Vasodepressor Effects of Exercise are Accompanied by Reduced Circulating Ouabainlike Immunoreactivity and Normalization of Nitric Oxide Synthesis

Abstract

Our object was to evaluate the effects of regular mild exercise on blood pressure and on circulating level of ouabainlike factors (OLF) and of nitrate anion, an endproduct of nitric oxide (NO) in humans. We measured plasma ouabainlike immunoreactivity (OLI) and nitrate ions (NO3.) before and after mild exercise for 3 months' duration in 16 patients with essential hypertension, diabetes mellitus, obesity, or hyperlipidemia. Plasma OLI was measured using an amplified ELISA system with anti-ouabain antibody and biotinyl-tyramide. Serum NO3. was measured with high-performance liquid chromatography (HPLC) with an anion-exchange column. With the reverse phase HPLC system with an octa decylsilyl silicagel column, the elution volume of plasma OLI of a healthy volunteer matched that of authentic ouabain in a gradient elution system of acetonitrile/H2O. Plasma OLI levels decreased significantly by about 34% after mild exercise, and NO3. levels tended to be within the reference interval in normal volunteers. Body weight, diastolic and systolic blood pressure, serum triglyceride and acetylcholine esterase (a marker of the fatty liver) were significantly decreased (p < 0.01) after 3 months of regular mild exercise. The plasma OLI level was significantly correlated with plasma NO3., there was a trend toward a correlation with diastolic blood pressure (p = 0.06) before and after regular exercise. Regular mild exercise led to a decrease in plasma levels of OLI, and acetylcholine esterase activity and blood pressure in adult patients. Results suggest that changes in OLF production contribute to the blood pressure regulation seen in patients who exercise regularly.

Increases in Plasma Ouabainlike Immunoreactivity during Surgical Extirpation of Pheochromocytoma.

“…the operation. Before operation, plasma OLI levels in this patient were within the reference interval of normal volunteers (41.4 ± 13.4 pg/ml) (10). Plasma levels of OLI increased during the operation, and peaked when the tumor was mechanically pressed for excision.…”

A novel endogenous digitalis, telocinobufagin, exhibits elevated plasma levels in patients with terminal renal failure

“…However, the plasma levels of ouabain-like immunoreactivity (0.019 nmol/L = 12 pg/mL) in their study were almost equal to the findings (9 pg/ mL) in our previous enzyme-linked immunosorbent assay (ELISA) [13,20] and LC/MS analysis [12]. Binding proteins for EDLFs have been reported by Schoner's group and by us, and the activity of these proteins may of course affect the results of the assay [21,22].…”

“…The minimum detection limit was defined as the sample concentration resulting in a peak area of three times the noise level, and it was 1 ng/mL. When 1 to 100 ng/mL of MBG and TCB were used as a quality control sample Table 3 The chemical shift values of TCB in 13 The vacant columns represent the places where no chemical shift values could be detected. The structure and carbon numbers correspond to those shown in Fig.…”

“…For patients who were treated with regular hemodialysis in our hospital, samples were withdrawn just before the hemodialysis. Ten milliliters of venous blood was taken from the antecubital vein into an EDTA-2K containing (1 mg/mL) tube as previously described [13]. After centrifugation at 3500 rpm for 15 min, the plasma was stored in a deep freezer (À808C) until use in 1 mL aliquots.…”

Ouabain-Like Compound Changes Rapidly on Physical Exercise in Humans and Dogs

“…Previous studies showed ambiguous results. An increase 9 as well as a decrease of plasma concentrations of ouabain-like compounds (OLCs) 10,11 have been reported. Here, we investigated the effect of physical exercise on the endogenous ouabain plasma concentration in several experimental settings as well as the influence of ␤-blockade and angiotensin-converting enzyme (ACE) inhibition.…”