA. After centrifugation for 5 min at 3000 rpm, the supernatant was filtrated through a 0.45 µm nylon filter [106,107]. The solution contains primary and secondary amino acids that were further analyzed with the Ez:faast GC-MS kit (Phenomenex, Torrance, CA, USA).…”
Section: Gravimetric Parametersmentioning
confidence: 99%
See 1 more Smart Citation
“…The pH of the solution was adjusted to 2.2 with sodium hydroxide solution and further diluted to exactly 50 mL with 15% TCA. After centrifugation for 5 min at 3000 rpm, the supernatant was filtrated through a 0.45 µm nylon filter [106,107]. The solution contains primary and secondary amino acids that were further analyzed with the Ez:faast GC-MS kit (Phenomenex, Torrance, CA, USA).…”
Section: Gravimetric Parametersmentioning
confidence: 99%
“…The quadrupole measured the abundance of ions from 35 to 500 m/z. For the calibration, there were used solutions with amino acids mixture included in the kit mentioned above [107].…”
Section: Gravimetric Parametersmentioning
confidence: 99%
“…Free amino acids were analysed by mixing 3.5 ± 0.5 g of homogenized sample with 30 ml of 15% trichloroacetic acid (Gifty, De Meulenaer, & Olango, 2018). pH was adjusted to 2.2 and the extract was further diluted to exactly 50.0 ml with 15% trichloroacetic acid (TCA).…”
Section: Free Amino Acids Determinationmentioning
confidence: 99%
“…The quadrupole measured ion abundances from 35 to 500 m/z. Amino acid mixture solutions included in the kit were used for calibration [ 60 ].…”
Section: Methodsmentioning
confidence: 99%