Vascularized dental pulp regeneration using cell-laden microfiber aggregates

Liang, Qingqing; Liang, Cheng; Liu, Xiaojing; Xing, Xiaotao; Ma, Shixing; Huang, He; Liang, Chao; Liu, Lei; Liao, Li; Tian, Weidong

doi:10.1039/d2tb01825j

Cited by 14 publications

(7 citation statements)

References 46 publications

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“…To verify the effect of CGF addition on pulp grafts we used SPSS 27.0 statistical analysis software, and the cross-paired chi-square test for angiogenesis between the three groups calculated that when P < 0.05 and the difference was statistically signi cant. This indicated that no new pulp-like tissue would appear inside the empty canal without the presence of pulp [33]. Some samples had murine subcutaneous bers and connective tissue growing in, which is consistent with most studies.…”

Section: Discussionsupporting

confidence: 86%

Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study

Xie¹,

Feng²,

Hu³

et al. 2023

Preprint

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Objectives This study aimed to investigate the mechanism of successful autologous pulp transplantation through semi-in situ pulp regeneration in animal experiments and three case reports of autologous pulp transplantation protocols using concentrated growth factor (CGF)-enriched pulp. Material and methods Wisdom tooth pulp was removed, placed in the anterior molar canal, and implanted in the subcranial space of the cranial apex of SD rats to establish an animal model of hemi-in situ pulp regeneration. Postoperative histological observations were performed. Three patients diagnosed with chronic periapical inflammation in a single canal of the anterior teeth and satisfied with the presence of wisdom teeth were recruited, and the CGF-rich autologous pulp transplantation protocol was selected after obtaining informed consent. Result Animal experiments showed no detachment of all SD rat grafts after surgery, a large amount of neovascularization by HE staining, and positive vascular expression by immunohistochemistry for both human CD31 and murine CD31. The three patients were followed at 3 and 6 months after surgery, and all teeth showed improvement in periapical lesions and positive pulp electrical vitality tests. Conclusion The results of animal experiments indicate that isolated pulp can survive and establish a blood supply with the host, and the addition of CGF facilitates regenerative pulp formation. The clinical results also demonstrated that CGF-rich autologous pulp transplantation protocols are a good regenerative pulp therapy (RET) for the treatment of chronic apical periodontitis in anterior teeth.

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Section: Discussionsupporting

confidence: 86%

Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study

Xie¹,

Feng²,

Hu³

et al. 2023

Preprint

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“…These observations support the limited number of other previous studies that have found DPSCs may be growth-responsive to FN administration [ 50 , 51 ]. Similarly, these results also confirm observations of DPSC responsiveness to LN administration in the few studies that have been completed to date, although these studies did not specifically use LN5 [ 52 , 53 ]. These data add to the growing evidence that ECM glycoproteins may be a significant component of the functional biomaterials needed to induce phenotypic plasticity and differentiation among DPSCs in vitro [ 54 , 55 ].…”

Section: Discussionsupporting

confidence: 83%

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Lee

Bae

Kim

et al. 2023

JFB

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) with the potential to differentiate in a limited number of other tissue types. Some evidence has suggested the modulation of DPSC growth may be mediated, in part, by exogenous extracellular matrix (ECM) glycoproteins, including fibronectin (FN) and laminin-5 (LN5). Although preliminary research suggests that some ECM glycoproteins may work as functional biomaterials to modulate DPSC growth responses, the primary goal of this project is to determine the specific effects of FN and LN5 on DPSC growth and viability. Using an existing DPSC repository, n = 16 DPSC isolates were cultured and 96-well growth assays were performed, which revealed FN, LN5 and the combination of these were sufficient to induce statistically significant changes in growth among five (n = 5) DPSC isolates. In addition, the administration of FN (either alone or in combination) was sufficient to induce the expression of alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), while LN5 induced the expression of ALP only, suggesting differential responsiveness among DPSCs. Moreover, these responses appeared to correlate with the expression of MSC biomarkers NANOG, Oct4 and Sox2. These results add to the growing body of evidence suggesting that functional biomaterials, such as ECM glycoproteins FN and LN5, are sufficient to induce phenotypic and differentiation-specific effects in a specific subset of DPSC isolates. More research will be needed to determine which biomarkers or additional factors are necessary and sufficient to induce the differentiation and development of DPSCs ex vivo and in vitro for biomedical applications.

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“…A nude mouse model was established following the methodology outlined in previous research [21]. Forty healthy premolars were extracted from patients undergoing orthodontic therapy, with informed consent obtained.…”

Section: Ectopic Pulp Regeneration In a Nude Mouse Modelmentioning

confidence: 99%

Chemically Modified MicroRNA Delivery via DNA Tetrahedral Frameworks for Dental Pulp Regeneration

Wei,

Xu,

Zhou

et al. 2024

Preprint

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Dental pulp regeneration is a promising strategy for addressing tooth disorders. Incorporating this strategy involves the fundamental challenge of establishing functional vascular networks using dental pulp stem cells (DPSCs) to support tissue regeneration. Current therapeutic approaches lack efficient and stable methods for activating DPSCs. In the study, we used a chemically modified microRNA (miRNA)-loaded tetrahedral-framework nucleic acid nanostructure to promote DPSC-mediated angiogenesis and dental pulp regeneration. Incorporating chemically modified miR-126-3p into tetrahedral DNA nanostructures (miR@TDNs) represents a notable advancement in the stability and efficacy of miRNA delivery into DPSCs. These nanostructures enhanced DPSC proliferation , migration, and upregulated angiogenesis-related genes, enhancing their paracrine signaling effects on endothelial cells. This enhanced effect was substantiated by improvements in endothelial cell tube formation, migration, and gene expression. Moreover, in vivo investigations employing matrigel plug assays and 1 ectopic dental pulp transplantation confirmed the potential of miR@TDNs in promoting angiogenesis and facilitating dental pulp regeneration. Our findings demonstrated the potential of chemically modified miRNA-loaded nucleic acid nanostructures in enhancing DPSC-mediated angiogenesis and supporting dental pulp regeneration. These results highlighted the promising role of chemically modified nucleic acid-based delivery systems as therapeutic agents in regenerative dentistry and tissue engineering.

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Vascularized dental pulp regeneration using cell-laden microfiber aggregates

Abstract: Regeneration of dental pulp by transplantation of dental pulp stem cells (DPSCs) emerges as a novel therapy for dental pulp necrosis after inflammation and injury. However, how to provide sufficient...

Cited by 14 publications

References 46 publications

Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study

Pulp regeneration by transplantation of dental pulp with the synergy of concentrate growth factor: An in vitro and in vivo study

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Chemically Modified MicroRNA Delivery via DNA Tetrahedral Frameworks for Dental Pulp Regeneration

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