Ventilatory acclimatization to hypoxia in mice: Methodological considerations

Ivy, Catherine M.; Scott, Graham R.

doi:10.1016/j.resp.2016.10.012

Cited by 29 publications

(27 citation statements)

References 48 publications

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“…). This hypoxia acclimation response of lowlanders is typical of VAH, a well‐known process that occurs in domestic house mice, rats, guinea‐pigs and humans . Hypoxia acclimation had relatively few effects on breathing and pulmonary gas exchange in highland deer mice (Fig.…”

Section: Discussionmentioning

confidence: 76%

“…This resulted in four treatment groups: normoxia‐acclimated lowlanders ( n = 14), normoxia‐acclimated highlanders ( n = 9), hypoxia‐acclimated lowlanders ( n = 15) and hypoxia‐acclimated highlanders ( n = 9). Specially designed hypobaric chambers were used for hypoxia acclimation, as previously described . Mice were temporarily returned to normobaric conditions twice per week for <20 min for cage cleaning.…”

Section: Methodsmentioning

confidence: 99%

“…Breathing and metabolism were determined during the last 10 min at each

P_{{normalO}_{2}}

, as previously described . Incurrent and excurrent air flows were subsampled at 200 mL min −1 ; incurrent air was continuously measured for O 2 fraction (FC‐10; Sable Systems), and excurrent air was analysed for water vapour (RH‐300; Sable Systems), dried with pre‐baked drierite and analysed for O 2 and CO 2 fraction (FC‐10 and CA‐10; Sable Systems).…”

Section: Methodsmentioning

confidence: 99%

“…Breathing frequency and tidal volume were measured from changes in flow across a pneumotachograph in the plethysmograph wall (which arise from the warming and humidifying of the air as it is inspired by the animal), detected using a differential pressure transducer (Validyne DP45; Cancoppas, Mississauga, ON, Canada). Tidal volume was calculated using established equations assuming a constant rate of decline in T b with declining

P_{{normalO}_{2}}

– an assumption that we have previously shown to result in similar tidal volumes to those calculated using direct T b measurements at each

P_{{normalO}_{2}}

– and is expressed at STP. Total ventilation was determined as the product of breathing frequency and tidal volume, air convection requirement was calculated by dividing total ventilation by

{\dot{V}}_{{normalO}_{2}}

, and pulmonary O 2 extraction (%) was calculated as

{\dot{V}}_{{normalO}_{2}}

divided by the product of total ventilation and inspired

P_{{normalO}_{2}}

.…”

Section: Methodsmentioning

confidence: 99%

“…Specially designed hypobaric chambers were used for hypoxia acclimation, as previously described. 28,36 Mice were temporarily returned to normobaric conditions twice per week for <20 min for cage cleaning. After 18-20 weeks of acclimation, mice were subjected to ventilatory experiments.…”

Section: Acclimation Treatmentsmentioning

confidence: 99%

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Control of breathing and ventilatory acclimatization to hypoxia in deer mice native to high altitudes

Ivy

Scott

2017

Acta Physiologica

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Section: Discussionmentioning

confidence: 76%

Section: Methodsmentioning

confidence: 99%

“…Breathing and metabolism were determined during the last 10 min at each

P_{{normalO}_{2}}

Section: Methodsmentioning

confidence: 99%

P_{{normalO}_{2}}

– an assumption that we have previously shown to result in similar tidal volumes to those calculated using direct T b measurements at each

P_{{normalO}_{2}}

– and is expressed at STP. Total ventilation was determined as the product of breathing frequency and tidal volume, air convection requirement was calculated by dividing total ventilation by

{\dot{V}}_{{normalO}_{2}}

, and pulmonary O 2 extraction (%) was calculated as

{\dot{V}}_{{normalO}_{2}}

divided by the product of total ventilation and inspired

P_{{normalO}_{2}}

.…”

Section: Methodsmentioning

confidence: 99%

Section: Acclimation Treatmentsmentioning

confidence: 99%

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Control of breathing and ventilatory acclimatization to hypoxia in deer mice native to high altitudes

Ivy

Scott

2017

Acta Physiologica

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Systemic Oxygen Transport with Rest, Exercise, and Hypoxia: A Comparison of Humans, Rats, and Mice

González

Kuwahira

2018

Comprehensive Physiology

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The objective of this article is to compare and contrast the known characteristics of the systemic O transport of humans, rats, and mice at rest and during exercise in normoxia and hypoxia. This analysis should help understand when rodent O transport findings can-and cannot-be applied to human responses to similar conditions. The O -transport system was analyzed as composed of four linked conductances: ventilation, alveolo-capillary diffusion, circulatory convection, and tissue capillary-cell diffusion. While the mechanisms of O transport are similar in the three species, the quantitative differences are naturally large. There are abundant data on total O consumption and on ventilatory and pulmonary diffusive conductances under resting conditions in the three species; however, there is much less available information on pulmonary gas exchange, circulatory O convection, and tissue O diffusion in mice. The scarcity of data largely derives from the difficulty of obtaining blood samples in these small animals and highlights the need for additional research in this area. In spite of the large quantitative differences in absolute and mass-specific O flux, available evidence indicates that resting alveolar and arterial and venous blood PO values under normoxia are similar in the three species. Additionally, at least in rats, alveolar and arterial blood PO under hypoxia and exercise remain closer to the resting values than those observed in humans. This is achieved by a greater ventilatory response, coupled with a closer value of arterial to alveolar PO , suggesting a greater efficacy of gas exchange in the rats. © 2018 American Physiological Society. Compr Physiol 8:1537-1573, 2018.

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Characterizing the influence of chronic hypobaric hypoxia on diaphragmatic myofilament contractile function and phosphorylation in high-altitude deer mice and low-altitude white-footed mice

et al. 2019

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Ventilatory acclimatization to hypoxia in mice: Methodological considerations

Cited by 29 publications

References 48 publications

Control of breathing and ventilatory acclimatization to hypoxia in deer mice native to high altitudes

Control of breathing and ventilatory acclimatization to hypoxia in deer mice native to high altitudes

Systemic Oxygen Transport with Rest, Exercise, and Hypoxia: A Comparison of Humans, Rats, and Mice

Characterizing the influence of chronic hypobaric hypoxia on diaphragmatic myofilament contractile function and phosphorylation in high-altitude deer mice and low-altitude white-footed mice

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