Variation in Virulence for Mice and Rhesus Monkeys Among St. Louis Encephalitis Virus Strains of Different Origin

Monath, Thomas P.; Cropp, C B; Bowen, G. S.; Kemp, Graham E.; Mitchell, Carl J.; Gardner, J. J.

doi:10.4269/ajtmh.1980.29.948

Cited by 90 publications

(54 citation statements)

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“…Using Tl-oligonucleotide mapping of 57 SLE isolates, Trent et al (1981) classified them into three 'topotypes' representing different geographic areas of North America and different virus-vector relationships. Within a topotype there was significant covariation between genetic, epidemiological and biological markers (Monath et al, 1980;Monath, 1986). The apparent lack of systematic genetic differentiation seen in KUN may merely reflect the insensitivity of the method used here (which 'scans' approximately 4% of the genome for restriction sites) by comparison with the Tl-oligonucleotide fingerprinting procedure.…”

Section: Discussionmentioning

confidence: 97%

GENETIC ANALYSIS OF KUNJIN VIRUS ISOLATES USING HAEIII AND TAQI RESTRICTION DIGESTS OF SINGLE‐STRANDED cDNA TO VIRION RNA

Lobigs

Weir

Dalgarno

1986

Aust J Exp Biol Med

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Summary. The genotypic relatedness of 15 Kunjin (KUN) virus isolates from widely separated geographic regions in Australia was examined at the molecular level. Haelll and TaqI restriction digest profiles of cDNA transcribed from virion RNA revealed a close genetic similarity between all isolates. We estimate that the nucleotide sequence divergence between any pair of KUN . isolates is probably less than 1%. We conclude that a single KUN genetic type has existed in enzootic and epizootic areas of virus activity over an extended time span. The epidemiological implications of these results are discussed.

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Section: Discussionmentioning

confidence: 97%

GENETIC ANALYSIS OF KUNJIN VIRUS ISOLATES USING HAEIII AND TAQI RESTRICTION DIGESTS OF SINGLE‐STRANDED cDNA TO VIRION RNA

Lobigs

Weir

Dalgarno

1986

Aust J Exp Biol Med

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“…The survival of this strain in nature may be problematic unless it is mosquito-adapted, such as by prolonged transovarial transmission, or glycosylation is less important for attachment and penetration of mosquito cells. Different isolates of SLE virus vary significantly in their virulence for birds, mice and monkeys Bowen et al, 1980). The BeAn 246262 strain, which has only glycosylated E (Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular and biological characterization of a non-glycosylated isolate of St Louis encephalitis virus

Vorndam¹,

Mathews

Barrett

et al. 1993

Journal of General Virology

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The glycosylation patterns of the envelope (E) glycoprotein of several naturally occurring strains of St Louis encephalitis (SLE) virus were investigated. SLE viruses were found that contained both glycosylated and non-glycosylated E proteins, and one isolate (Tr 9464) that lacks N-linked glycosylation sites on its E protein was identified. SLE virus monoclonal antibodies that define E protein B cell epitopes and demonstrate biological activities reacted essentially to the same extent with glycosylated and non-glycosylated virions. These results indicate that glycosylation is not essential for epitope conformation or recognition. However, failure to glycosylate the E protein was associated with possible morphogenetic differences as manifested by reduced virus yields and differences in specific infectivity.

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“…Virulence assays were in Swiss outbred mice aged 21 days, following Monath et al (1980). Mice were divided into groups of five and inoculated either intracranially (i.c.)…”

Section: Passaging Of Mvementioning

confidence: 99%

Neurovirulence and neuroinvasiveness of Murray Valley encephalitis virus mutants selected by passage in a monkey kidney cell line

McMinn

Marshall

Dalgarno

1995

Journal of General Virology

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Variants of the prototype Murray Valley encephalitisvirus (MVE-1-51) were selected by serial plaque purification and amplification in monkey kidney (Vero) cells.Four clones (C1-C4) at passage levels two and nine (P2 and P9) were examined in 21-day-old Swiss outbred mice for neuroinvasiveness ( Genomic RNA of the cloned virus stocks was sequenced through the structural protein genes (E, prM/M and C) and the 5' untranslated region. Clone C2P2 was of high neuroinvasiveness whereas C2P9 was of low neuroinvasiveness; there were also decreased yields of C2P9 in C6/36 cells compared to C2P2 and MVE-1-51. These changes were associated with the substitution of valine for phenylalanine at amino acid position 141 of the C2P9 E protein. Clone C4P2 was of high neurovirulence and low neuroinvasiveness; C4P9 was of low neurovirulence, a change accompanied by a further reduction in neuroinvasiveness. Concomitantly, C4P9 showed a pronounced reduction in growth rates and yields in 21-day-old Swiss mouse brain, in mouse neuroblastoma cells and in C6/36 cells compared to parental virus. The phenotypic changes in clone 4 appear to be due to mutation(s) within non-structural protein genes.

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Variation in Virulence for Mice and Rhesus Monkeys Among St. Louis Encephalitis Virus Strains of Different Origin

Cited by 90 publications

References 0 publications

GENETIC ANALYSIS OF KUNJIN VIRUS ISOLATES USING HAEIII AND TAQI RESTRICTION DIGESTS OF SINGLE‐STRANDED cDNA TO VIRION RNA

GENETIC ANALYSIS OF KUNJIN VIRUS ISOLATES USING HAEIII AND TAQI RESTRICTION DIGESTS OF SINGLE‐STRANDED cDNA TO VIRION RNA

Molecular and biological characterization of a non-glycosylated isolate of St Louis encephalitis virus

Neurovirulence and neuroinvasiveness of Murray Valley encephalitis virus mutants selected by passage in a monkey kidney cell line

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