Vascular endothelial growth factor augments the healing of demineralized bone matrix grafts

Emad, Bassem; Sherif, El-Mofty; Basma, Gamal M.; Wong, Ricky W.K.; Bendeus, Margareta; Rabie, A. Bakr M.

doi:10.1016/j.ijsu.2006.04.005

Cited by 24 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because PEDF is known as a potent antiangiogenic factor its expression in bone would have undesirable consequences. Previous reports showed that VEGF can have a direct effect on osteoblast differentiation and bone mineralization [19,20,26]. VEGF plays important role in fracture healing through coupled angiogenesis and osteogenesis.…”

Section: Discussionmentioning

confidence: 99%

Pigment epithelium derived factor upregulates expression of vascular endothelial growth factor by human mesenchymal stem cells: Possible role in PEDF regulated matrix mineralization

Tombran-Tink

Niyibizi

2016

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Pigment epithelium-derived factor (PEDF) encoded by serpinf1 is a potent anti-angiogenic factor found in a wide variety of fetal and adult tissues. Several reports have shown that lack of PEDF leads to osteogenesis imperfecta (OI) type VI whose hallmark is a defect in mineralization that leads to excessive osteoid build up that fails to mineralize. Because PEDF is antiangiogenic factor it would pose serious consequences on bone development and healing of fractures. To understand possible mechanisms by which PEDF plays a role in bone development and regulation of matrix mineralization, we determined the effects of exogenous PEDF on vascular endothelial growth factor (VEGF) expression by human mesenchymal stem cells (hMSCs) and mechanisms of its regulation by PEDF. Human MSCs incubated in normal medium supplemented with exogenous PEDF increased VEGF expression; this increase was also seen when PEDF was added to hMSCs undergoing osteogenic differentiation. MSCs maintained in osteogenic medium increased synthesis of both VEGF and PEDF but both factors were maintained relatively in balance during differentiation. To understand mechanisms by which exogenous PEDF regulated VEGF expression, hMSCs exposed to PEDF activated Erk signaling pathway in MSCs; inhibition of Erk signaling reduced VEGF mRNA expression as well as protein production suggesting that PEDF regulates VEGF expression in MSCs via Erk signaling pathway. In conclusion, PEDF increases VEGF expression by MSCs suggesting that regulation of VEGF by PEDF may be part of the mechanisms by which PEDF regulates osteoblastic mineralization.

show abstract

Section: Discussionmentioning

confidence: 99%

Pigment epithelium derived factor upregulates expression of vascular endothelial growth factor by human mesenchymal stem cells: Possible role in PEDF regulated matrix mineralization

Tombran-Tink

Niyibizi

2016

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…VEGF is a known key mediator of angiogenesis and has roles in both bone-forming processes -direct (intramembranous) and indirect (endochondral ossification) (Emad et al 2006, Dai & Rabie 2007, Li et al 2009). Highlighting VEGF's essential role during bony repair, Street et al (2002) found that in a mouse bone fracture model, the absence of VEGF delayed bone formation by halting the initial soft callus from being converted into hard bony callus.…”

Section: Potential Changes At the Adjacent Non-injured Region Of The mentioning

confidence: 99%

RECENT RESEARCH ON THE GROWTH PLATE: Mechanisms for growth plate injury repair and potential cell-based therapies for regeneration

Chung

Chen

2014

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Injuries to the growth plate cartilage often lead to bony repair, resulting in bone growth defects such as limb length discrepancy and angulation deformity in children. Currently utilised corrective surgeries are highly invasive and limited in their effectiveness, and there are no known biological therapies to induce cartilage regeneration and prevent the undesirable bony repair. In the last 2 decades, studies have investigated the cellular and molecular events that lead to bony repair at the injured growth plate including the identification of the four phases of injury repair responses (inflammatory, fibrogenic, osteogenic and remodelling), the important role of inflammatory cytokine tumour necrosis factor alpha in regulating downstream repair responses, the role of chemotactic and mitogenic platelet-derived growth factor in the fibrogenic response, the involvement and roles of bone morphogenic protein and Wnt/B-catenin signalling pathways, as well as vascular endothelial growth factor-based angiogenesis during the osteogenic response. These new findings could potentially lead to identification of new targets for developing a future biological therapy. In addition, recent advances in cartilage tissue engineering highlight the promising potential for utilising multipotent mesenchymal stem cells (MSCs) for inducing regeneration of injured growth plate cartilage. This review aims to summarise current understanding of the mechanisms for growth plate injury repair and discuss some progress, potential and challenges of MSC-based therapies to induce growth plate cartilage regeneration in combination with chemotactic and chondrogenic growth factors and supporting scaffolds.

show abstract

“…Intramembranous bone formation also depends on VEGF signaling. Blocking the VEGF receptors, VEGFR1 and VEGFR2, with neutralizing antibodies decreased blood vessel formation and bone regeneration (15), while administering exogenous VEGF increased formation of mineralized bone within bone defects (14,16,17). In addition, VEGF regulates maturation and differentiation of osteoclasts and, thus, is necessary for maintenance of normal bone remodeling (14,18,19).…”

Section: Introductionmentioning

confidence: 99%

Osteoblast-derived VEGF regulates osteoblast differentiation and bone formation during bone repair

Hu¹,

Olsen²

2016

Journal of Clinical Investigation

519

396

View full text Add to dashboard Cite

Vascular endothelial growth factor augments the healing of demineralized bone matrix grafts

Abstract: VEGF+DBM(IM) powder is a promising graft material, and warrants further clinical investigation.

Cited by 24 publications

References 22 publications

Pigment epithelium derived factor upregulates expression of vascular endothelial growth factor by human mesenchymal stem cells: Possible role in PEDF regulated matrix mineralization

Pigment epithelium derived factor upregulates expression of vascular endothelial growth factor by human mesenchymal stem cells: Possible role in PEDF regulated matrix mineralization

RECENT RESEARCH ON THE GROWTH PLATE: Mechanisms for growth plate injury repair and potential cell-based therapies for regeneration

Osteoblast-derived VEGF regulates osteoblast differentiation and bone formation during bone repair

Contact Info

Product

Resources

About