Variations in the lipid peroxidation and antioxidant biomarkers in some tissues of anadromous cyprinid fish during migration

Abstract

Alburnus tarichi is an endemic fish species inhabiting the Lake Van basin of Turkey. This anadromous cyprinid fish migrates for spawning to the freshwater inlets pouring into Lake Van, which has highly alkaline and brackish water. During the migration, the fish moves to a new habitat from a different habitat and encounters many challenges. The objective of the present study is to investigate whether antioxidant responses occur in the tissues of A. tarichi during its anadromous migration. To this end, fish were sampled at three different points and in two different periods from the migration route, including in Lake Van at prespawning, at the entrance of a freshwater stream (Karasu) at spawning and in the upstream of the freshwater stream at spawning. Malondialdehyde (MDA) content and antioxidant defenses including activities of the enzymes superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and catalase and the level of glutathione were assayed in liver, gill, white muscle, trunk kidney and anterior intestine tissues. Our results showed increased MDA levels in liver, white muscle and anterior intestine and tissue-specific antioxidant responses in the freshwater environment. This study reports that alterations occurred in the antioxidant defense system indicators in the tissues of anadromous cyprinid fish during migration and that the antioxidant defenses might reflect an important role in spawning migration that ultimately leads to accomplishment of reproductive activity.

Histochemical changes, cellular dynamics and antioxidant responses in the corpuscles of Stannius of the female Lake Van fish ( Alburnus tarichi Güldenstädt, 1814) (Cyprinidae) during migration from highly alkaline and brackish water to freshwater

“…Van fish during spawning migration were also determined in our previous investigation (Kaptaner & Dogan, 2019). In line with the results of the present study, Arihan et al (2017) reported an increase in erythrocyte osmotic fragility in Lake Van fish caught from freshwater during spawning period, implying that the fish lives a physiological stress in freshwater causally related with physicochemical changes in ambient water.…”

“…On the other hand, many studies performed in different fishes and aquatic organisms stated that changes in abiotic factors such as salinity, pH, oxygen levels and temperature, as well as osmoregulatory processes and metabolic activities may have an effect on tissue antioxidant defences, including abovementioned indicators (Wilhelm Filho et al, 2001; Martinez‐Alvarez et al, 2002; Lushchak, 2011). Variations of oxidative stress biomarkers in the different tissues of anadromous Lake Van fish during spawning migration were also determined in our previous investigation (Kaptaner & Dogan, 2019). In line with the results of the present study, Arihan et al (2017) reported an increase in erythrocyte osmotic fragility in Lake Van fish caught from freshwater during spawning period, implying that the fish lives a physiological stress in freshwater causally related with physicochemical changes in ambient water.…”

Apoptosis and cell proliferation in the epithelia of the esophagus and intestine ofAlburnus tarichiGüldenstädt, 1814 (Cyprinidae) during migration from highly alkaline and brackish water to fresh water

“…As a result of osmoregulatory processes, elevated tissue lipid peroxidation or oxidative stress and significant physiological alterations have been reported in Acipenser naccarii (Martinez-Alvarez et al 2002). In our previous studies conducted on Lake Van fish, we observed increased erythrocyte osmotic fragility causally related to physicochemical changes during migration to fresh water, that indicated physiological stress (Arihan et al 2017), and lipid peroxidation, an indicator of oxidative stress, in the anterior intestine of fish sampled from a freshwater environment (Kaptaner & Dogan 2019). Apart from the above-mentioned impact, the feeding status and diet type also alter epithelial cell proliferation and apoptosis in fish (Sirri et al 2014;Takahashi et al 2014).…”

Carga parasitária x estresse oxidativo em Satanoperca jurupari (Heckel, 1840) na Amazônia Oriental

“…Assim, níveis elevados de SRAT são característicos de organismos em situação de estresse oxidativo (GASCHLER et al, 2017 LACAZE et al, 2019). Todavia, estudos de correlação dos impactos das infecções parasitárias na geração de estresse oxidativo são recentes e as referências ainda são limitadas, a maioria dos relatos em peixes é com foco em aspectos ecológicos, pesticidas ou compostos inorgânicos (PAITAL et al, 2019;SRIVASTAVA et al, 2017;KAPTANER et al, 2019).…”