Vasoactive intestinal peptide in the hamster seminal vesicle: Distribution, binding sites and possible functions

Pinho, Mário; Sebastião, Ana M.; Rodrigues, Gabriela; Barroso, C; Ribeiro, J.A.; Mata, Lucinda R.; Gulbenkian, S.

doi:10.1016/0306-4522(94)90307-7

Cited by 11 publications

(15 citation statements)

References 36 publications

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“…This finding accords with the results of previous studies describing the distribution of nerve fibres containing acetylcholinesterase (AChE) or VAChT in the human SV (Gosling and Dixon, 1994;Sjöstrand et al, 1998;Dixon et al, 2000) as well as in the SV and BU of guinea pigs (Al-Zuchair et al, 1975;Sjöstrand et al, 1998), rats (Moss et al, 1987;Yuri, 1990) and hamsters (Pinho et al, 1994;Chow et al, 1997).…”

Section: Discussionsupporting

confidence: 92%

“…The ovine prostate contained VIP-IR nerve fibres in a frequency comparable to the one found in the SV and BU but the abundance of NPY-IR nerve fibres was very low. Previously, numerous VIPand NPY-containing nerve fibres were found in the human ASG (Gu et al, 1983;Adrian et al, 1984;Higgins and Gosling, 1989;Lange and Unger, 1990;Chapple et al, 1991;Tainio, 1995;Hedlund et al, 1997;Dixon et al, 2000) and in the ASG of many other animal species (Alm et al, 1977(Alm et al, , 1980Stjernquist et al, 1983;Lamano-Carvalho et al, 1986;Moss et al, 1987;Yuri, 1990;Properzi et al, 1992;Iravani and Zar, 1994;Pinho et al, 1994;Ottesen et al, 1995;Domeneghini, 1997, 1998;Chow et al, 1997;Kaleczyc et al, 1999;Czaja et al, 2000;Majewski et al, 2001;Mirabella et al, 2003). The results of the previously mentioned studies can be summarised as follows: in all species the vast majority of VIP-IR nerve fibres were localised in the mucosal layer of the SV, BU and prostate, whereas only sparse VIP-IR nerve fibres were found within the smooth muscle layer.…”

Section: Discussionmentioning

confidence: 97%

“…The latter was also found to contract smooth muscles of the guinea-pig prostate (Cohen and Drey, 1989). Numerous reports have indicated that VIP and NPY can directly influence the activity of the male reproductive system (Larsen et al, 1981;Stjernquist et al, 1983;Smith et al, 1984;Fahrenkrug et al, 1988;Iravani and Zar, 1994;Pinho et al, 1994;Tong et al, 1996) probably also due to their interactions (Solano et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

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Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

Arciszewski¹,

Wąsowicz

2006

Acta Veterinaria Hungarica

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Immunohistochemistry was applied to determine the distribution patterns of nerve fibres containing tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and vesicular acetylcholine transporter (VAChT) in the prostate, seminal vesicle (SV) and bulbourethral glands (BU) of male sheep. In all organs studied, cholinergic innervation was more developed than noradrenergic innervation. Numerous VAChTimmunoreactive (IR) nerve fibres were found in the muscular layer and mucosa of the SV and BU as well as in the prostate. A similar abundance of noradrenergic nerve fibres (showing immunoreactivity both to TH and DβH) was also found in both layers of the SV and BU (but not in the prostate). In the prostate a moderate density of VIP-IR nerve fibres was present but only very scarce NPY-IR nerve fibres were shown. All the studied accessory sexual glands (ASG) of male sheep contained VIP-IR nerve fibres in a similar frequency. Double immunohistochemistry revealed that the vast majority of noradrenergic nerve fibres also contained NPY. None of the noradrenergic nerve fibres showed the presence of VAChT or VIP. The possible functional significance of these findings is discussed.Key words: Autonomic innervation, neuropeptides, immunohistochemistry, accessory sexual glands, genital tract, sheep Secretions of the accessory sexual glands (ASG) contribute to the semen plasma responsible for the maintenance of a favourable environment for spermatozoa. It is generally accepted that dysfunctions of the prostate, seminal vesicles (SV) and bulbourethral glands (BU) lead to low semen quality, making the semen unsuitable for artificial insemination (AI). This is of particular importance in ruminants in which AI is routinely used. The general opinion is that the development and function of the ASG are influenced both by the endocrine and the nervous system. The application of histochemical and immunohistochemical techniques has revealed autonomic nerve fibres (noradrenergic and cholinergic)

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

Arciszewski¹,

Wąsowicz

2006

Acta Veterinaria Hungarica

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“…Very little is known about the physiological role of VIP in AGG. Pinho et al (1994) reported that in the seminal vesicle of the hamster VIP-IR nerve fibres also manifested AChE activity and suggested a modulatory function of this peptide on muscarine receptors. VIP is typically present in cholinergic nerves in other tissues (Ottesen and Fahrenkrug, 1995).…”

Section: Discussionmentioning

confidence: 97%

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Klimczuk¹,

Kaleczyc²,

Franke-Radowiecka³

et al. 2005

Vet. Med. - Czech

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Our previous immunohistochemical investigations revealed three major populations of nerve fibres supplying the porcine accessory genital glands (AGG) including noradrenergic, non-noradrenergic putative cholinergic and sensory nerve terminals (Kaleczyc et al., 1997). However, it is still unclear whether the non-noradrenergic nerve fibres are cholinergic in nature. The knowledge of the population of cholinergic nerve fibres in mammalian AGG based upon vesicular acetylcholine transporter (VAChT) immunohistochemistry is very limited. Therefore, the aim of the present investigation was to disclose the occurrence and colocalization patterns of VAChT, dopamineâ-hydroxylase (DβH) and some neuropeptides including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and somatostatin (SOM) within nerve fibres supplying the porcine AGG. Double-immunohistochemical labellings showed that VAChT-positive nerve terminals were non-adrenergic (DâH-negative), however, many of them contained immunoreactivities to VIP, NPY and/or SOM. The coexistence patterns of these biologically active substances in nerve fibres supplying particular glands are similar but the density of cholinergic innervation varies between the organs. The innervation of the seminal vesicle and prostatic body is better developed than that of the disseminated part of the prostate and bulbourethral glands. The majority of cholinergic nerve fibres associated with blood vessels supplying the glands contain VIP and NPY and, to a lesser degree, SOM. The possible function and origin of the cholinergic nerve fibre population are discussed.

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“…neurotransmitters and neuropeptides. Indeed, it has subsequently been demonstrated that vasoactive intestinal peptide (VIP) interferes with the secretory activity of the hamster seminal vesicle (Pinho et al 1994) and that this gland displays, in its muscular coat and blood vessels, VIP-binding sites whose number is affected by testosterone (Pinho et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture

Rodrigues

Cachaço

Gulbenkian

et al. 1999

Cell and Tissue Research

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In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens.

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Vasoactive intestinal peptide in the hamster seminal vesicle: Distribution, binding sites and possible functions

Cited by 11 publications

References 36 publications

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Vasoactive intestinal peptide stimulates apical secretion in hamster seminal vesicle epithelial cells in culture

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