Versatile High-Performance Electrochemiluminescence ELISA Platform Based on a Gold Nanocluster Probe

Peng, Hua-Ping; Huang, Zhongnan; Wu, Weihua; Liu, Mingkai; Huang, Kai-Yuan; Yu, Yang; Deng, Hao‐Hua; Xia, Xing‐Hua; Chen, Wei

doi:10.1021/acsami.9b08819

Cited by 68 publications

(35 citation statements)

References 50 publications

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“…When this assay was applied to a commercial kit to detect Aβ 42 , it enhanced the detection sensitivity by a factor of more than 10. Electrochemiluminescence ELISA combined with an Au nanocluster technology was developed, and the LOD for tumor necrosis factor-α (TNF-α) was two orders lower than that using a conventional ELISA [ 52 ].…”

Section: Recent Advances In Modified Elisa Methods To Realize Ultrasensitive Diagnosismentioning

confidence: 99%

Modified ELISA for Ultrasensitive Diagnosis

Tsurusawa

Chang

Namba

et al. 2021

JCM

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An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term ‘ultrasensitive’. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestyle-related diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.

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Section: Recent Advances In Modified Elisa Methods To Realize Ultrasensitive Diagnosismentioning

confidence: 99%

Modified ELISA for Ultrasensitive Diagnosis

Tsurusawa

Chang

Namba

et al. 2021

JCM

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“…Improvement on the ELISA method also has been made with a fluorogenic reporter [ 68 ], polymerase chain reaction (PCR) [ 69 ] and electrochemiluminescence reporters [ 70 ] to improve signal generation. The recent major advancement of ELISA was the manipulation of gold nanoparticles, as chromogenic reporters that greatly improve the sensitivity of detection.…”

Section: Detection Of Salmonellamentioning

confidence: 99%

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection

et al. 2021

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Large-scale food-borne outbreaks caused by Salmonella are rarely seen nowadays, thanks to the advanced nature of the medical system. However, small, localised outbreaks in certain regions still exist and could possess a huge threat to the public health if eradication measure is not initiated. This review discusses the progress of Salmonella detection approaches covering their basic principles, characteristics, applications, and performances. Conventional Salmonella detection is usually performed using a culture-based method, which is time-consuming, labour intensive, and unsuitable for on-site testing and high-throughput analysis. To date, there are many detection methods with a unique detection system available for Salmonella detection utilising immunological-based techniques, molecular-based techniques, mass spectrometry, spectroscopy, optical phenotyping, and biosensor methods. The electrochemical biosensor has growing interest in Salmonella detection mainly due to its excellent sensitivity, rapidity, and portability. The use of a highly specific bioreceptor, such as aptamers, and the application of nanomaterials are contributing factors to these excellent characteristics. Furthermore, insight on the types of biorecognition elements, the principles of electrochemical transduction elements, and the miniaturisation potential of electrochemical biosensors are discussed.

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“…The double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is preferentially used to detect pathogenic bacteria [1], viruses [2], and biomarkers in samples for rapid and accurate diagnosis [3]. For example, many commercial double-antibody sandwich ELISAs have been developed for the diagnosis of human and animal diseases [4,5].…”

Section: Introductionmentioning

confidence: 99%

Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Zhu

et al. 2020

Preprint

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Background Traditional sandwich enzyme-linked immunosorbent assay (ELISA) using polyclonal and monoclonal antibodies as reagents presents several drawbacks, including limited amounts, difficulty in permanent storage, and required use of a secondary antibody. Nanobodies can be easily expressed with different systems and fused with several tags in their tertiary structure by recombinant technology, thus offering an effective detection method for diagnostic purposes. Recently, the fenobody (ferritin-fused nanobody) and RANbody (nanobody-fused reporter) have been designed and derived from the nanobody for developing the diagnostic immunoassays. However, there was no report about developing the sandwich ELISA using the fenobody and RANbody as pairing reagents. Results A platform for developing a sandwich ELISA utilizing fenobody as the capture antibody and RANbody as the detection antibody was firstly designed in the study. Newcastle disease virus (NDV) was selected as the antigen, from which 13 NDV-specific nanobodies were screened from an immunized Bactrian camel. Then, 5 nanobodies were selected to produce fenobodies and RANbodies. The best pairing of fenobodies (NDV-fenobody-4, 800 ng/well) and RANbodies (NDV-RANbody-49, 1:10) was determined to develop the sandwich ELISA for detecting NDV. The detection limits of the assay were determined to be 2 2 of hemagglutination (HA) titers and 10 ng of purified NDV particles. Compared with two commercial assays, the developed assay shows higher sensitivity and specificity. Meanwhile, it exhibits 98.7% agreement with the HA test and can detect the reference NDV strains belonging to Class II but not Class I. Conclusions In the presented study, the 13 anti-NDV nanobodies binding the NDV particles were first produced. Then, for the first time, the sandwich ELISA to detect the NDV in the different samples has been developed using the fenobody and RANbody as reagents derived from the nanobodies. Considering the rapidly increasing generation of nanobodies, the platform can reduce the cost of production for the sandwich ELISA and be universally used to develop assays for detecting other antigens.

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Versatile High-Performance Electrochemiluminescence ELISA Platform Based on a Gold Nanocluster Probe

Cited by 68 publications

References 50 publications

Modified ELISA for Ultrasensitive Diagnosis

Modified ELISA for Ultrasensitive Diagnosis

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection

Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

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