Vascular targeted chitosan-derived nanoparticles as docetaxel carriers for gastric cancer therapy

Zhang, Enhui; Xing, Ronge; Liu, Song; Li, Kecheng; Qin, Yukun; Yu, Huahua; Li, Pengcheng

doi:10.1016/j.ijbiomac.2018.12.262

Cited by 48 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CS is a non-toxic, biocompatible and biodegradable hydrophilic polymer with low immunogenicity that received FDA approval for wound dressings as well as in dietary application. In addition, the CS inherent antibacterial, antioxidant and mucoadhesive properties together with its capacity to transiently open epithelial tight junctions further enhance polymer versatility, making CS particularly suited for a variety of applications that include immunization and protein delivery [14], transmucosal and topical drug delivery [11,15], anti-cancer drug delivery [16], brain drug delivery [17] and gene delivery [18].…”

Section: Introductionmentioning

confidence: 99%

Staggered Herringbone Microfluid Device for the Manufacturing of Chitosan/TPP Nanoparticles: Systematic Optimization and Preliminary Biological Evaluation

Chiesa

Greco

Riva

et al. 2019

IJMS

View full text Add to dashboard Cite

Chitosan nanoparticles (CS NPs) showed promising results in drug, vaccine and gene delivery for the treatment of various diseases. The considerable attention towards CS was owning to its outstanding biological properties, however, the main challenge in the application of CS NPs was faced during their size-controlled synthesis. Herein, ionic gelation reaction between CS and sodium tripolyphosphate (TPP), a widely used and safe CS cross-linker for biomedical application, was exploited by a microfluidic approach based on a staggered herringbone micromixer (SHM) for the synthesis of TPP cross-linked CS NPs (CS/TPP NPs). Screening design of experiments was applied to systematically evaluate the main process and formulative factors affecting CS/TPP NPs physical properties (mean size and size distribution). Effectiveness of the SHM-assisted manufacturing process was confirmed by the preliminary evaluation of the biological performance of the optimized CS/TPP NPs that were internalized in the cytosol of human mesenchymal stem cells through clathrin-mediated mechanism. Curcumin, selected as a challenging model drug, was successfully loaded into CS/TPP NPs (EE% > 70%) and slowly released up to 48 h via the diffusion mechanism. Finally, the comparison with the conventional bulk mixing method corroborated the efficacy of the microfluidics-assisted method due to the precise control of mixing at microscales.

show abstract

Section: Introductionmentioning

confidence: 99%

Staggered Herringbone Microfluid Device for the Manufacturing of Chitosan/TPP Nanoparticles: Systematic Optimization and Preliminary Biological Evaluation

Chiesa

Greco

Riva

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Since both PLGA NPs and Ch-der NPs were internalized in HUVEC after 2 h, the difference in fluorescence intensity could depend on a different interaction with the main proteins that form the membrane of endothelial cells’ tight junctions (TJ) [48]. In fact, previous studies have shown that the mucoadhesivity of nanoparticles, e.g., those based on chitosan derivatives, improves cellular uptake [24,25,49,50]. In fact, Zambito et al [25] demonstrated that QA-Ch strongly interacted with TJ, and reduced TEER in Caco-2 cell monolayers.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of Cherry Extract Loaded in Polymeric Nanoparticles: Relevance of Particle Internalization in Endothelial Cells

et al. 2019

View full text Add to dashboard Cite

This study aimed at evaluating the anti-inflammatory effect of natural cherry extract (CE), either free or encapsulated in nanoparticles (NPs) based on chitosan derivatives (Ch-der) or poly(lactic-co-glycolic acid) (PLGA), on human umbilical vein endothelial cells (HUVEC). CE from Prunus avium L. was characterized for total polyphenols, flavonoids, and anthocyanins content. CE and CE-loaded NP cytotoxicity and protective effect on lipopolysaccharide (LPS)-stressed HUVEC were tested by water-soluble tetrazolium salt (WST-1) assay. Pro- and anti-inflammatory cytokines (TNF-α, IL-6, IL-10, and PGE2) released by HUVEC were quantified by enzyme-linked immunosorbent assay (ELISA). All NP types were internalized into HUVEC after 2 h incubation and promoted the anti-inflammatory effect of free CE at the concentration of 2 µg gallic acid equivalents (GAE)/mL. CE-loaded Ch-der NPs showed the highest in vitro uptake and anti-inflammatory activity, blunting the secretion of IL-6, TNF-α, and PGE2 cytokines. Moreover, all NPs reduced the production of nitric oxide and NLRP3 inflammasome, and had a stronger anti-inflammatory effect than the major corticosteroid dexamethasone. In particular, the results demonstrate that natural CE protects endothelial cells from inflammatory stress when encapsulated in NPs based on quaternary ammonium chitosan. The CE beneficial effects were directly related with in vitro internalization of CE-loaded NPs.

show abstract

“…A cyclic 9-mer peptide, GX1 (CGNSNPKSC), which was also screened by means of an in vivo phage display, showed a high affinity for the gastric cancer vasculature [55]. Zhang et al modified chitosan with the GE1 peptide [56]. Chitosan, whose structure is β-(1→4)-linked D-glucosamine and N-acetyl-D-glucosamine units is prepared by the deacetylation of chitin, and is used for drug delivery, regenerative medicine, wound healing and related processes [57].…”

Section: For Targeting Tumor Endothelial Cellsmentioning

confidence: 99%

Targeting Tumor Endothelial Cells with Nanoparticles

Sakurai

Akita

Harashima

2019

IJMS

View full text Add to dashboard Cite

Because angiogenesis is a major contributor to cancer progression and metastasis, it is an attractive target for cancer therapy. Although a diverse number of small compounds for anti-angiogenic therapy have been developed, severe adverse effects commonly occur, since small compounds can affect not only tumor endothelial cells (TECs), but also normal endothelial cells. This low selectivity for TECs has motivated researchers to develop alternate types of drug delivery systems (DDSs). In this review, we summarize the current state of knowledge concerning the delivery of nano DDSs to TECs. Their payloads range from small compounds to nucleic acids. Perspectives regarding new therapeutic targets are also mentioned.

show abstract

Vascular targeted chitosan-derived nanoparticles as docetaxel carriers for gastric cancer therapy

Cited by 48 publications

References 36 publications

Staggered Herringbone Microfluid Device for the Manufacturing of Chitosan/TPP Nanoparticles: Systematic Optimization and Preliminary Biological Evaluation

Staggered Herringbone Microfluid Device for the Manufacturing of Chitosan/TPP Nanoparticles: Systematic Optimization and Preliminary Biological Evaluation

Anti-Inflammatory Effect of Cherry Extract Loaded in Polymeric Nanoparticles: Relevance of Particle Internalization in Endothelial Cells

Targeting Tumor Endothelial Cells with Nanoparticles

Contact Info

Product

Resources

About