Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins

Vehus, Tore; Røberg-Larsen, Hanne; Waaler, Jo; Aslaksen, Sigrid; Krauß, Stefan; Wilson, Steven Ray; Lundanes, Elsa

doi:10.1038/srep37507

Cited by 38 publications

(23 citation statements)

References 47 publications

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“…In the field of cancer cell biology, activation of oncogenic signalling pathways can be assessed by measuring changes in the post-translational modifications of pathway proteins, as well as with high throughput transcriptomic and proteomic approaches and other proximity based assays. Sterols in tissue or cells lines can now be directly detected in amounts as low as 25 attograms using spectrometry based methods [59].…”

Section: Tools For Studying Phytosterols In Membranesmentioning

confidence: 99%

Exploring the biophysical properties of phytosterols in the plasma membrane for novel cancer prevention strategies

et al. 2018

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Cancer is a global problem with no sign that incidences are reducing. The great costs associated with curing cancer, through developing novel treatments and applying patented therapies, is an increasing burden to developed and developing nations alike. These financial and societal problems will be alleviated by research efforts into prevention, or treatments that utilise off-patent or repurposed agents. Phytosterols are natural components of the diet found in an array of seeds, nuts and vegetables and have been added to several consumer food products for the management of cardio-vascular disease through their ability to lower LDL-cholesterol levels. In this review, we provide a connected view between the fields of structural biophysics and cellular and molecular biology to evaluate the growing evidence that phytosterols impair oncogenic pathways in a range of cancer types. The current state of understanding of how phytosterols alter the biophysical properties of plasma membrane is described, and the potential for phytosterols to be repurposed from cardio-vascular to oncology therapeutics. Through an overview of the types of biophysical and molecular biology experiments that have been performed to date, this review informs the reader of the molecular and biophysical mechanisms through which phytosterols could have anti-cancer properties via their interactions with the plasma cell membrane. We also outline emerging and under-explored areas such as computational modelling, improved biomimetic membranes and ex vivo tissue evaluation. Focus of future research in these areas should improve understanding, not just of phytosterols in cancer cell biology but also to give insights into the interaction between the plasma membrane and the genome. These fields are increasingly providing meaningful biological and clinical data but iterative experiments between molecular biology assays, biosynthetic membrane studies and computational membrane modelling improve and refine our understanding of the role of different sterol components of the plasma membrane.

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Section: Tools For Studying Phytosterols In Membranesmentioning

confidence: 99%

Exploring the biophysical properties of phytosterols in the plasma membrane for novel cancer prevention strategies

et al. 2018

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“…sample capacity is lower on these systems compared to conventional larger ID systems. Hence, using strong cation exchange (SCX) columns on-line [49] or high capacity solid phase extraction columns (poly-styrene-octadecene-divinylbenzene, PS-OD-DVB [50]) often needed to take full advantage of the increased sensitivity of such downscaled systems [51].…”

Section: Downscaled Lc Systems: Enhanced Sensitivity With Esi-msmentioning

confidence: 99%

“…Databases [29,32] Quantification [25,54,55,58,60] Liquid chromatography [2,3,13,42,49,51] Mass spectrometry [10,11,35,56] Data treatment [18-21, 30, 31] Table 3. A selection of studies referenced in the chapter focused on databases, quantification, liquid chromatography, mass spectrometry and data treatment.…”

Section: Some Referencesmentioning

confidence: 99%

Performing Quantitative Determination of Low-Abundant Proteins by Targeted Mass Spectrometry Liquid Chromatography

Vehus¹

2017

Mass Spectrometry

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Mass spectrometry coupled to nanoliquid chromatography aims to be an alternative to antibody-based determination of low-abundant proteins. High-resolution mass spectrometers, plug-and-play systems and pumps have been developed for this purpose. Important aspects of approaches are limit of detection, specificity, variability and cost.In this chapter, the most recent literature (from 2008) has been reviewed and a checklist/workflow for targeted proteomics is presented with special focus on low-abundant proteins in complex matrices. The chapter is intended to serve as a starting point for low-abundant target determination and highlights some of the most central studies in this field.

