Venenum bufonis: An overview of its traditional use, natural product chemistry, pharmacology, pharmacokinetics and toxicology

Wei, Wenlong; Hou, Jinjun; Wang, Xia; Yu, Yang; Li, Hao-Jv; Li, Zhenwei; Feng, Zijin; Qu, Hua; Wu, Wanying; Guo, De‐An

doi:10.1016/j.jep.2019.03.042

Cited by 44 publications

(32 citation statements)

References 99 publications

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“…A recent study showed that the venoms of Rhinella schneideri and R. marina exhibit a strong tumor growth inhibition toward drug-sensitive CCFR-CEM and P-glycoprotein overexpressing CEM/ADR5000 leukemia cells. Moreover, Venenum Bufonis (VB), a product of the secretions of Bufo gargarizans Cantor and B. melanostictus Schneider, which has been long used as Traditional Chinese Medicine exhibit antitumor, antinociceptive and anti-inflammatory activities in different types of cancer [ 83 ]. It appears that these effects are mainly due to bufadienolides [ 82 , 83 ].…”

Section: Zootherapymentioning

confidence: 99%

Zoopharmacology: A Way to Discover New Cancer Treatments

et al. 2020

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Zoopharmacognosy is the multidisciplinary approach of the self-medication behavior of many kinds of animals. Recent studies showed the presence of antitumoral secondary metabolites in some of the plants employed by animals and their use for the same therapeutic purposes in humans. Other related and sometimes confused term is Zootherapy, which consists on the employment of animal parts and/or their by-products such as toxins, venoms, etc., to treat different human ailments. Therefore, the aim of this work is to provide a brief insight for the use of Zoopharmacology (comprising Zoopharmacognosy and Zootherapy) as new paths to discover drugs studying animal behavior and/or using compounds derived from animals. This work is focused on the approaches related to cancer, in order to propose a new promising line of research to overcome multidrug resistance (MDR). This novel subject will encourage the use of new alternative prospective ways to find new medicines.

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Section: Zootherapymentioning

confidence: 99%

Zoopharmacology: A Way to Discover New Cancer Treatments

et al. 2020

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“…Detected and calculated [M+H] + ions were compared and the error was calculated for the tentative identification of the different compounds. Data analyses followed previous work on toad poison secretions [9,10,18,[28][29][30][31].…”

Section: Hplc-dad-q-tof-ms/ms Analysismentioning

confidence: 99%

“…Some differences with the Peruvian R. marina are the occurrence of arenobufagin derivatives and a higher number of oxidized compounds in R. horribilis compared with the Amazonian species. Interestingly, most of the compounds reported in the R. horribilis PGS have been previously described from South American Rhinella species [9,10,18] and/or from the crude drug Venenum Bufonis or Ch'an Su from China and Eastern Asia [27,30,31], which exhibit anti-cancer effects in several cancer cell lines [35][36][37][38][39][40]. Based on this, we propose to evaluate the anti-cancer effect of crude PGS of R. horribilis and their fractions A, B, and C.…”

Section: Argininyl Diacids Of Bufadienolidesmentioning

confidence: 99%

The Parotoid Gland Secretion from Peruvian Toad Rhinella horribilis (Wiegmann, 1833): Chemical Composition and Effect on the Proliferation and Migration of Lung Cancer Cells

Schmeda-Hirschmann

Andrade

Soto-Vásquez

et al. 2020

Toxins

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Since Rhinella sp. toads produce bioactive substances, some species have been used in traditional medicine and magical practices by ancient cultures in Peru. During several decades, the Rhinella horribilis toad was confused with the invasive toad Rhinella marina, a species documented with extensive toxinological studies. In contrast, the chemical composition and biological effects of the parotoid gland secretions (PGS) remain still unknown for R. horribilis. In this work, we determine for the first time 55 compounds from the PGS of R. horribilis, which were identified using HPLC-MS/MS. The crude extract inhibited the proliferation of A549 cancer cells with IC50 values of 0.031 ± 0.007 and 0.015 ± 0.001 µg/mL at 24 and 48 h of exposure, respectively. Moreover, it inhibited the clonogenic capacity, increased ROS levels, and prevented the etoposide-induced apoptosis, suggesting that the effect of R. horribilis poison secretion was by cell cycle blocking before of G2/M-phase checkpoint. Fraction B was the most active and strongly inhibited cancer cell migration. Our results indicate that the PGS of R. horribilis are composed of alkaloids, bufadienolides, and argininyl diacids derivatives, inhibiting the proliferation and migration of A549 cells.

