Vasoactive intestinal peptide stimulates ciliary motility in rabbit tracheal epithelium: modulation by neutral endopeptidase

Sakai, Naotaka; Tamaoki, Jun; Kobayashi, Kenji; Kanemura, Toshinori; Isono, Kazuo; Takamatsu, Kiyoshi; Takeuchi, Satomi; Takizawa, Takao

doi:10.1016/0167-0115(91)90222-3

Cited by 19 publications

(15 citation statements)

References 28 publications

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“…Constitutive production of mucins by GCs maintains the mucus layer for clearance of inhaled particles through ciliary movement which is promoted by VIP [31]. Goblet cell metaplasia can be induced by T H 2 cytokines [27], and the VIP/VPAC 2 axis promotes a T H 2 response as reported in the VPAC 2 transgenics [37].…”

Section: Discussionmentioning

confidence: 99%

The absence of the VPAC2 receptor does not protect mice from Aspergillus induced allergic asthma

2010

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Allergic asthma is a T H 2-mediated disease marked by airway inflammation, increased mucus production, and elevated serum IgE in response to allergen provocation. Among its ascribed functions, the neuropeptide Vasoactive Intestinal Peptide (VIP) is believed to promote a T H 2 phenotype when signaling through its VPAC 2 receptor. In this study, we assessed the requirement for the VIP/VPAC 2 axis in initiating the allergic pulmonary phenotype in a murine model of fungal allergic asthma. C57BL/6 wild-type (WT) and VPAC 2 knock-out (KO) mice were sensitized with Aspergillus fumigatus antigen and challenged with an aerosol of live conidia to induce allergic airways disease. WT and KO mice exhibited similar peribronchovascular inflammation, increased number of goblet cells, and elevated serum IgE. However, the absence of VPAC 2 receptor resulted in a marked enhancement of MUC5AC mRNA with an associated increase in goblet cells and a reduction in eosinophils in the airway lumen at day 3 when VIP mRNA was undetectable in the KO lung. Sustained elevation of serum IgE was noted in KO mice at day 14, while the level in WT mice declined at this time point. These data suggest that the absence of VPAC 2 does not protect mice from developing the signs and symptoms of allergic asthma.

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Section: Discussionmentioning

confidence: 99%

The absence of the VPAC2 receptor does not protect mice from Aspergillus induced allergic asthma

2010

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“…The ciliated columnar cells also perform a vital role in clearance of inhaled material via the mucociliary escalator. VIP predominantly localized to the columnar epithelia of the airways in naïve lungs which may further establish its roles in enhancing ciliary beat [22] and wound healing [23]. Its availability in the columnar epithelium may enable its secretagogue effect on mucus secreting cells [24, 25] that intersperse this cell layer thereby leading to an increase in mucus production.…”

Section: Discussionmentioning

confidence: 99%

Spatio-temporal localization of vasoactive intestinal peptide and neutral endopeptidase in allergic murine lungs

Samarasinghe

Hoselton

Schuh

2010

Regulatory Peptides

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Vasoactive intestinal peptide (VIP) is a neuropeptide with cytokine properties that is abundant in the lung. VIP null mice exhibit spontaneous airway inflammation and hyperresponsiveness emphasizing VIP's "anti-asthma" potential. Although VIP's impending protective role in the lung has been demonstrated, its localization in the naïve and allergic murine lungs has not. To this aim, we analyzed the availability of VIP and its protease, neutral peptidase (NEP), in naïve and Aspergillus-sensitized and challenged murine lungs after 3, 7, and 14 days. Both VIP and NEP were predominantly localized to the columnar epithelia of the airways in naïve lungs. A marked decrease in VIP occurred in these cells 3 days after allergen challenge. NEP localization in the columnar epithelia decreased after allergen challenge. At day 14, VIP localization in the columnar epithelia and arteriolar smooth muscle increased while NEP localization at these sites remained low. This study provides new insights into the local regulation of VIP in the columnar epithelia of the allergic lung. Its altered availability in the context of allergy provides fresh evidence for the modulation of pulmonary inflammation by VIP.

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“…In a clinical setting, an increase of VIP concentration was observed as a result of NEP inhibition in hypertensive patients. (Sakai et al, 1991;Duggan et al, 1994;Ye et al, 2004;M arton et al, 2005;Rahardjo et al, 2013). These discrepancies might be due to the fact that our study used human tissue not affected by a pathological condition (the CCP was obtained from healthy men with a mean age of 32 years).…”

Section: Discussionmentioning

confidence: 94%

Effects of endopeptidase inhibition on the relaxation response of isolated human penile erectile tissue to vasoactive peptides

Rahardjo

Reichelt

Sonnenberg³

et al. 2016

Andrologia

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Peptides, such as CNP, CGRP and VIP, are involved in the function of male penile erectile tissue. Tissue levels of said peptides are controlled by the endopeptidase enzymes. Theoretically, the inhibition of the degradation of CNP, CGRP and/or VIP should result in an enhancement in penile smooth muscle relaxation. The effects were investigated of CNP or VIP (0.1 nm-1 μm), without and following pre-exposure of the tissue to a threshold concentration of the endopeptidase inhibitor KC 12615 (10 μm, for 20 min), on the reversion of tension induced by means of electrical field stimulation. Drug effects on the production of cyclic AMP/GMP were also evaluated. Neither KC 12615, CNP and VIP nor the combination of CNP plus KC 12615 or VIP plus KC 12615 increased the response of the tissue to EFS. While no effects were observed of a pre-exposure of the tissue to KC 12615 on the production of cyclic AMP in the presence of VIP, an enhancement was registered in the accumulation of cyclic AMP in the presence of CNP plus KC 12615. Further studies are indicated to investigate whether endopeptidase inhibitors might tend to be more effective in tissues affected by a decreased local production of vasoactive peptides.

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Vasoactive intestinal peptide stimulates ciliary motility in rabbit tracheal epithelium: modulation by neutral endopeptidase

Cited by 19 publications

References 28 publications

The absence of the VPAC2 receptor does not protect mice from Aspergillus induced allergic asthma

The absence of the VPAC2 receptor does not protect mice from Aspergillus induced allergic asthma

Spatio-temporal localization of vasoactive intestinal peptide and neutral endopeptidase in allergic murine lungs

Effects of endopeptidase inhibition on the relaxation response of isolated human penile erectile tissue to vasoactive peptides

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