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“…OT têm um consumo extremamente baixo de solventes, e permite maior sensibilidade ao usar espectrometria de massas por ionização por electrospray (ESI-MS) (93). OT colunas têm recebido atenção devido à sua bem sucedida cromatografia de compostos de elevada massa molecular, tais como proteínas e peptídeos com alta capacidade de pico em sistemas de alta sensibilidade nano-LC tandem MS (MS / MS) (32,92,94,95,96,97). No entanto, as colunas do tipo OT estão associadas a uma fraca retenção e é praticamente não favorável para a análise de moléculas pequenas, o que está muito mais associado a colunas maiores e de alta capacidade.…”

Section: Colunas Plotunclassified

“…Para permitir a injeção de μL de amostras (isto é, capacidade superior) em colunas OT, pode ser utilizada a SPE em linha. Apenas um número limitado de estudos exploraram variantes de coluna SPE para acoplamento OT (32,33,92,94,95,96,97,100,101).…”

Section: Colunas Plotunclassified

Novas estratégias de preparo de amostras complexas para a determinação de sulfonamidas

Silva¹

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AGRADECIMENTOPrimeiramente a Deus.Aos meus pais, que diante das dificuldades da vida me proporcionaram a oportunidade de estudar sem medir esforços, pelo amor, puxão de orelha, pelo colo, ombro e por ser minha torcida organizada, obrigada é muito pouco! Ao meu irmão por ser o meu porto seguro sempre dizendo qualquer coisa eu estou aqui, obrigada pelo ombro, força e apoio incondicional! Ao Professor Dr. Fernando M. Lanças por me receber no laboratório de cromatografia, por me capacitar tecnicamente, pelo exemplo de profissional dedicado e eficiente, pela atenção, conselhos e incentivos ao longo desses anos, muito obrigada! Ao Alcimar (in memoria) pelo apoio e ensinamentos.Ao Professor Dr. Álvaro por me motivar através de uma conversa informal a trabalhar com líquidos iônicos e além disso, pelo exemplo de profissional, dedicação e humildade, obrigada.Ao Grupo de Cromatografia que tornaram a minha vida científica muito mais prazerosa não só pelos cafés, bolos e churrascos, mas pela companhia e inúmeras risadas, muito obrigada. Á Odete que agiu como meio mãe, amiga nestes anos e pelas inúmeras risadas, obrigada! Á Elaine pelo exemplo de dedicação, organização e pelas conversas, obrigada.Ao Guilherme que sempre foi solícito e disponível para discutir e dividir seu conhecimento, e lógico pelo cafezinho e pela companhia, obrigada.Ao grupo de Bioanalítica da UiO pelo conhecimento adquirido e por tornarem os dias frios um pouco mais amenos para uma brasileira, obrigada! E por fim, agradecer aqueles que cruzaram meu caminho e foram de extrema importância para chegar até aqui, muito obrigada Carlinhos, Maurinha, Maraíssa, Rodrigo, Raquel, Scarlet, Andreinha, Tanare, Pipe, Fumes, Arley, Camilinha, Van, Dani, Paty, Carol, Gabi, Jiraia, Lari, Naty, Stella, Josi e Kenia.O sucesso nasce do querer, da determinação e persistência em se chegar a um objetivo. Mesmo não atingindo o alvo, quem busca e vence obstáculos, no mínimo fará coisas admiráveis. José de Alencar RESUMO ABSTRACTNowadays health regulatory agencies are focusing on the presence of residues of antimicrobials, pesticides among other that are harmful to the environment, human and animal health that are found on food, river water among others, as example, the residues of sulfonamides (SAs) in bovine milk. The quantification of these compounds in complex matrices requires the sample preparation, extraction and preconcentration of the analytes, besides the elimination of the interferents for the development of chromatographic methods high sensitivity and analytical selectivity. Therefore, it is advantageous to use simple, fast methods that exhibit high selectivity, low reagent consumption, high sensitivity, higher analytical precision and automation of analyzes. In this context, this study explores one of the most employed sample preparation techniques, solid phase extraction (SPE) on automatization approach, the on-line SPE.Recently Compared to the use of monolithic / particulate SPE columns, SPE-OTLC mPLOT significantly improved the preconcentration and peak forms of ...

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Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins

Cited by 38 publications

References 47 publications

Exploring the biophysical properties of phytosterols in the plasma membrane for novel cancer prevention strategies

Exploring the biophysical properties of phytosterols in the plasma membrane for novel cancer prevention strategies

Performing Quantitative Determination of Low-Abundant Proteins by Targeted Mass Spectrometry Liquid Chromatography

Novas estratégias de preparo de amostras complexas para a determinação de sulfonamidas

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