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“…As a precious traditional Chinese medicine (TCM), VB has long been used for treating heart failure, arrhythmia, swells, sore throat, pains, cancers and many other diseases ( Liang et al, 2008 ; Pan et al, 2020 ). Extensive natural product studies have indicated that VB contains a high level of bufadienolides and many other constituents like alkaloids, cyclic amides, sterols, polypeptides, proteins, polysaccharides, and organic acids ( Wei et al, 2019 ). Modern pharmacological studies have confirmed that VB exhibited a variety of pharmacological effects, including cardiotonic, antinociceptive, anti-tumor, anesthetic, anti-inflammatory as well as antimicrobial properties ( Wei et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Modern pharmacological studies have confirmed that VB exhibited a variety of pharmacological effects, including cardiotonic, antinociceptive, anti-tumor, anesthetic, anti-inflammatory as well as antimicrobial properties ( Wei et al, 2020 ). Importantly, it is evident that VB exerts a strong cardiac excitatory effect like that of digitalis, and the drug possesses many advantages such as no accumulation, quick-acting and diuretic action ( Wei et al, 2019 ). According to the Chinese Pharmacopoeia (2015 edition), VB is contained in many TCM prescriptions for the treatment of HD, such as Jiuxin Pill and Shexiang Baoxin Pill ( Chinese Pharmacopoeia Commission, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated network pharmacology and molecular docking approaches to reveal the synergistic mechanism of multiple components in Venenum Bufonis for ameliorating heart failure

Ren

Luo

Pan

et al. 2020

PeerJ

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Venenum Bufonis (VB), also called Chan Su in China, has been extensively used as a traditional Chinese medicine (TCM) for treating heart failure (HF) since ancient time. However, the active components and the potential anti-HF mechanism of VB remain unclear. In the current study, the major absorbed components and metabolites of VB after oral administration in rats were first collected from literatures. A total of 17 prototypes and 25 metabolites were gathered. Next, a feasible network-based pharmacological approach was developed and employed to explore the therapeutic mechanism of VB on HF based on the collected constituents. In total, 158 main targets were screened out and considered as effective players in ameliorating HF. Then, the VB components–main HF putative targets–main pathways network was established, clarifying the underlying biological process of VB on HF. More importantly, the main hubs were found to be highly enriched in adrenergic signalling in cardio-myocytes. After verified by molecular docking studies, four key targets (ATP1A1, GNAS, MAPK1 and PRKCA) and three potential active leading compounds (bufotalin, cinobufaginol and 19-oxo-bufalin) were identified, which may play critical roles in cardiac muscle contraction. This study demonstrated that the integrated strategy based on network pharmacology and molecular docking was helpful to uncover the synergistic mechanism of multiple constituents in TCM.

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Venenum bufonis: An overview of its traditional use, natural product chemistry, pharmacology, pharmacokinetics and toxicology

Cited by 44 publications

References 99 publications

Zoopharmacology: A Way to Discover New Cancer Treatments

Zoopharmacology: A Way to Discover New Cancer Treatments

The Parotoid Gland Secretion from Peruvian Toad Rhinella horribilis (Wiegmann, 1833): Chemical Composition and Effect on the Proliferation and Migration of Lung Cancer Cells

Integrated network pharmacology and molecular docking approaches to reveal the synergistic mechanism of multiple components in Venenum Bufonis for ameliorating heart failure

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