Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM)

Lea, Peter J.; Temkin, Robert J.; Freeman, Karl B.; Mitchell, Grant A.; Robinson, Brain H.

doi:10.1002/jemt.1070270402

Cited by 44 publications

(30 citation statements)

References 17 publications

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“…Mitochondria from a variety of organisms and tissues have been described as dynamic organelles that change their shape and size and remodel their internal membranes or move to distinct cellular locations (11,36,54). These morphological transitions are greatly influenced by fusion and fission of the mitochondrial membranes and have been referred to as mitochondrial dynamics (29,46).…”

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confidence: 99%

Mitofusin-2 Maintains Mitochondrial Structure and Contributes to Stress-Induced Permeability Transition in Cardiac Myocytes

Papanicolaou

Khairallah

Ngoh

et al. 2011

Molecular and Cellular Biology

314

345

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Mitofusin-2 (Mfn-2) is a dynamin-like protein that is involved in the rearrangement of the outer mitochondrial membrane. Research using various experimental systems has shown that Mfn-2 is a mediator of mitochondrial fusion, an evolutionarily conserved process responsible for the surveillance of mitochondrial homeostasis. Here, we find that cardiac myocyte mitochondria lacking Mfn-2 are pleiomorphic and have the propensity to become enlarged. Consistent with an underlying mild mitochondrial dysfunction, Mfn-2-deficient mice display modest cardiac hypertrophy accompanied by slight functional deterioration. The absence of Mfn-2 is associated with a marked delay in mitochondrial permeability transition downstream of Ca 2؉ stimulation or due to local generation of reactive oxygen species (ROS). Consequently, Mfn-2-deficient adult cardiomyocytes are protected from a number of cell death-inducing stimuli and Mfn-2 knockout hearts display better recovery following reperfusion injury. We conclude that in cardiac myocytes, Mfn-2 controls mitochondrial morphogenesis and serves to predispose cells to mitochondrial permeability transition and to trigger cell death.

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confidence: 99%

Mitofusin-2 Maintains Mitochondrial Structure and Contributes to Stress-Induced Permeability Transition in Cardiac Myocytes

Papanicolaou

Khairallah

Ngoh

et al. 2011

Molecular and Cellular Biology

314

345

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“…The bundles of myofibrils were detected longitudinally and parallel, demonstrating the typical aspects such as studies reported by Peachey and Huxley (1962). The mitochondria show elongated and/or rounded shapes and we noted the mitochondrial crests according to report by Lea and Hollenberg (1989); Lea et al (1992Lea et al ( , 1994; Tanaka (1981Tanaka ( , 1989; Watanabe et al (1992).…”

Section: Discussionmentioning

confidence: 64%

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

Motoyama

Watanabe

Iyomasa

et al. 2009

Microscopy Res & Technique

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The nerve terminals of intrinsic muscular fibers of the tongue of adult wistar rats was studied by using silver impregnation techniques, transmission electron microscopy (TEM), and high resolution scanning electron microscopy (HRSEM) to observe the nerve fibers and their terminals. Silver impregnation was done according to Winkelman and Schmit, 1957. For TEM, small blocks were fixed in modified Karnovsky solution, postfixed in 1% buffered osmium tetroxide solution, and embedded in Spurr resin. For HRSEM, the parts were fixed in 2% osmium tetroxide solution with 1/15 M sodium phosphate buffer (pH 7.4) at 4 degrees C for 2 h, according to the technique described by Tanaka, 1989. Thick myelinated nerve bundles were histologically observed among the muscular fibers. The intrafusal nerve fiber presented a tortuous pathway with punctiform terminal axons in clusters contacting the surface of sarcolemma. Several myelinated nerve fibers involved by collagen fibers of the endoneurium were observed in HRSEM in three-dimensional aspects. The concentric lamellae of the myelin sheath and the axoplasm containing neurofilaments interspersed among the mitochondria were also noted. In TEM, myofibrils, mitochondria, rough endoplasmic reticulum, Golgi's apparatus, and glycogen granules were observed in sarcoplasm. It is also noted that the sarcomeres constituted by myofilaments with their A, I, and H bands and the electron dense Z lines. In areas adjacent to muscular fibers, there were myelinated and unmyelinated nerve fibers involved by endoneurium and perineurium. In the region of the neuromuscular junction, the contact with the sarcolemma of the muscular cell occurs forming several terminal buttons and showing numerous evaginations of the cell membrane. In the terminal button, mitochondria and numerous synaptic vesicles were observed.

