Vegetative characterization to identify oil palm (Elaeis guineensis Jacq.) plantlet abnormalities

Ernayunita, Ernayunita; Rahmadi, Hernawan Yuli; Yenni, Yurna; Setiowati, Retno Diah; Harahap, Iman Yani

doi:10.1063/1.5098409

Cited by 3 publications

(6 citation statements)

References 7 publications

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“…Another identification is in the vegetative stage of plantlets to seedlings, which causes vegetative abnormalities in the clones. [30] grouped abnormal clones vegetatively into plantlet, pre-nursery, and main nursery phases. In the plantlet phase, plantlet abnormalities were rosette, dry tips, erect, pseudo plantlets, less than four leaves, white tips, dwarf, curved, terminal inflorescence, and yellowish.…”

Section: Identifying Processes In the Laboratory That Can Cause Clone...mentioning

confidence: 99%

“…In addition, the research continued to field testing to determine the effect of callus formation time on clone abnormalities in the field [25]. Vegetative grouping of normal and abnormal clones was carried out in the plantlet, pre-nursery and main nursery phases [30], and field testing was carried out to determine the performance of clones in the field from an early age with routine monitoring and evaluation [8]. In addition, research for efficiency in clone production in the laboratory was also carried out, including agar substitution [37], optimizing the number of plantletsand adding NAA in plantlet growth [38], and research related to lightintensity and filters on development of oil palm culture [39].…”

Section: Various Research and Innovationmentioning

confidence: 99%

“…In addition, research for efficiency in clone production in the laboratory was also carried out, including agar substitution [37], optimizing the number of plantletsand adding NAA in plantlet growth [38], and research related to lightintensity and filters on development of oil palm culture [39]. Other research for plantlet distribution was by optimization of acclimatization research [40], sending of plantlets [41] and clone seeds [42], use of natural light during the hardening period [43] immersion of plantlets in water as pre-aclimatization of plantlets [44], optimization of sterilization protocols to reduce culture contamination [45], and standardization of seedling quality [46].…”

Section: Various Research and Innovationmentioning

confidence: 99%

“…During its development period, oil palm tissue culture faced somaclonal variations in the form of abnormal floral of oil palm clones. This problem was faced by various laboratories that develop tissue cultures [4,6,7]. Abnormalities in oil palmclones can indeed have a negative impact on oil palm productivity.…”

Section: Introductionmentioning

confidence: 99%

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Towards the fourth decade of IOPRI’s oil palm clones: Upcoming new variety

Ernayunita,

Yenni,

Rahmadi

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Experience of IOPRI on oil palm tissue culture would be four decades in 2024. The first decade was theperiod of oil palm clone development and field testing. The second decade was a setback for oil palm research because of a high percentage of oil palm clone abnormalities in the field. Moving on from these experiences, tissueculture systems such as: including improving the protocol, controlling the duration of callus induction, delimitating the number of subcultures, and building of oil palm clones database, were managed to be improved. In this way, alow abnormality level is under control and each clone is traceable. It is also noted that the oil palm clone provides20-30% higher production than that derived from DxP crossing seed due to the more uniform growth of generatedplants. Besides the protocol, ortet selection is also key to improvement. Ortets are elite plants from selected progeny testing or re-clone of the progeny testing. Molecular analysis is applied as a control of genetic variation possibility, which may occur in a particular phase due to specific stimuli. However, until the present time, a clonevariety has not been released yet. Therefore, in the fourth decade, mass production is expected and oil palm clonevarieties will officially be released and commercialized

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Section: Identifying Processes In the Laboratory That Can Cause Clone...mentioning

confidence: 99%

Section: Various Research and Innovationmentioning

confidence: 99%

Section: Various Research and Innovationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Towards the fourth decade of IOPRI’s oil palm clones: Upcoming new variety

Ernayunita,

Yenni,

Rahmadi

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

Self Cite

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“…Abnormalitas pembungaan berupa buah mantel tidak terdeteksi pada kultur embrioid fase pertama dan mulai terdeteksi adanya abnormalitas setelah dilakukan multiplikasi berulang kali, yakni tingkat abnormalitas sebesar 25% ditemukan di tahun kedua dan menjadi 90% di tahun ketiga penanaman di lapangan. Jenis abnormalitas lainnya yang ditemukan pada klon kelapa sawit adalah buah partenokarpi, kemandulan buah, perpanjangan masa juvenil, bunga abortus, serta pertumbuhan abnormal vegetatif (Soh et al, 2011;Guzman and Peralta 2010;Ernayunita et al, 2019).…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh Waktu Paparan Zat Pengatur Tumbuh Terhadap Tingkat Abnormalitas Klon Kelapa Sawit

