Aim: The purpose of this study is to evaluate the effect of different torque settings of ProTaper Next files on microcrack formation. Materials and methods: Ninety mandibular anterior teeth were used. Fifteen teeth served as control and 75 teeth were divided into three groups according to the torque settings (n=25). In group 1; the root canals were instrumented using ProTaper Next files at a rotational speed of 300rpm and 2.0 Ncm torque. In group 2; the files were used with 3.5 Ncm torque, and in group 3; the files were used with 5.2 Ncm torque. Teeth were horizontally sectioned at 3, 6, and 9 mm and examined with a stereomicroscope. Results: The percentage of total cracked teeth in the 2.0, 3.5, and 5.2 Ncm groups were recorded at 24%, 28%, and 32%, respectively, without any significant difference (p>0.05). Conclusion: The use of ProTaper Next files with high torque settings did not affect the amount of microcrack occurrence. The clinicians may use the rotary systems within different torque values suggested by manufacturers in reliance upon their clinical experiences.