Vertical distribution and community composition of anammox bacteria in sediments of a eutrophic shallow lake

Qin, Hongyi; Han, Cheng; Jin, Zhiwei; Wu, Lianghuan; Deng, Huan; Zhu, Guangwei; Zhong, Wang

doi:10.1111/jam.13758

Cited by 14 publications

(5 citation statements)

References 54 publications

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“…Abundance of anammox bacteria based on the hzo gene quantification was positively correlated with the anammox rates, which had a negative correlation with both nosZ I and nosZ II gene abundance (Table ). No significant difference between the hzo gene abundance in the near‐surface and deeper sediments was observed in this study, which differs from the depth profiles of anammox bacterial abundance found in shallow, eutrophic Lake Taihu in China (Qin et al, ). This contrast might be the result of hydrologic control on fluctuating N substrates and dissolved O 2 concentrations in Ashumet Pond, preventing proliferation of anammox bacteria, which have characteristically slow growth rates (Oshiki et al, ).…”

Section: Discussioncontrasting

confidence: 97%

Seasonal and Spatial Variation in the Location and Reactivity of a Nitrate‐Contaminated Groundwater Discharge Zone in a Lakebed

et al. 2019

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Groundwater discharge delivering anthropogenic N from surrounding watersheds can impact lake nutrient budgets. However, upgradient groundwater processes and changing dynamics in N biogeochemistry at the groundwater‐lake interface are complex. In this study, seasonal water‐level variations in a groundwater flow‐through lake altered discharge patterns of a wastewater‐derived groundwater contaminant plume, thereby affecting biogeochemical processes controlling N transport. Pore water collected 15 cm under the lakebed along transects perpendicular to shore varied from oxic to anoxic with increasing nitrate concentrations (10–75 μM) and corresponding gradients in nitrite and nitrous oxide. Pore water depth profiles of nitrate concentrations and stable isotopic compositions largely reflected upgradient groundwater N sources and N cycle processes, with minor additional nitrate reduction in the near‐surface lakebed sediments. Potential denitrification rates determined in laboratory microcosms were 10–100 times higher in near‐surface sediments (0–5 cm) than in deeper sediments (5–30 cm) and were correlated with sediment carbon content and abundance of denitrification genes (nirS, nosZI, and nosZII). Potential anammox‐driven N2 production was detectable in deeper anoxic sediments. Injection of bromide and nitrite in the lake sediments showed that the highest net nitrite consumption rates were within the top 10 cm. However, short transit times owing to rapid upward pore water velocities (4–5 cm hr−1) limited removal of the contaminant nitrate transiting through the sediments. Results demonstrate that local hydrologic and biogeochemical processes at the point of discharge affect the distribution and discharge rate of N through lakebed sediments, but processes in the upgradient groundwater can be more important for affecting N speciation and concentration.

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Section: Discussioncontrasting

confidence: 97%

Seasonal and Spatial Variation in the Location and Reactivity of a Nitrate‐Contaminated Groundwater Discharge Zone in a Lakebed

et al. 2019

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“…3b). This phenomenon was consistent with the decreases of total bacteria abundance with depth in other sediments (Qin et al 2018). These findings further demonstrated that not all anoxic vertical profiles were active denitrification regions.…”

Section: Resultssupporting

confidence: 90%

Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes

Hong

Shu

et al. 2019

AMB Expr

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The responses of denitrifiers and denitrification ability to dissolved oxygen (DO) concent in different layers of surface lake sediments are still poorly understood. Here, the optimal denitrification condition was constructed based on response surface methodology (RSM) to analyze the denitrification characteristics of surface sediments. The aerobic zone (AEZ), hypoxic zone (HYZ), up-anoxic zone (ANZ-1) and sub-anoxic zone (ANZ-2) were partitioned based on the oxygen contents, and sediments were collected using a customized-designed sub-millimeter scale sampling device. Integrated real-time quantitative PCR, Illumina Miseq-based sequencing and denitrifying enzyme activities analysis revealed that denitrification characteristics varied among different DO layers. Among the four layers, the DNA abundance and RNA expression levels of norB , nirS and nosZ were the highest at the aerobic layer, hypoxic layer and up-axoic layer, respectively. The hypoxia and up-anaerobic layer were the active nitrogen removal layers, since these two layers displayed the highest DNA abundance, RNA expression level and enzyme activities of denitrification functional genes. The abundance of major denitrifying bacteria showed significant differences among layers, with Azoarcus , Pseudogulbenkiania and Rhizobium identified as the main nirS , nirK and nosZ -based denitrifiers. Pearson’s correlation revealed that the response of denitrifiers to environmental factors differed greatly among DO layers. Furthermore, napA showed higher DNA abundance and RNA expression level in the aerobic and hypoxic layers than anaerobic layers, indicating that aerobic denitrifiers might play important roles at these layers. Electronic supplementary material The online version of this article (10.1186/s13568-019-0855-9) contains supplementary material, which is available to authorized users.

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“…Amplicons were pooled in equimolar amounts and paired-end sequenced on the Illumina MiSeq platform (Illumina) at Majorbio Bio-Pharm Technology Co. Ltd. Raw fastq files were demultiplexed, quality-filtered by Trimmomatic and merged by FLASH according to the criteria, detailed in Qin et al (2018). Operational taxonomic units (OTUs) were clustered based on a 97% identity cut-off using UPARSE (version 7.1 http://drive5.com/upars e/), and chimeric sequences were identified and removed using UCHIME.…”

Section: Microbial Community Structurementioning

confidence: 99%

Meiofauna promotes litter decomposition in stream ecosystems depending on leaf species

Wang

Lin

et al. 2020

Ecology and Evolution

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Litter decomposition, a fundamental process of nutrient cycling and energy flow in freshwater ecosystems, is driven by a diverse array of decomposers. As an important component of the heterotrophic food web, meiofauna can provide a trophic link between leaf‐associated microbes (i.e., bacteria and fungi)/plant detritus and macroinvertebrates, though their contribution to litter decomposition is not well understood. To investigate the role of different decomposer communities in litter decomposition, especially meiofauna, we compared the litter decomposition of three leaf species with different lignin to nitrogen ratios in litter bags with different mesh sizes (0.05, 0.25, and 2 mm) in a forested stream, in China for 78 days. The meiofauna significantly enhanced the decomposition of leaves of high‐and medium‐ quality, while decreasing (negative effect) or increasing (positive effect) the fungal biomass and diversity. Macrofauna and meiofauna together contributed to the decomposition of low‐quality leaf species. The presence of meiofauna and macrofauna triggered different aspects of the microbial community, with their effects on litter decomposition varying as a function of leaf quality. This study reveals that the meiofauna increased the trophic complexity and modulated their interactions with microbes, highlighting the important yet underestimated role of meiofauna in detritus‐based ecosystems.

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Vertical distribution and community composition of anammox bacteria in sediments of a eutrophic shallow lake

Abstract: This is the first study to demonstrate that anammox bacteria displayed the particular distribution in freshwater sediments, which implied a strong response to the anthropogenic eutrophication.

Cited by 14 publications

References 54 publications

Seasonal and Spatial Variation in the Location and Reactivity of a Nitrate‐Contaminated Groundwater Discharge Zone in a Lakebed

Seasonal and Spatial Variation in the Location and Reactivity of a Nitrate‐Contaminated Groundwater Discharge Zone in a Lakebed

Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes

Meiofauna promotes litter decomposition in stream ecosystems depending on leaf species

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