Vascular endothelial growth factor gene polymorphisms are associated with the risk of developing adenomyosis

Abstract

Vascular endothelial growth factor (VEGF), a major mediator of angiogenesis and vascular permeability, may play a key role in the development of adenomyosis. The aim of this study was to investigate whether these four VEGF polymorphisms (-2578C/A, -1154G/A, -460C/T, and +936C/T) were associated with the risk of adenomyosis development. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in 174 adenomyosis patients and 199 frequency-matched control women. There were significant differences between patients and control group in allele frequencies and genotype distributions of the -2578C/A polymorphisms (P = 0.010 and 0.044, respectively). Compared with the C/C genotype, the A/A + C/A genotype could significantly modify the risk of developing adenomyosis [odds ratio (OR) = 0.64, 95% confidence interval (CI) = 0.42-0.97]. For the -1154G/A polymorphism, the allele frequencies and genotype distributions in patient group were significant different from those of the controls (P = 0.001 and 0.007, respectively). Compared with the G/G genotype, the A/A + G/A genotype could significantly decrease the risk of developing adenomyosis (OR = 0.51, 95% CI = 0.33-0.80). However, the genotype distributions and allele frequencies of the -460C/T and +936C/T polymorphisms did not significantly differ between controls and patients (all P value > 0.05). The haplotype analysis suggested that the TGA (VEGF -460/-1154/-2578) and CGA haplotypes exhibited a significant decrease in the risk of developing adenomyosis compared with the haplotype of TGC (OR = 0.64, 95% CI = 0.41-1.00; OR = 0.44, 95% CI = 0.21-0.93, respectively). The study indicated that the -2578A or -1154A allele of VEGF gene could significantly decrease the risk of adenomyosis and might be potentially protective factors for adenomyosis development.

The −2549 insertion/deletion polymorphism of VEGF gene associated with uterine leiomyoma susceptibility in women from Southeastern Iran

“…The study of Kang et al the -2578A or -1154A allele of VEGF gene significantly decreased the risk of adenomyosis and was considered potentially protective factor for adenomyosis development [30].…”

Berberine inhibits the LPS‑induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway

“…Yang et al (32) reported that abnormal secretion of IL-6 and IL-8 from endometrial stromal cells may have a role in the formation of ectopic islands in adenomyosis. Furthermore, the role of EGF, VEGF and TGF-β implicated in inflammation, angiogenesis and the pathophysiology of endometriosis has been well established (36)(37)(38). Cell invasion is a vital hallmark of tumor formation and progression.…”

Vascular endothelial growth factor gene polymorphisms contribute to the risk of endometriosis: an updated systematic review and meta-analysis of 14 case-control studies

“…According to the inclusion criteria, 14 studies were included (Hsieh et al, 2004;Bhanoori et al, 2005;Kim et al, 2005;Ikuhashi et al, 2007;Gentilini et al, 2008;Kim et al, 2008;Zhao et al, 2008;Cosin et al, 2009;Kang et al, 2009;Liu et al, 2009a,b;Attar et al, 2010;Lamp et al, 2010;Altinkaya et al, 2011). The flow chart of study selection is shown in Figure 1.…”