Vascular research using human pluripotent stem cells and humoral factors [Review]

Sone, Masakatsu; Nakao, Kazuwa

doi:10.1507/endocrj.ej13-0020

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…The greatest number of cells expressed KDR (40.4%) (Figure 1 E,F). This agrees with previous reports in the mouse and human systems where KDR marked endothelial cells as well as a large population of mesodermal precursors and undifferentiated hESCs [ 15 , 16 ]. VE-CADHERIN (8.5%), CD31 (4.8%), and CD34 (13.8%) were expressed on similar-sized populations of cells and the majority of these cells showed overlap with the three markers (Figure 1 E,F).…”

Section: Resultssupporting

confidence: 93%

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

Lindgren¹,

Veldman²,

Lin³

2015

Cell Regeneration

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BackgroundEndothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the body. Ets variant 2 (ETV2) is transiently expressed in both zebrafish and mice and is necessary and sufficient for vascular endothelial cell specification. Overexpression of this gene in early zebrafish and mouse embryos results in ectopic appearance of endothelial cells. Ectopic expression of ETV2 in later development results in only a subset of cells responding to the signal.FindingsWe have examined the expression pattern of ETV2 in differentiating human embryonic stem cells (ESCs) to determine when the peak of ETV2 expression occurs. We show that overexpression of ETV2 in differentiating human ESC is able to increase the number of endothelial cells generated when administered during or after the endogenous peak of gene expression.ConclusionsAddition of exogenous ETV2 to human ESCs significantly increased the number of cells expressing angioblast genes without arterial or venous specification. This may be a viable solution to generate in vitro endothelial cells for use in research and in the clinic.

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Section: Resultssupporting

confidence: 93%

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

Lindgren¹,

Veldman²,

Lin³

2015

Cell Regeneration

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Reprogramming Approaches in Cardiovascular Regeneration

Dal‐Pra

Mirotsou

2014

Curr Treat Options Cardio Med

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Reconstitution of cardiac muscle as well as blood vessels to provide sufficient oxygenation and nutrients to the myocardium is an important component of any therapeutic approach for cardiac repair after injury. Recent reports of reprogramming somatic cells directly to cells of another lineage raised the possibility of using cell reprogramming for cardiac regenerative therapy. Here, we provide an overview of the current reprogramming strategies to generate cardiomyocytes (CMs), endothelial cells (ECs) and smooth muscle cells (SMCs), and the implications of these methods for cardiac regeneration. We also discuss the challenges and limitations that need to be addressed for the development of future therapies.

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Cell sources and methods for producing organotypic in vitro human tissue models

Hayden

2020

Organ-on-a-Chip

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Vascular research using human pluripotent stem cells and humoral factors [Review]

Cited by 3 publications

References 53 publications

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

Reprogramming Approaches in Cardiovascular Regeneration

Cell sources and methods for producing organotypic in vitro human tissue models

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