Variations in human corneal endothelial cell morphology and permeability to fluorescein with age

Carlson, Keith H.; Bourne, William M.; McLaren, Jay W.; Brubaker, Richard F.

doi:10.1016/0014-4835(88)90021-8

Cited by 149 publications

(91 citation statements)

References 14 publications

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“…The barrier function can be estimated from the endothelial permeability to fluorescein. 15 The efficiency of the pump can be studied by measuring the corneal deswelling rate from a 10% increase in thickness induced by 2 h of closed-eye aphakic soft contact lens wear. 16 The endothelial pump rate can be calculated from the deswelling rate and the endothelial permeability.…”

Section: Endothelium In Healthmentioning

confidence: 99%

Biology of the corneal endothelium in health and disease

Bourne

2003

Eye

260

198

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Section: Endothelium In Healthmentioning

confidence: 99%

Biology of the corneal endothelium in health and disease

Bourne

2003

Eye

260

198

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“…Age-related and post-operative changes, such as increasing pleomorphism, polymegethism, decreasing cell density in corneal endothelial layer and increasing corneal thickness, have already been observed and well described in several studies [5,7,[21][22][23][24][25][26]. Bourne [27] reported that the markedly enlarged endothelial cells in long-term corneal transplants have a reduced ability to keep cornea clear.…”

Section: Financial Interestmentioning

confidence: 99%

“…Several studies report a rate of 0.3-0.6 % endothelial cell loss per year in normal corneas [2,21,24,[28][29][30]. After intraocular surgery, the rate of endothelial cell loss is increased [2,31,32].…”

Section: Financial Interestmentioning

confidence: 99%

Assessment of endothelial cell density and corneal thickness in corneal grafts an average of 5 years after penetrating keratoplasty

Kettesy

Németh

Kemény-Beke

et al. 2014

Wien Klin Wochenschr

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SummaryBackground Corneal transparency is a useful indicator for corneal function. Our aim was to investigate central corneal endothelial cells and corneal thickness in transplanted corneas at an average of 5.4 years after penetrating keratoplastyPatients and methods The study involved 68 perforated keratoplasty patients with at least a 1 year followup. Post-operatively, the central corneal endothelial layer was observed using a contact specular microscope. Central endothelial cell density, corneal thickness and the coefficient of variation of endothelial size were statistically analysed.Results The post-operative follow-up time was ranging from 12 months to 23 years. Endothelial cell density (ECD) was 1,501 ± 249 cell/mm 2 . The average cell size was 673.6 ± 98.3 μm 2 , and the coefficient of variation of cell size was 0.61 ± 0.11. No difference in ECD was detected between diagnostic groups. Corneal thickness was 0.56 ± 0.06 mm. Correlation between ECD and post-operative time was not significant (r = 0.02; p = 0.85).Conclusion Our study concluded that ECD showed a higher rate of decrease after penetrating keratoplasty with no relation to pre-operative diagnosis.Keywords Endothelial cell loss · graft survival · penetrating keratoplasty · specular microscope Bestimmung der Endothelzelldichte und der Hornhautdicke in Hornhauttransplantaten circa 5 Jahre nach einer penetrierenden Keratoplastik Zusammenfassung Hintergrund Die Transparenz der Cornea ist ein nütz-licher Indikator der Funktion der Hornhaut. Das Ziel unserer Studie war es, die zentralen Endothelzellen der Cornea sowie die Dicke der Hornhaut in transplantierten Hornhäuten circa 5,4 (im Durchschnitt) Jahre nach penetrierender Keratoplastik zu untersuchen.Patienten und Methoden In die Studie wurden 68 Patienten nach perforierter Keratoplastik aufgenommen, die zumindest 1 Jahr nachuntersucht worden waren. Postoperativ wurde die zentrale Endothelschicht der Hornhautmittel seines Kontakt-Spekular Mikroskops untersucht. Die Dichte der zentralen Endothelzellen, die Dicke der Hornhaut und der Variations-Koeffizient der Größe des Endothels wurden statistisch analysiert.Ergebnisse Der Zeitpunkt der postoperativen Kontrollen variierte von 12 Monaten bis 23 Jahren. Die Endothelzelldichte (EZD) lag bei 1501 ± 249 Zelle/mm 2 . Der Mittelwert der Zellgröße war 673,6 ± 98,3 μm 2 und der Variationskoeffizient 0,61 ± 0,11. Es gab keinen Unterschied in der EZD zwischen den nach Diagnosen eingeteilten Gruppen. Die Korrelation zwischen der EZD und dem Zeitabstand zur Operation war nicht kant (r = 0,02; p = 0,85).Schlussfolgerung Unsere Studie zeigt unabhängig von der präoperativen Diagnose eine abfallende Tendenz der EZD nach penetrierender Keratoplastik.

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“…Endothelial cell populations decrease with age and lesions, resulting in a significantly larger size and rounded shape of individual cells and leading, finally, to fluid filtration into the stroma and increase in corneal thickness (CARLSON et al, 1988;GULLAPALLI et al, 1982;GWIN et al, 1982;WILSON & ROPER-HALL, 1982;LAULE et al, 1978;LAING et al, 1976). Therefore, there is a correlation between endothelial morphology and its functional capacity (LANDSHMAN et al, 1988;YEE et al, 1987;SCHULTZ et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural morphology and morphometry of the normal corneal endothelium of adult crossbred pig

et al. 2009

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Corneal endothelium constitutes a monolayer of polygonal cells. The integrity and health of this layer are essential for the maintenance of normal corneal transparency. This study reported by the first time in a detailed RESUMO O endotélio corneal é uma monocamada de células poligonais. A integridade e saúde dessa camada são essenciais para a manutenção da transparência corneal normal. Este estudo reportou pela primeira vez, de forma detalhada, a morfologia ultra-estrutural e a morfometria do endotélio corneal de suínos adultos mestiços à microscopia eletrônica de varredura (MEV

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Variations in human corneal endothelial cell morphology and permeability to fluorescein with age

Cited by 149 publications

References 14 publications

Biology of the corneal endothelium in health and disease

Biology of the corneal endothelium in health and disease

Assessment of endothelial cell density and corneal thickness in corneal grafts an average of 5 years after penetrating keratoplasty

Ultrastructural morphology and morphometry of the normal corneal endothelium of adult crossbred pig

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