Vascular progenitor recruitment in critically ill patients with acute kidney injury

Chung, Yuan; Abou-Nassar, Karim; Li, Yuhua; Filion, Lionel G.; Watpool, Irene; McArdle, Tracy; McIntyre, Lauralyn; Ramsay, Timothy; Cheesman, Judy O.; Touyz, Rhian M.; Burns, Kevin D.; Allan, David

doi:10.25011/cim.v34i5.15674

Cited by 2 publications

(2 citation statements)

References 50 publications

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“…The significant advantage of ECFCs is that they can easily be isolated from adult peripheral blood, enabling auto-transplantation. Further, ECFC delivery has been characterized as suitable for application to a wide variety of tissues, including bone [ 11 , 12 ], kidney [ 13 , 14 ], and neural tissue [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

Gandhi¹,

Živković

Fisher

et al. 2015

Sensors

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Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.

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Section: Introductionmentioning

confidence: 99%

Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

Gandhi¹,

Živković

Fisher

et al. 2015

Sensors

View full text Add to dashboard Cite

show abstract

“…The discovery of circulating endothelial progenitor cells (EPCs) by Asahara et al (3,4), and further data suggesting their participation in postnatal vasculogenesis, provides evidence for vascular regeneration. EPCs multiply and differentiate into vascular endothelial cells, which facilitate vascular repair (5). Studies have revealed that EPCs differentiate into mature endothelial cells, involved in the repair of damaged endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Number and function of peripheral blood endothelial progenitor cells in Henoch-Schönlein purpura nephritis children with different degrees of renal vascular lesions

Dang

Chen

et al. 2012

Experimental and Therapeutic Medicine

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The aim of this study was to explore the correlation between different degrees of renal vascular lesions in children with Henoch-Schönlein purpura nephritis (HSPN) and changes in progenitor cell number and function in peripheral blood. Forty-eight HSPN patients were divided into three groups, mild, moderate and severe, according to the degree of renal vascular lesions. Peripheral blood mononuclear cells were isolated and cultured. Endothelial progenitor cells (EPCs) were identified by immunofluorescence assay. The number of EPCs and the migration and adhesion of EPCs were detected by flow cytometry. The numbers of peripheral blood CD34+, kinase insert domain receptor+ (KDR+) and CD133+ cells were lower in the severe and moderate vascular lesion groups compared with the mild vascular lesion group (all P<0.05) and were also lower in the severe vascular lesion group compared with the mild and moderate vascular lesion groups (all P<0.05). The adhesion and migration of EPCs were reduced in turn in the mild, moderate and severe groups. There were significant differences between the severe group and the mild and moderate groups (all P<0.05). Renal vascular lesions are involved in the occurrence and development of HSPN, while the number of EPCs, migration and adhesion of EPCs are important factors in renal vascular lesions.

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Vascular progenitor recruitment in critically ill patients with acute kidney injury

Cited by 2 publications

References 50 publications

Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

Number and function of peripheral blood endothelial progenitor cells in Henoch-Schönlein purpura nephritis children with different degrees of renal vascular lesions

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