Variation of field symptoms and molecular diversity of the virus isolates associated with chilli leaf curl complex in different agroecological regions of Sri Lanka

Menike, G. D. N.; Costa, D. M. De

doi:10.4038/tar.v28i2.8192

Cited by 11 publications

(10 citation statements)

References 18 publications

(26 reference statements)

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“…The leaves become smaller and severely affected plants produce fewer and deformed fruits. Yield losses of 20–50% have been recorded in chilli [ 26 ], which may rise to 100% in the case of simultaneous infestation with thrips and mites [ 27 ]. Control options for ChiLCV and B. tabaci are very limited as insecticides continue to lose their efficacy due to the emergence of insecticide-resistant B. tabaci populations.…”

Section: Introductionmentioning

confidence: 99%

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Chakraborty

Ghosh

2022

Cells

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Chilli leaf curl virus (ChiLCV; genus: Begomovirus), transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in a persistent-circulative manner, is a major constraint in chilli production. The present study demonstrates for the first time that a topical spray of naked double-stranded RNA (dsRNA) on chilli plants causes mortality and inability to acquire and transmit ChiLCV in B. tabaci. dsRNA targeting heat shock protein 70 (hsp70) and fasciclin 2 (fas2) of B. tabaci Asia II 1 was first assessed under controlled conditions through oral delivery. Hsp70 and fas2 dsRNA resulted in up to 82.22% and 72% mortality of B. tabaci and around 12.4- and 8.5-fold decreases in mRNA levels, respectively, 24 h post-ingestion. ChiLCV copies in hsp70 dsRNA-fed B. tabaci steadily decreased with an increase in dsRNA concentration and were undetectable at a higher concentration of dsRNA. However, ChiLCV copies significantly increased in fas2 dsRNA-fed B. tabaci. Transmission of ChiLCV by B. tabaci was completely inhibited post-24 h feeding on hsp70 dsRNA at 3 μg/mL. Naked hsp70 dsRNA was topically sprayed on ChiLCV-infected chilli plants like an insecticide. 67.77% mortality of B. tabaci, 4.6-fold downregulation of hsp70 mRNA, and 1.34 × 1015-fold decreased ChiLCV copies in B. tabaci were recorded when adults were exposed to the dsRNA-treated plants under semi-field conditions. Foliar application of naked dsRNA reduced the ChiLCV transmission by 75% without any visible symptoms in the inoculated plants. A total of 2 consecutive sprays of dsRNA provided significant protection to B. tabaci for up to 20 days under semi-field conditions.

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Section: Introductionmentioning

confidence: 99%

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Chakraborty

Ghosh

2022

Cells

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“…To date, 445 begomovirus species have been reported 1 . One of the begomoviruses, i.e., chilli leaf curl virus (ChiLCV) is a major threat to chilli production in tropical and sub-tropical countries ( Senanayake et al, 2007 , 2012 ; Menike and De Costa, 2017 ; Oraonand and Tarafdar, 2018 ; Thakur et al, 2018 ). ChiLCV has caused several epidemics in India and Sri Lanka ( Senanayake et al, 2007 , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector

et al. 2022

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Bemisia tabaci (Hemiptera: Aleyrodidae) is a highly efficient vector in the spread of chilli leaf curl virus (ChiLCV, Begomovirus) which is a major constraint in the production of chilli in South Asia. Transcriptome analysis of B. tabaci post-6 h acquisition of ChiLCV showed differential expression of 80 (29 upregulated and 51 downregulated) genes. The maximum number of DEGs are categorized under the biological processes category followed by cellular components and molecular functions. KEGG analysis of DEGs showed that the genes are involved in the functions like metabolism, signaling pathways, cellular processes, and organismal systems. The expression of highly expressed 20 genes post-ChiLCV acquisition was validated in RT-qPCR. DEGs such as cytosolic carboxypeptidase 3, dual-specificity protein phosphatase 10, 15, dynein axonemal heavy chain 17, fasciclin 2, inhibin beta chain, replication factor A protein 1, and Tob1 were found enriched and favored the virus infection and circulation in B. tabaci. The present study provides an improved understanding of the networks of molecular interactions between B. tabaci and ChiLCV. The candidate genes of B. tabaci involved in ChiLCV transmission would be novel targets for the management of the B. tabaci-begomovirus complex.

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“…Among the plant pathogens, begomovirus which are vectored by the whitefly ( Bemisia tabaci ) cause serious crop losses to chilli and tomato. Chilli leaf curl virus , a begomovirus together with thrips and mite infestation, causes the chilli leaf curl complex, a serious biotic threat to chilli cultivation throughout the country 1,2 . In addition, Tobacco mosaic virus , Cucumber mosaic virus and a strain of Potato virus Y have been reported to cause virus diseases in chilli 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Chilli leaf curl virus, a begomovirus together with thrips and mite infestation, causes the chilli leaf curl complex, a serious biotic threat to chilli cultivation throughout the country. 1,2 In addition, Tobacco mosaic virus, Cucumber mosaic virus and a strain of Potato virus Y have been reported to cause virus diseases in chilli. 3 Tomato yellow leaf curl virus (a begomovirus) and Tomato spotted wilt virus (a tospovirus) have been reported as common viral infections of tomato.…”

Section: Introductionmentioning

confidence: 99%

Assessment of management practices, awareness on safe use of pesticides and perception on integrated management of pests and diseases of chilli and tomato grown by small‐scale farmers in selected districts of Sri Lanka

Costa

Weerathunga

et al. 2021

Pest Management Science

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BACKGROUND In this study, we surveyed through a structured and pilot‐tested questionnaire, the practices of pesticide usage by small‐scale (< 1.25 ha) chilli and tomato farmers from four districts of Sri Lanka and their perceptions on the effectiveness of pesticides and willingness to adopt integrated pest management (IPM). RESULTS We found significant (P < 0.05) variation among districts in farmer responses to 37 out of 59 survey questions. A majority of farmers were dependent on pesticides with only a minority practicing IPM. A majority perceived that their current pesticide usage was increasing, but was not excessive. A majority were aware of the negative impacts of pesticides on human health and environment. Farmer perception on the effectiveness of pesticides increased with age, but was not influenced by education level. Farmers having a higher opinion of pesticides made a greater number of applications during a cropping cycle. They also maintained a longer preharvest interval and did not use pesticide mixtures. A majority expressed willingness to initiate IPM, but identified lack of knowledge and technical knowhow on specific IPM practices for their crops as barriers to adoption and requested external support. Farmer willingness to adopt IPM is higher among older, more educated farmers and among full‐time farmers who are currently totally‐dependent on pesticides and whose major income source was farming. CONCLUSION We conclude that a significant extension effort in terms of farmer education on IPM and external assistance to develop the technological knowhow, which is tailor‐made to specific districts, is needed to facilitate adoption of IPM among these farmers.

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Variation of field symptoms and molecular diversity of the virus isolates associated with chilli leaf curl complex in different agroecological regions of Sri Lanka

Abstract: ABSTRACT. The present study was conducted to detect and identify the virus isolates and to determine the molecular diversity associated with CLCC-infected chilli plants grown in

Cited by 11 publications

References 18 publications

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector

Assessment of management practices, awareness on safe use of pesticides and perception on integrated management of pests and diseases of chilli and tomato grown by small‐scale farmers in selected districts of Sri Lanka

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