Varietal Differences in Plant Regeneration from Solid and Suspension Cultures in Onion (Allium cepa L.).

Tanikawa, Tsuyoshi; Takagi, Motoki; Ichii, Masahiko

doi:10.2503/jjshs.67.856

Cited by 6 publications

(7 citation statements)

References 13 publications

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“…The percentage of calli that regenerated shoots among the calli transferred onto RE medium was 34% for the embryogenic calli and 58% for the LL type calli. These regeneration frequencies were comparable with a previous report by Tanikawa et al (1998), who tested 22 onion cultivars and obtained an average regeneration frequency of 43.6% for the embryogenic calli and 60.5% for the LL type calli. Thus, we considered that these calli were suitable for transformation experiments.…”

Section: Discussionsupporting

confidence: 79%

“…We preliminarily examined the frequency of plant regeneration from both radiclederived embryogenic calli and proliferated LL type calli in suspension cultures prepared by the methods of Tanikawa et al (1998). The percentage of calli that regenerated shoots among the calli transferred onto RE medium was 34% for the embryogenic calli and 58% for the LL type calli.…”

Section: Discussionmentioning

confidence: 99%

“…of the calli amenable to Agrobacterium-mediated transformation Embryogenic calli were induced from the radicles of germinated onion seeds on SCC medium and vigorously growing light yellow and large (LL) type calli were proliferated by suspension cultures of the embryogenic calli in LCC medium as described by Tanikawa et al (1998). Embryogenic calli and LL type calli were then cocultured with Agrobacterium and transient GFP expression was examined 1-6 days after coculture.…”

Section: Distinctionmentioning

confidence: 99%

“…Senshu-Chu-Koudakaki) was used in transformation experiments. Callus induction and propagation of light yellow and large (LL) type calli via a suspension culture of onion were performed as described by Tanikawa et al (1998). Media used for tissue culture are listed in Table 1.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The procedure was successfully applied to 22 cultivars of onion (Tanikawa et al 1998). However, no successful Agrobacterium-mediated transformation was observed, using the calli prepared according to their method.…”

mentioning

confidence: 99%

See 4 more Smart Citations

A novel autofluorescence-based selection of calli amenable to Agrobacterium-mediated transformation in onion (Allium cepa L.)

Kamata¹,

Masamura²,

Miyazaki³

et al. 2011

Plant Biotechnology

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Distinctionmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A novel autofluorescence-based selection of calli amenable to Agrobacterium-mediated transformation in onion (Allium cepa L.)

Kamata¹,

Masamura²,

Miyazaki³

et al. 2011

Plant Biotechnology

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Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

et al. 2015

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The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus induction. Scanning electron micrograph (SEM) analysis of embryogenic callus showed all stages of somatic embryos such as globular, scuetellar and coleoptilar. Plant regeneration was significantly affected by the composition of embryogenic callus induction medium. The greatest frequency of somatic embryo conversion was obtained from embryogenic callus developed in MS medium with 2,4-D (70.1%) followed by picloram (38.9%) and 2,4-D + picloram (34.5%). The germinated plantlets were further developed on the half-strength MS medium containing 3% sucrose and were acclimatized in the culture room with 98% survival rate.

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Towards genetic transformation of local onion varieties

Marinangeli

Deluchi

Ríos

et al. 2006

Electron. J. Biotechnol.

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Varietal Differences in Plant Regeneration from Solid and Suspension Cultures in Onion (Allium cepa L.).

Cited by 6 publications

References 13 publications

A novel autofluorescence-based selection of calli amenable to Agrobacterium-mediated transformation in onion (Allium cepa L.)

A novel autofluorescence-based selection of calli amenable to Agrobacterium-mediated transformation in onion (Allium cepa L.)

Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

Towards genetic transformation of local onion varieties

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