Varicosity-Schwann Cell Interactions Mediated by ATP in the Mouse Vas Deferens

Lin, Yong; Bennett, Maxwell R.

doi:10.1152/jn.00772.2004

Cited by 6 publications

(9 citation statements)

References 42 publications

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“…A similar conclusion was reached for a population of cells present on the adventitia of large intrarenal arteries. On the other hand, an EGFP-positive cell population was found within the perivascular space that concurrently expressed protein S100B and exhibited morphological characteristics of Sc (15,27,28,39). Similarly, observation of the pelvic mucosa revealed a meshwork of EGFP-and protein S100B-positive cells with anatomic characteristics of Sc.…”

Section: Discussionmentioning

confidence: 94%

“…Although protein S100B is a classic marker of Sc (11,13,20,25,27,32,39,43), this protein is expressed by both fully differentiated myelinated and unmyelinated Sc (5,11,22,35). Hence, the presently used protein S100B/EGFP transgenic mice do not theoretically allow one to distinguish myelinated from unmyelinated Sc.…”

Section: Discussionmentioning

confidence: 99%

“…1, merged panel). On pure anatomic grounds (15,25,27,28,39), these cells likely represent the Sc population located within the renal cortex. Figure 2 illustrates at a higher power and spatial definition the phenotypic traits of these Sc.…”

Section: Animalsmentioning

confidence: 99%

“…To validate EGFP as a reporter gene, we assessed which of these EGFP-positive cell types simultaneously expressed immunoreactive protein S100B, a "classic" marker protein of Sc (13,25,27,32,39,43). As illustrated in the portion of the renal cortex shown in Fig.…”

Section: Renal Distribution Of Egfp-expressing Cells and Anatomic Idementioning

confidence: 99%

“…This mouse strain has thus far exclusively been used in studies of the central nervous system (17, 46). Many in vivo or in vitro studies on Sc associated with peripheral nerves have relied on protein S100 as a cell marker (13,25,27,32,39,43). In fact, protein S100 is recognized as a recurrently expressed cell factor along the Sc lineage from precursor/immature to both myelinated or unmyelinated Sc (5,11,22,25,35).…”

mentioning

confidence: 99%

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Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice

Darlot¹,

Artuso²,

Lautrédou-Audouy³

et al. 2008

American Journal of Physiology-Renal Physiology

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Darlot F, Artuso A, Lautredou-Audouy N, Casellas D. Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice. Am J Physiol Renal Physiol 295: F1142-F1148, 2008. First published August 20, 2008 doi:10.1152/ajprenal.00599.2007, associated axons, and nearby vascular endothelium constitute a functional trilogy of major importance during the development and regrowth of peripheral vascular nerves. The goal of the present study is to provide a technique of triple fluorescence confocal imaging of these cell types along renal preglomerular vessels. We took advantage of a protein S100B/EGFP transgenic mouse to visualize Sc. The endothelium was labeled with an intravenous injection of fluorescently tagged lectin, and after tissue processing, adrenergic nerves were revealed with an antibody against the marker protein synaptophysin. As a validation step, we found that EGFPpositive perivascular cells with prominent cell bodies and extensive, multidirectional cell processes were protein S100B positive. They were identified as Sc and indirectly assumed to be unmyelinated Sc. By contrast, we found strong EGFP expression in proximal epithelial cells and in the epithelium lining thin limbs of Henle. This epithelial fluorescence was not associated with immunoreactive protein S100B and thus corresponded to ectopic EGFP expressions in this mouse strain. Sc were organized in bundles or as a meshwork surrounding the preglomerular vasculature from arcuate arteries to afferent arterioles. No Sc were detected in the medulla. Although most Sc were closely apposed to adrenergic varicosities, many varicosities were not associated with detectable Sc processes. The present technique, and the capacity of confocal microscopy to yield three-dimensional imaging, allow the study of the microtopology of Sc and related sympathetic axons in the renal perivascular interstitium.immunohistochemistry; triple fluorescence labeling; synaptophysin; Griffonia simplicifolia; isolectin; myelinated and unmyelinated nerves RECENT STUDIES UNDERLINE THE crucial role played by Schwann cells (Sc) in the development and maturation of peripheral sympathetic nerves (5, 21). Functional interactions between Sc and axons are also present during posttraumatic axonal regrowth (5, 7). In this respect, the addition and/or migration of Sc within artificial nerve guides appears as an essential determinant of axonal regrowth (13,26,43). In addition, recent studies establish the existence of common molecular mechanisms that link axonal and vascular guidance during early development (1,31,33,34,38), during posttraumatic axonal regrowth (20), and during tumoral angiogenesis (4, 34). The existence of such a "functional trilogy" among Sc, axons, and vascular endothelial cells gives relevance and importance to the possibility of specifically, and simultaneously, imaging these three cell types in normal and pathological animal models.Transmission electron microscopy (TEM) and immunohistochemist...

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Renal Distribution Of Egfp-expressing Cells and Anatomic Idementioning

confidence: 99%

mentioning

confidence: 99%

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Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice

Darlot¹,

Artuso²,

Lautrédou-Audouy³

et al. 2008

American Journal of Physiology-Renal Physiology

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Peripheral Nervous System

Burnstock¹,

Verkhratsky

2012

Purinergic Signalling and the Nervous System

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Schwann cells in rat vascular autonomic nerves activated via purinergic receptors

Lin

Bennett²

2006

NeuroReport

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Schwann cells at the somatic neuromuscular junction possess adenosine receptors that when activated by the release of endogenous transmitter modulate quantal transmitter release. Recently, purinergic receptors have been shown to exist on Schwann cells of axon varicosities in visceral smooth muscle where they are activated by endogenous transmitters to give a calcium transient, although adenosine receptors were not identified. In the present work, we show that Schwann cells associated with axon varicosities of vascular smooth muscle, namely that of mesenteric blood vessels, possess both adenosine and purinergic receptors that when activated give rise to calcium transients in these cells. Then qualitative differences exist in the extent of adenosine and purine receptors that give rise to calcium transients in Schwann cells located in these different muscles.

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Varicosity-Schwann Cell Interactions Mediated by ATP in the Mouse Vas Deferens

Cited by 6 publications

References 42 publications

Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice

Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice

Peripheral Nervous System

Schwann cells in rat vascular autonomic nerves activated via purinergic receptors

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