Pratiwi¹,

Wening²,

Nazri³

2020

J. Penelitian Kelapa Sawit

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Salah satu komponen yang sering terdapat dalam media kultur jaringan tanaman adalah Zat Pengatur Tumbuh (ZPT), yang diperlukan untuk merangsang pertumbuhan kultur tanaman. Pemberian ZPT yang berlebihan dapat memberikan dampak abnormalitas pada tanaman klon hasil kultur jaringan. Diperlukan kajian untuk mengetahui pengaruh waktu paparan ZPT terhadap tingkat abnormalitas klon secara komprehensif. Penelitian ini bertujuan untuk mengetahui pengaruh total waktu paparan ZPT serta waktu paparan beberapa media dengan kandungan ZPT yang berbeda terhadap tingkat abnormalitas tanaman klon kelapa sawit di lapangan. Empat galur tanaman hasil kultur jaringan (klon) kelapa sawit yakni P, Q, R, dan S yang berumur 4 tahun digunakan untuk penelusuran waktu inkubasi kultur di laboratorium dan pengamatan tingkat abnormalitas di lapangan. Hubungan antara peubah dianalisis menggunakan analisis korelasi dan analisis regresi linier berganda serta analisis regresi linier sederhana. Hasil menunjukkan bahwa total waktu paparan kultur pada media yang mengandung ZPT berpengaruh secara nyata pada tingkat abnormalitas klon di lapangan. Semakin lama waktu paparan (lebih dari 171 hari), semakin tinggi tingkat abnormalitas klon. Dari ketiga jenis media kultur yang mengandung ZPT, media yang mengandung 2,4-D dan 2,4,5-TCPP memiliki pengaruh yang signifikan dan hubungan positif yang sangat erat terhadap kejadian abnormalitas klon dibandingkan media yang mengandung 2,4-D dan BAP. Lama waktu paparan dengan NAA tidak berpengaruh nyata terhadap tingkat abnormalitas klon di lapangan. Sebaliknya, total waktu inkubasi media kultur yang tidak mengandung ZPT tidak mempengaruhi tingkat abnormalitas klon di lapangan dan hubungannya negatif. Model linier dapat digunakan untuk memperkirakan tingkat abnormalitas klon yang dihasilkan berdasarkan waktu paparan ZPT pada proses in vitro.

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Optimasi sistem kultur dan media untuk peningkatan tinggi tunas in vitro kelapa sawit

Sinta

Saptari

Riyadi

et al. 2023

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Optimization of in vitro shoot growth is necessary to shorten the culture time and produce vigorous oil palm plantlets. This research was conducted to determine the best media and culture techniques to accelerate in vitro shoot growth of oil palm. Shoots of oil palm derived from somatic embryogenesis were grown on DF media with two culture systems (solid medium and double-layer) combined with hormone treatments (0.5-1 mg L-1 giberellin/GA3 and 0.5 mg L-1 Benzyl amino purine/BAP or thidiazuron/ TDZ). Further optimization was conducted using different bottle closures (polypropylene screw caps and plastic wraps) and macronutrients (standard or double concentrations). This research was conducted using a completely randomized design (CRD), with each treatment consisting of 5 bottle replications and each bottle consisting of 5 shoots. The results showed that media with double-layer system combined with GA3 (0.5-1 mg L-1) and TDZ (0.5 mg L-1) gave the highest shoot height increment. The use of double-strength macronutrient media combined with screw bottle caps increased shoot height (136-167 %) and decreased shoot tip necrosis (0-24 %). Plastic wrap bottle caps increased shoot tip necrosis (STN), while doubling macronutrients reduced STN. The growth of oil palm shoots began to slow down after 5 weeks of culture. In conclusion, the optimal conditions for in vitro shoot growth of oil palm were at usage of double-layer media added with GA3 0.5-1 mg L-1, TDZ 0.5 mg L-1, and double macronutrients on bottle jars with polypropylene screw caps.

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Vegetative characterization to identify oil palm (Elaeis guineensis Jacq.) plantlet abnormalities

Cited by 3 publications

References 7 publications

Towards the fourth decade of IOPRI’s oil palm clones: Upcoming new variety

Towards the fourth decade of IOPRI’s oil palm clones: Upcoming new variety

Pengaruh Waktu Paparan Zat Pengatur Tumbuh Terhadap Tingkat Abnormalitas Klon Kelapa Sawit

Optimasi sistem kultur dan media untuk peningkatan tinggi tunas in vitro kelapa sawit

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