Vascularization and VEGF expression altered in bovine yolk sacs from IVF and NT technologies

Mess, Andrea; Carreira, Ana Cláudia Oliveira; Oliveira, Celso Maran de; Fratini, Paula; Favaron, Phelipe Oliveira; Barreto, Rodrigo da Silva Nunes; Pfarrer, Christiane; Meirelles, Flávio Vieira; Miglino, María Angélica

doi:10.1016/j.theriogenology.2016.09.012

Cited by 18 publications

(21 citation statements)

References 48 publications

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“…Mess et al [10] suggested a compensatory growth in response to the abnormal vascular development occurring in early pregnancy stages following an observation of overexpression of the VEGFA in IVF and in SCNT pregnancies [10]. Similar to previous results [10], we observed a significantly higher VEGFA mRNA expression in the IVF than SCNT. However, clones show substantial individual differences in differential gene expression [10].…”

Section: Discussionsupporting

confidence: 90%

“…The placental failure in bovine cloned pregnancies has been shown to be a consequence of disrupted conceptus-endometrium signalling developing during the preimplantation period [16,18]. Mess et al [10] suggested a compensatory growth in response to the abnormal vascular development occurring in early pregnancy stages following an observation of overexpression of the VEGFA in IVF and in SCNT pregnancies [10]. Similar to previous results [10], we observed a significantly higher VEGFA mRNA expression in the IVF than SCNT.…”

Section: Discussionsupporting

confidence: 89%

“…Thus, freely diffusible VEGFA-121 may possibly play a critical role during the initiation process of implantation through VEGFR-1 receptor, as speculated earlier [1]. In this study, we observed that VEGFR-1, which regulates proliferation of endothelial cells during angiogenesis [10], was significantly reduced at Day 18 in the intercaruncular endometrium of the pregnant heifers. Ferrara et al [22] indicated that a mediation function of VEGFR-1 in the vascular endothelium is to induce the release of tissue specific growth factors in a vascular bed-specific fashion.…”

Section: Discussionsupporting

confidence: 66%

“…The activities of VEGFA are mediated by the membrane receptors VEGFR-1 and VEGFR-2, which are the universal regulators of endothelial cell functions [9]. Precisely, VEGFR-1 regulates proliferation of endothelial cells during angiogenesis and VEGFR-2 is involved in migration and proliferation of endothelial cells during vessel maintenance [10].…”

Section: Introductionmentioning

confidence: 99%

“…Despite recent advances in breeding and biotechnology fields [16,17], techniques such as somatic cell nuclear transfer (SCNT) still result in low number of live births in cows. Specifically, there are severe aberrations in vascular development during the early intrauterine development that result in high embryonic losses of cloned pregnancies [10,16]. Bauersachs et al [16] as well as Mansouri et al [18] suggested that associated placentation malfunctions in clone pregnancies emanate from abnormal interactions between the conceptus and the endometrium that develop during the preimplantation phase.…”

Section: Introductionmentioning

confidence: 99%

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Vascular Endothelial Growth Factor A and VEGFR-1 Change during Preimplantation in Heifers

Chiumia

Hankele

Groebner

et al. 2020

IJMS

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Vascular endothelial growth factor A (VEGFA) plays a critical angiogenic role in the endometrium of placentalia during preimplantation. The role of VEGFA and its receptors is not fully characterised in bovine reproduction. We analysed the mRNA expression of VEGFA isoforms 121, 165 and 189, and VEGF receptors 1 and 2 in three experimental settings (A, B and C). We compared intercaruncular endometrium of cyclic to pregnant heifers at Days 12, 15 and 18 post insemination (Day 0), and between Day 15 and Day 18 conceptuses (A). We further compared caruncular versus intercaruncular endometrium at Day 15 (B), and endometrium of heifers carrying embryos originating from somatic cell nuclear transfer (SCNT) versus in vitro fertilisation (IVF) at Day 18 (C). Endometrial VEGFA protein was localised and quantified. Pregnant heifers displayed lower intercaruncular endometrial mRNA expression of VEGFA-121 (p = 0.045) and VEGFA-189 (p = 0.009) as well as lower VEGFA protein abundance (p < 0.001) at Day 15. The VEGFA protein was localised in intercaruncular luminal, glandular epithelium and in tunica muscularis of blood vessels. At Day 15, caruncular endometrium displayed higher VEGFA mRNA expression than intercaruncular endometrium (p < 0.05). Intercaruncular endometrial VEGFA protein at Day 18 was higher in abundance in SCNT than in IVF (p = 0.038). Therefore, during preimplantation in cattle, there may be a need for timely physiological reduction in intercaruncular endometrial VEGFA expression in favour of the caruncular area to facilitate a gradient towards the implantation sites. A higher expression of VEGFA in SCNT may predispose for later placentation abnormalities frequently observed following SCNT.

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