Vaspin gene expression in human adipose tissue: Association with obesity and type 2 diabetes

Klöting, Nora; Berndt, Janin; Kralisch, Susan; Kovacs, Peter; Faßhauer, Mathias; Schön, Michael P.; Stümvoll, Michael; Blüher, Matthias

doi:10.1016/j.bbrc.2005.11.039

Cited by 307 publications

(274 citation statements)

References 16 publications

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“…In these rats, serum vaspin levels decreased with worsening of diabetes but could be normalised by insulin or pioglitazone treatment. Consistent with these observations, we found that higher serum vaspin concentrations and increased SERPINA12 mRNA expression in human adipose tissue was associated with obesity, insulin resistance and type 2 diabetes [2,3]. In the Kooperative Gesundheitsforschung in der Region Augsburg [Cooperative Health Research in the Augsburg Region] (KORA) study, vaspin genotype was found to be significantly associated with type 2 diabetes independently of obesity [4].…”

Section: Introductionsupporting

confidence: 81%

“…Human studies Human SERPINA12 mRNA expression was measured as described previously [2] in the stomach explants of five morbidly obese, but otherwise healthy, insulin-sensitive individuals who underwent gastric sleeve resection [6]. Immunostaining of paraffin sections from these stomach samples was performed using an anti-vaspin monoclonal antibody (AdipoGen) as described previously [3].…”

Section: Methodsmentioning

confidence: 99%

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Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects

Kovacs²,

et al. 2011

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Aims/hypothesis Vaspin (visceral adipose tissue-derived serpin) was first identified as an adipokine in a rat model of type 2 diabetes, in which it is predominantly secreted from visceral adipose tissue. Serum concentrations of vaspin show a food intake-related diurnal variation. We therefore tested the hypothesis that vaspin plays a role in the regulation of food intake. Methods Vaspin levels in the hypothalamus and human stomach were determined by western blotting. The cerebrospinal fluid concentration of vaspin was measured in five healthy volunteers using an ELISA. Fed 11-week-old female db/db mice were given intraperitoneal injections of 1 mg/kg body weight of vaspin (n=6) or saline (n=6) on experimental days 1, 3 and 4. Changes in food intake and fed plasma glucose concentrations were determined after one intracerebroventricular administration of either 1 μg vaspin or artificial cerebrospinal fluid into 11-week-old female db/db (n=8) and C57BL/6 mice (n=8) up to 6 days after injection. Results We detected vaspin in the hypothalamus of db/db and C57BL/6 mice and in the cerebrospinal fluid of healthy individuals. Both peripheral and central vaspin administration decrease food intake in obese db/db and lean C57BL/6 mice. In db/db mice, vaspin treatment is associated with sustained glucose-lowering effects for at least 6 days after injection. In addition, we demonstrated expression of the gene encoding vaspin in the gastric mucosa in humans, and found that this was subject to regional variations. Conclusions/interpretation Our data suggest a previously unrecognised role of vaspin in the regulation of food intake. We postulate that vaspin inhibits a protease that degrades an anti-orexigenic factor.

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Section: Introductionsupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects

Kovacs²,

et al. 2011

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“…Most studies found higher vaspin levels in adult women compared with men, 6,8,[16][17][18] whereas others did not. 7,9,19 According to our data, vaspin increased with puberty in girls, but not in boys. Interestingly, vaspin was also found to be increased in patients with polycystic ovary syndrome 10 and treatment with estrogen/progesterone 20 or oral contraceptives 17 increased vaspin in polycystic ovary syndrome patients and healthy obese women.…”

Section: Discussionmentioning

confidence: 42%

“…9,10,21,22 Hence, other sources may express vaspin and Vaspin and glucose metabolism in children A Körner et al contribute to circulating levels and their regulation. Indeed, Fain et al 22 found vaspin mRNA enriched in non-fat cells of the stromal-vascular fraction.…”

Section: Discussionmentioning

confidence: 99%

“…3 In humans, some subsequent studies confirmed the association of vaspin with obesity and BMI, 4,5 although the association was more subtle 6 or absent 7,8 in other studies. Several studies found a positive correlation of circulating vaspin with insulin resistance indices 5,9,10 but not with the presence of overt type 2 diabetes. [6][7][8] A recent study described a marked diurnal and meal-associated pattern of circulating vaspin levels, which was reciprocal to that of insulin and glucose, and closely associated with meal ingestion.…”

Section: Introductionmentioning

confidence: 99%

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Vaspin is related to gender, puberty and deteriorating insulin sensitivity in children

Körner¹,

Neef²,

Friebe³

et al. 2010

Int J Obes

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Background: Visceral adipose tissue-derived serine protease inhibitor (vaspin) has been suggested as a novel adipocytokine related to obesity and insulin sensitivity in adults. Design: We quantified vaspin serum concentrations in 65 lean and 67 obese children and aimed to evaluate the relationship of vaspin with physical development, obesity, and metabolic and cardiovascular phenotypes in children. We further assessed the acute vaspin response to glucose provocation in 20 obese adolescents and evaluated tissue expression patterns of vaspin in humans. Results: Vaspin levels were significantly higher in girls than in boys. In girls, vaspin increased with age and pubertal stage, whereas there was no change with development in boys. Obese girls had lower vaspin serum levels than those of lean controls, but there was no significant correlation with body mass index (BMI). Independent of sex, age and BMI, lower vaspin was associated with better insulin sensitivity, with higher systolic blood pressure and impaired endothelial function. In response to glucose provocation during an oral glucose tolerance test, vaspin serum levels declined by approximately 25% in adolescents with hyperinsulinemia, whereas there was no significant decline in normoinsulinemic patients. In support of our clinical data, we not only confirmed vaspin mRNA expression in adipose tissue but also found consistent expression of vaspin in the liver and indications for expression in the pancreas and the skin. Conclusion: We showed that gender differences in circulating vaspin levels develop during pubertal progression in girls. Although vaspin's association with obesity remains controversial, vaspin was increased with worsening insulin resistance already in children and was acutely down-regulated following glucose provocation in insulin-resistant adolescents independent of obesity. Besides adipose tissue, vaspin expression in the liver and the pancreas may potentially contribute to circulating vaspin levels and their regulation.

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Adipose Tissue and Overweight

Levine¹,

Levine²

2012

Metabolic Syndrome and Cardiovascular Disease

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Vaspin gene expression in human adipose tissue: Association with obesity and type 2 diabetes

Cited by 307 publications

References 16 publications

Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects

Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects

Vaspin is related to gender, puberty and deteriorating insulin sensitivity in children

Adipose Tissue and Overweight

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