Versatile online <scp>SPE</scp>–<scp>HPLC</scp> method for the analysis of Irinotecan and its clinically relevant metabolites in biomaterials

Prijovich, Zeljko M.; Burnouf, Pierre-Alain; Roffler, Steve R.

doi:10.1002/jssc.201301191

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…The plasma was separated from the red blood cells by centrifugation at 2500 × g for 3 min. Plasma samples containing liposomes were destabilized with 1% Triton X-100 to lyse the liposomes and diluted 1:1 (vol:vol) with acetonitrile:methanol:0.2 M trichloroacetic acid (2:2:1, vol:vol) to precipitate proteins 57 and centrifuged at 20,000 × g for 5 min at 4 °C. The supernatant was directly injected into a C18 HPLC column with fluorescent detection at 370/460 nm (Ex/Em).…”

Section: Methodsmentioning

confidence: 99%

Reversible glycosidic switch for secure delivery of molecular nanocargos

Burnouf

Leu

et al. 2018

Nat Commun

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Therapeutic drugs can leak from nanocarriers before reaching their cellular targets. Here we describe the concept of a chemical switch which responds to environmental conditions to alternate between a lipid-soluble state for efficient cargo loading and a water-soluble state for stable retention of cargos inside liposomes. A cue-responsive trigger allows release of the molecular cargo at specific cellular sites. We demonstrate the utility of a specific glycosidic switch for encapsulation of potent anticancer drugs and fluorescent compounds. Stable retention of drugs in liposomes allowed generation of high tumor/blood ratios of parental drug in tumors after enzymatic hydrolysis of the glycosidic switch in the lysosomes of cancer cells. Glycosidic switch liposomes could cure mice bearing human breast cancer tumors without significant weight loss. The chemical switch represents a general method to load and retain cargos inside liposomes, thereby offering new perspectives in engineering safe and effective liposomes for therapy and imaging.

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Section: Methodsmentioning

confidence: 99%

Reversible glycosidic switch for secure delivery of molecular nanocargos

Burnouf

Leu

et al. 2018

Nat Commun

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“…SPE is a common approach to the preparation of complicated samples, which can be performed on‐line by directly connecting to the LC system. Many different hyphenated SPE–LC on‐line systems have been developed, such as SPE–HPLC , SPE–LC–MS/MS , SPE–LC–TOF‐MS , and so on. In our previous study, an automatic analysis on‐line system coupling SPE with UHPLC–MS/MS using a dual‐dilution strategy was developed .…”

Section: Introductionmentioning

confidence: 99%

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Zhong

Shen

Liu

et al. 2016

J of Separation Science

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An automatic on-line solid-phase extraction with ultra-high performance liquid chromatography and tandem mass spectrometry method was developed for the simultaneous determination of ten antipsychotics in human plasma. The plasma sample after filtration was injected directly into the system without any pretreatment. A Shim-pack MAYI-C8 (G) column was used as a solid-phase extraction column, and all the analytes were separated on a Shim-pack XR-ODS III column with a mobile phase consisting of 0.1% v/v formic acid in water with 5 mM ammonium acetate and acetonitrile. The method features were systematically investigated, including extraction conditions, desorption conditions, the equilibration solution, the valve switching time, and the dilution for column-head stacking. Under the optimized conditions, the whole analysis procedure took only 10 min. The limits of quantitation were in the range of 0.00321-2.75 μg/L and the recoveries ranged from 75.9 to 122%. Compared with the off-line ultra-high performance liquid chromatography and the reported methods, this validated on-line method showed significant advantages such as minimal pretreatment, shortest analysis time, and highest sensitivity. The results indicated that this automatic on-line method was rapid, sensitive, and reliable for the determination of antipsychotics in plasma and could be extended to other target analytes in biological samples.

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“…Nowadays, common sample pretreatment techniques for monoamine acidic metabolites include LLE, SPE, and SPME and microdialysis. Among these pretreatment techniques, SPE and SPME have been considered as excellent online sample pretreatment techniques to decrease the interference of matrix and enrich target compounds. SPME is a solvent‐free and environmentally friendly sample pretreatment method, but its extraction capacity is limited due to the limit of the volume of extraction phase on the surface of coating.…”

Section: Introductionmentioning

confidence: 99%

Acrylamide‐functionalized graphene micro‐solid‐phase extraction coupled to high‐performance liquid chromatography for the online analysis of trace monoamine acidic metabolites in biological samples

Yang

et al. 2015

J of Separation Science

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Monoamine acidic metabolites in biological samples are essential biomarkers for the diagnosis of neurological disorders. In this work, acrylamide-functionalized graphene adsorbent was successfully synthesized by a chemical functionalization method and was packed in a homemade polyether ether ketone micro column as a micro-solid-phase extraction unit. This micro-solid-phase extraction unit was directly coupled to high-performance liquid chromatography to form an online system for the separation and analysis of three monoamine acidic metabolites including homovanillic acid, 5-hydroxyindole-3-acetic acid, and 3,4-dihydroxyphenylacetic acid in human urine and plasma. The online system showed high stability, permeability, and adsorption capacity toward target metabolites. The saturated extraction amount of this online system was 213.1, 107.0, and 153.4 ng for homovanillic acid, 5-hydroxyindole-3-acetic acid, and 3,4-dihydroxyphenylacetic acid, respectively. Excellent detection limits were achieved in the range of 0.08-0.25 μg/L with good linearity and reproducibility. It was interesting that three targets in urine and plasma could be actually quantified to be 0.94-3.93 μg/L in plasma and 7.15-19.38 μg/L in urine. Good recoveries were achieved as 84.8-101.4% for urine and 77.8-95.1% for plasma with the intra- and interday relative standard deviations less than 9.3 and 10.3%, respectively. This method shows great potential for online analysis of trace monoamine acidic metabolites in biological samples.

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Versatile online SPE–HPLC method for the analysis of Irinotecan and its clinically relevant metabolites in biomaterials

Cited by 10 publications

References 40 publications

Reversible glycosidic switch for secure delivery of molecular nanocargos

Reversible glycosidic switch for secure delivery of molecular nanocargos

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Acrylamide‐functionalized graphene micro‐solid‐phase extraction coupled to high‐performance liquid chromatography for the online analysis of trace monoamine acidic metabolites in biological samples

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