Vero cytotoxin production and HeLa cell adherence of enteropathogenic Escherichia coli of infantile diarrhoea in Iran

Abstract

A total of 70 enteropathogenic Escherichia coli (EPEC) strains belonging to 11 serogroups, isolated from infantile diarrhoea in Tehran, Iran, were tested for the production of verocytotoxin (VT), enterotoxin, and also for their adherence to HeLa cells. In total 55 (78.5%) strains were either VT (32 strains) or enterotoxin (23 strains) producers, and of these 8 strains produced both VT and enterotoxins. 57 (81.4%) strains showed either Localized (LA) or Diffuse adherence (DA) or both types of adhesion (LA/DA) on HeLa cells, with strains showing LA/DA in the same preparations being dominant (32 strains), followed by those showing LA (14 strains) and DA (11 strains). Among adherent EPEC, 26 (37.1%) strains belonging to the serogroups 020, 086, 0119, 0125, 0126, 0127 and 0128 also produced VT. These findings suggest that production of VT and enterotoxin is an important factor in the pathogenesis of EPEC diarrhoea in Iran and that the combination of adherence and production of toxins is a common feature of EPEC strains which cause diarrhoea in this country.

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

“…Many isolates of EPEC serogroups 026 and 0111 produce SLTs, produce A/E lesions both in vitro (114) and in vivo (203), and are classed as EHEC rather than EPEC (86,124,150,183). Some E. coli isolates belonging to EPEC serogroups such as 055, 0119, and 0126 also produce SLTs (23,34,59,109,190,191,215). EPEC isolates of most other classical serogroups do not hybridize with probes for SLT-I or SLT-II genes, and although they are reported to be cytotoxic, the activity is low (38,133,151) and the cytotoxin is active on cell lines that are unaffected by Shiga toxin (34,190).…”

Risk Factors or Predictors of Developing Ciprofloxacin, Trimethoprim/Sulfamethoxazole and Third Generation Cephalosporin Resistance in Escherichia Coli Infections: A Systematic Review and Meta-Analysis

“…E. coli is responsible for 70% of UTIs in diabetic patients. 47 The major reason for the common infection is high glucose levels which perhaps suppress the immune system by diminishing neutrophil and antioxidant function. 48 Additionally, glucose helps E. coli for fast replication.…”

Adhesion and its role in the virulence of enteropathogenic Escherichia coli.

“…Many isolates of EPEC serogroups 026 and 0111 produce SLTs, produce A/E lesions both in vitro (114) and in vivo (203), and are classed as EHEC rather than EPEC (86,124,150,183). Some E. coli isolates belonging to EPEC serogroups such as 055, 0119, and 0126 also produce SLTs (23, 34,59,109,190,191,215). EPEC isolates of most other classical serogroups do not hybridize with probes for SLT-I or SLT-II genes, and although they are reported to be cytotoxic, the activity is low (38, 133, 151) and the cytotoxin is active on cell lines that are unaffected by Shiga toxin (34,190).…”