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“…We have shown, in fact, that mitochondria from a large variety of human and animal organs, have mostly tubular cristae ( Figure 5, inset) and that lamellar cristae ( Figure 5, inset) are joined to the inner mitochondrial membrane by tubular connexions. Such tubular connexions (Figure 5), that were not seen by Lea et al (1994) who used the freezing cracking method, were documented by our technique since 1995 (Riva et al, 1995a;Riva et al, 1995b). They were named crista junctions using HVTEMT (Mannella et al, 1997, Perkins et al, 1997.…”

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confidence: 85%

The Fathers of Italian Histology

ISH¹

2009

Eur J Histochem

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Subscriptions, cancellations, business correspondence and any enquiries must be sent to the Tipografia PIME Editrice srl, Pavia, Italy. Cancellations must be received before the end of September to take effect at the end of the same year.No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means (electronic, electrostatic, magnetic type, mechanical, photocopying or otherwise) without written permission by the Publishers.Reg. Tribunale di Pavia n. 289/23.2.1984. Supported by the Ministero per i Beni e le Attività Culturali, Italy as a publication of high cultural value.Associato all'USPI Unione Stampa Periodica Italiana Disclaimer. Whilst every effort is made by the publishers and the editorial board to see that no inaccurate or misleading data, opinion or statement appears in this journal, they wish to make it clear that the data and opinions appearing in the articles or advertisements herein are the responsibility of the contributor or advisor concerned. Accordingly, the publisher, the editorial board and their respective employees, officers and agents accept no liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement. The Journal publishes original papers, technical reports, letters to the editor, review articles concerning investigations performed with the aid of biophysical, biochemical, molecular-biological, enzymatic, immunohistochemical, cytometric, and image analysis techniques. Areas of particular interest to the European Journal of Histochemistry include: -functional cell and tissue biology in animals and plants; -cell differentiation and death; -cell-cell interaction and molecular trafficking; -biology of cell development and senescence; -nerve and muscle cell biology; -cellular basis of diseases During this period, Carlo Rizzoli established the experimental approach for the study of the molecular basis of cell differentiation in vitro, anticipating some aspects of the present investigation on the potentiality of stem cells. Furthermore, Carlo Rizzoli was one of the first Italian scientists to publish its scientific reports in large-diffusion international journals, thus contributing to the world-wide diffusion of the seminal studies on the in vitro cell differentiation models. Editor-in-ChiefIn 1961, Carlo Rizzoli became Professor of Histology and general embryology and, since 1964 to 1999, Director of the Institute of Histology at the University of Bologna.The initial steps of this undertaking were challenging, since in 1963, following the recruitment of Oliviero Mario Olivo at the Chair of Human Anatomy, the facilities of the Institute of Histology were almost nonexistent. In few years, however, Carlo Rizzoli was able to organize an efficient research group of motivated young collaborators that included Paolo Carinci, Lia Guidotti, Francesco Antonio Manzoli, capable of introducing original and seminal lines of research into the national and international histological arena. In this way, a numb...

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Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM)

Cited by 44 publications

References 17 publications

Mitofusin-2 Maintains Mitochondrial Structure and Contributes to Stress-Induced Permeability Transition in Cardiac Myocytes

Mitofusin-2 Maintains Mitochondrial Structure and Contributes to Stress-Induced Permeability Transition in Cardiac Myocytes

Ultrastructure of motor nerve terminals in the anterior third of wistar rat tongue

The Fathers of Italian Histology

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