Variation of Polar Axes and Equatorial Diameters in Pollen Grains of Two Species of<i>Cassia</i>

Labouriau, M. L. Salgado; Vanzolini, P. E.; Melhem, Therezinha Sant'Anna

doi:10.1080/00173136509429142

Cited by 56 publications

(8 citation statements)

References 4 publications

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“…The arithmetic mean of equatorial diameter in polar view, apocolpium side, aperture length and width, exine thickness, and echinus dimensions are presented in Tables 2 and 3. For each specimen, a minimum of three permanent slides of acetolyzed pollen grains were analyzed to ensure sample standardization (Salgado-Labouriau et al 1965).…”

Section: Methodsmentioning

confidence: 99%

Palynotaxonomy of Calea sect. Meyeria (Asteraceae: Neurolaeneae)

Cartaxo-Pinto,

Vieira,

Nascimento

et al. 2023

Palynology

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Calea comprises 157 species that occur in the Neotropics. The genus is organized into eight subgenera and 18 sections. Calea sect. Meyeria consists of eight species occurring exclusively in south-central Brazil. In this study, pollen grains from these eight species were sampled from herborized flower buds in pre-anthesis. Pollen samples were acetolyzed, measured, and photographed under a light microscope. Unacetolyzed pollen grains were examined under a scanning electron microscope. The results were used to characterize pollen morphology and construct a palynotaxonomic key for Calea sect. Meyeria. The analyzed pollen grains were monads, isopolar, usually medium-sized, oblate spheroidal, tricolporate, with lalongate endoaperture, the sexine echinate and thicker than the nexine, and six spines in the apocolpium region. The eight species have very similar pollen grains, but some characters are informative for this section, namely pollen size, polar area, sexine ornamentation, and endoaperture characteristics. Despite the similarity of the pollen grains, palynological analysis was able to contribute to the delimitation of Calea species, providing new information for species distinction within Calea sect. Meyeria.

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Section: Methodsmentioning

confidence: 99%

Palynotaxonomy of Calea sect. Meyeria (Asteraceae: Neurolaeneae)

Cartaxo-Pinto,

Vieira,

Nascimento

et al. 2023

Palynology

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“…Measurements were performed within seven days of sample preparation by the acetolysis method in order to prevent alterations in pollen size, as reported by Salgado-Labouriau et al (1965), Melhem and Matos (1972), and Salgado-Labouriau (1973). The results are organized into tables (Tables 1–5).…”

Section: Methodsmentioning

confidence: 99%

“…Whenever possible, comparison specimens were analyzed for confirmation of the results (Gonc¸alves-Esteves et al 2020; Cartaxo-Pinto et al 2022a). Measurements were performed within seven days of sample preparation by the acetolysis method in order to prevent alterations in pollen size, as reported by Salgado-Labouriau et al (1965), Matos (1972), andSalgado-Labouriau (1973). The results are organized into tables (Tables 1-5).…”

Section: Measurement Of Pollen Grainsmentioning

confidence: 99%

Palynological diversity of some species of Coussareeae (Rubiaceae) from the Atlantic Forest, Brazil

et al. 2022

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Coussareeae is a neotropical tribe with a complex taxonomic history. Several authors considered it very close to the tribe Psychotrieae owing to similarities in their morphological characteristics, such as solitary ovules. The tribe Coussareeae constitutes the largest basal clade of the subfamily Rubioideae, and most of its genera are well represented in Brazil. The tribe is resolved into three suprageneric clades, including the Coccocypselum and Coussarea-Faramea clades, whose representatives are found in Brazil. This study aimed to ascertain whether pollen attributes corroborate the currently proposed phylogeny and systematics of the tribe. For this, pollen grains of 33 species belonging to the Coccocypselum and Coussarea-Faramea clades were analyzed. Specimens were obtained from herbaria, treated by the acetolysis method, measured, photographed, and described using light and scanning electron microscopy. Morphological analysis showed that pollen grains were medium (25-50 mm) or large (50-100 mm) in size; isopolar or apolar; 2-porate, 3-porate, or 3-colporate; and oblate spheroidal, suboblate, prolate spheroidal, or subprolate. Ornamentation varied among clade representatives. Multivariate analysis of quantitative data revealed that pollen diameter, apocolpium side, pore diameter, colpus length, and endoaperture width were the most significant characters. It can be seen from the results that the studied clades are formed by palynologically distinct genera in terms of polarity, aperture type, and ornamentation. Palynology confirms the proposed taxonomic classification.

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“…Equatorial diameter in polar view (EDPV) was measured for 25 pollen grains of one specimen (standard material) and, whenever possible, in 10 pollen grains of at least three additional specimens per species (comparison material). Polar diameter in equatorial view (PDEV), equatorial diameter in equatorial view (EDEV), polar area index (PAI), apocolpus side (AS), length and width of colpus/endoaperture, and exine thickness were measured for 10 pollen grains of a single specimen of each species; these measurements were taken randomly on a minimum of three slides (Salgado-Labouriau et al 1965).…”

Section: Measurement Of Pollen Grains Statistical Analysis and Termin...mentioning

confidence: 99%

Pollen morphology: a source of information for taxonomic studies within Campomanesia (Myrtaceae)

et al. 2022

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Campomanesia is a well-delimited Neotropical genus of Myrtaceae, but some of its species have a poorly understood circumscription. Pollen morphology can provide characters to clarify the relationships among such taxa. Here, we analyze the pollen grains of 16 species of Campomanesia using light and scanning electron microscopy. Heat map and multivariate regression tree analyses were performed aiming to detect differences among species based on pollen morphology. Pollen grains of the studied taxa are isopolar monads that are oblate or, less often, suboblate, 3(4)-colporate, syncolporate or parasyncolporate, with a triangular or quandrangular amb. The exine ornamentation varies among rugulate-granulate, granulate, rugulate and rugulate-espiculate. Fastigium is present in 14 species, and a costa is present in three species. The heat map and regression tree analyses based on 16 character states of pollen morphology were able to distinguish all species of Campomanesia studied here. Some species differ from all the other species, such as Campomanesia guaviroba due to its exclusively rugulate exine. Amb, shape and exine ornamentation were distinctive characters, as previously reported for other genera of Myrtaceae. However, the presence of fastigium also proved to be an informative pollen character for species distinction in Campomanesia. Based on the sample of this study, we can state that Campomanesia is a eurypalynous genus within Myrtaceae.

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Variation of Polar Axes and Equatorial Diameters in Pollen Grains of Two Species ofCassia

Cited by 56 publications

References 4 publications

Palynotaxonomy of Calea sect. Meyeria (Asteraceae: Neurolaeneae)

Palynotaxonomy of Calea sect. Meyeria (Asteraceae: Neurolaeneae)

Palynological diversity of some species of Coussareeae (Rubiaceae) from the Atlantic Forest, Brazil

Pollen morphology: a source of information for taxonomic studies within Campomanesia (Myrtaceae)

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