Vascular endothelial dysfunction associated with elevated serum homocysteine levels in rat adjuvant arthritis: effect of vitamin E administration

Can, Cenk; Çınar, Mustafa; Koşay, Sezen; Evinç, Akgün

doi:10.1016/s0024-3205(02)01700-9

Cited by 15 publications

(18 citation statements)

References 30 publications

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“…In the present study, we investigated endothelial function in the AIA model, which is commonly accepted as having many histologic and clinical features in common with human RA (31) and which is widely used to predict clinical efficacy of new therapies in RA (32). In accordance with previous studies (12)(13)(14)(15)(16)(33)(34)(35), our data show that endothelial function assessed by the vasodilating response to ACh is impaired in AIA rats. We also demonstrated that endothelial dysfunction correlates with disease activity.…”

Section: Discussionsupporting

confidence: 80%

“…In the model of adjuvant-induced arthritis (AIA), data demonstrated that vessels from AIA rats overproduced superoxide anions (O 2 Ϫ ) (12)(13)(14) and that BH 4 supplementation decreased endothelial dysfunction (12), suggesting that the deficiency in BH 4 may contribute to the uncoupling of eNOS and subsequent production of O 2 Ϫ . However, treatment with vitamin E used as an antioxidant both improved (15) and decreased (16) endothelial function in AIA rats. To our knowledge, it is currently not known whether a deficit in NO availability accounts for arthritis-associated endothelial dysfunction.…”

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confidence: 92%

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Endothelial dysfunction in rat adjuvant‐induced arthritis: Up‐regulation of the vascular arginase pathway

Prati¹,

Berthelot²,

Wendling³

et al. 2011

Arthritis & Rheumatism

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Objective. To investigate whether arginase pathway abnormalities occur in vessels from rats with adjuvant-induced arthritis (AIA), and to determine whether the up-regulation of arginase, which reciprocally regulates nitric oxide synthase (NOS) by competing for the same substrate, L-arginine, contributes to endothelial dysfunction in AIA.Methods. We performed vascular reactivity experiments on thoracic aortic rings from AIA rats and control rats, and we investigated the response of rings to norepinephrine (NE), sodium nitroprusside (SNP), and acetylcholine (ACh). ACh-induced relaxation was evaluated in the presence (or not in the presence) of the NOS inhibitor N G -nitro-L-arginine methyl ester (L-NAME), the arginase inhibitor N -hydroxy-nor-Larginine (nor-NOHA), or both. Aortic arginase activity was measured using a spectrophotometric method, and the expression of arginase and endothelial NOS (eNOS) was evaluated by Western blotting.Results. ACh-induced vasodilation was significantly impaired in AIA rats, while the responses to NE and to SNP did not differ from those in control rats. L-NAME reduced ACh-induced vasodilation to a lesser extent in AIA rats than in control rats. Incubation of aortic rings with nor-NOHA enhanced the vascular response to ACh in AIA rats and reversed the effects of L-NAME. Compared with control rats, AIA rats exhibited increased vascular expression of arginase II (by 22%) (P < 0.05) as well as increased arginase activity (by 49%) (P < 0.05), whereas eNOS expression was unchanged. Finally, arginase activity and expression correlated positively with arthritis severity.Conclusion. Our results are consistent with the notion that arginase up-regulation plays a role in AIAassociated endothelial dysfunction. They suggest that arginase might be an attractive new target for treating endothelial dysfunction in arthritis.

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Section: Discussionsupporting

confidence: 80%

mentioning

confidence: 92%

Endothelial dysfunction in rat adjuvant‐induced arthritis: Up‐regulation of the vascular arginase pathway

Prati¹,

Berthelot²,

Wendling³

et al. 2011

Arthritis & Rheumatism

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“…[20][21][22] Furthermore, it was reported that free radicals play an important role in endothelial dysfunction in AIA rats. 22) Drugs such as vitamin E and tetrahydrobiopterin, 21,22) which have no anti-inflammatory effect, improve endothelial dysfunction in AIA rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of Keishibukuryogan on Vascular Function in Adjuvant-Induced Arthritis Rats

Nozaki

Goto

Nakagawa

et al. 2007

Biological & Pharmaceutical Bulletin

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“…Rheumatoid arthritis (RA) is a very common autoimmune disease which affects 1-1.5% of the world population, with high prevalence in women 1,2 . nevertheless, it can occur at any age, with more frequent beginning in adults aged 40-60 years 1 .…”

Section: Introductionmentioning

confidence: 99%

“…Research conducted with RA patients is difficult to be suitably conducted due to the exhaustive tests which are repeated and by the ingestion of medication or daily habits which may interfere in the inflammatory process and response to exercise. Thus, in vivo studies using arthritis experimental models may provide useful information 2,10,11 . nevertheless, a problem associated with this approach may be the stress induced to the animals, since they are made to perform tasks related to physical activity.…”

Section: Introductionmentioning

confidence: 99%

Evidências de que um protocolo de atividade física pode reduzir a contagem de leucócitos sinoviais de ratos artríticos

Gomes

Bressan

Silva

et al. 2013

Rev Bras Med Esporte

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ABSTRACTintroduction: physical activity is thought to be beneficial to arthritis, delaying disability and/or improving joint function. in vivo studies using experimental models of arthritis may provide useful information about such benefits. Objective: The aim of the present study was to evaluate the effects of the low-intensity exercise on a model of CFA-induced arthritis in rats. Methods: Articular incapacitation was measured by the paw elevation time in 1-min periods of stimulated walk. Edema was evaluated by the knee-joint diameter. Synovial exudate was sampled after 10 days for leukocyte count. The exercise protocol consisted of a 2-min period of stimulated walk in the 1st day, 10 min in the 2nd day, and 20 min from the 3rd to the 10th day; The control animals were submitted to 1-min period of stimulated walk once a day over 10 days. Corticosteroid involvement was assessed by treating the animals with aminoglutethimide. Results: The exercise protocol produced a slight but sustained reduction of disability and joint swelling associated with a large reduction in the synovial leukocyte count. Aminoglutethimide only prevented the effect on synovial leukocyte count. Conclusion: These results suggest that a low-intensity physical activity does not aggravate the symptoms of arthritic animals, in fact showing a slight improvement, and still can markedly reduce the migration of leukocytes into the synovial space.

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Vascular endothelial dysfunction associated with elevated serum homocysteine levels in rat adjuvant arthritis: effect of vitamin E administration

Cited by 15 publications

References 30 publications

Endothelial dysfunction in rat adjuvant‐induced arthritis: Up‐regulation of the vascular arginase pathway

Endothelial dysfunction in rat adjuvant‐induced arthritis: Up‐regulation of the vascular arginase pathway

Effects of Keishibukuryogan on Vascular Function in Adjuvant-Induced Arthritis Rats

Evidências de que um protocolo de atividade física pode reduzir a contagem de leucócitos sinoviais de ratos artríticos

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