Verificação da viabilidade do transplante autógeno de testículo no omento e na parede abdominal em ratos

Nunes, Sergio Ibañez; Fernandes, Carolaine Bitencourt Ferreira; Rezende, Dilermando Fazito de; Bernardes, Jonatas Barros de Guimarães; Silva, Marcus Vinicius de Paula da

doi:10.1590/s0100-69912013000100011

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…This operation technique has many benefits, such as complete anastomosis through ligation on only one occasion, less bleeding from anastomosis sites, shorter ischemic time of the graft, and improved success rate compared with orthotopic transplantation using the general suture technique. Recently, Nunes et al attempted to transplant a whole testis into the greater omentum without vascular anastomosis in rats with resultant failure of graft survival [18]. Therefore, we performed heterotopic testis transplantation (HTT) with blood vessel anastomosis in the present study.…”

Section: Introductionmentioning

confidence: 97%

Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats

Hatayama

Hirai

et al. 2017

PLoS ONE

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Transplantation of testicular cells and tissues has been studied for the investigation of immunology of the testis, which is an immunologically privileged organ. However, reports of transplant of the testis at organ level have been extremely limited because of technical difficulties of the orthotopic testis transplantation (OTT) in experimental animals. In the present study, we developed a new and simple model of the heterotopic testis transplantation (HTT), which is donor testis transplantation into the cervical region of recipients, in a syngeneic model in rats [donor Lewis (LEW) graft to LEW recipient]. The duration of HTT was significantly shorter and success rate higher than that of OTT. To histologically evaluate HTT, the local immune responses were compared among the syngeneic model, an acute rejection allogeneic model [donor Augustus Copenhagen Irish (ACI) graft to LEW recipient] and a chronic rejection allogeneic model (donor F344 graft to LEW recipient) at postoperative day 3. We found that allogeneic ACI grafts resulted in mild and not severe orchitic lesions, whereas immune responses of allogeneic F344 grafts seemed intact and were not significantly different from those of syngeneic LEW grafts. These results suggest that our new operative procedure will be useful in future for the investigation of the testicular immunology.

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Section: Introductionmentioning

confidence: 97%

Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats

Hatayama

Hirai

et al. 2017

PLoS ONE

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Regeneration of Leydig cells in ectopically autografted adult mouse testes

et al. 2015

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Ectopic autografting of testis tissue is a promising approach for studying testicular development, male germline preservation and restoration of male fertility. In this study, we examined the fate of various testicular cells in adult mouse testes following ectopic autografting at 1, 2, 4 and 8 weeks post grafting. Histological examination showed no evidence of re-establishment of spermatogenesis in autografts, and progressive degeneration of seminiferous tubules was detected. Expression of germ cell-specific proteins such as POU5F1, DAZL, TNP1, TNP2, PRM1 and PRM2 revealed that, although proliferating and differentiating spermatogenic germ cells such as spermatogonia, spermatocytes and spermatids could survive in autografts until 4 weeks, only terminally differentiated germ cells such as sperm persisted in autografts until 8 weeks. The presence of Sertoli and peritubular myoid cells, as indicated by expression of WT1 and ACTA2 proteins, respectively, was evident in the autografts until 8 weeks. Interestingly, seminal vesicle weight and serum testosterone level were restored in autografted mice by 8 weeks post grafting. The expression of Leydig cell-specific proteins such as CYP11A1, HSD3B2 and LHCGR showed revival of Leydig cell (LC) populations in autografts over time since grafting. Elevated expression of PDGFRA, LIF, DHH and NEFH in autografts indicated de novo regeneration of LC populations. Autografted adult testis can be used as a model for investigating Leydig cell regeneration, steroidogenesis and regulation of the intrinsic factors involved in Leydig cell development. The success of this rodent model can have therapeutic applications for adult human males undergoing sterilizing cancer therapy.

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Verificação da viabilidade do transplante autógeno de testículo no omento e na parede abdominal em ratos

Abstract: autologous transplantation of testis to the greater omentum and abdominal wall without vascular anastomosis is not viable in rats.

Cited by 2 publications

References 17 publications

Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats

Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats

Regeneration of Leydig cells in ectopically autografted adult mouse testes

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