Vertically sheathing laminar flow-based immunoassay using simultaneous diffusion-driven immune reactions

Kurmashev, Amanzhol; Kwon, Soon-Young; Park, Je‐Kyun; Kang, Joo H.

doi:10.1039/c9ra03855h

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…A similar method has been employed for immunoassay of an antigen-antibody interaction in whole blood (Hatch et al 2001). Furthermore, diffusion-based techniques have been employed for the analysis of protein aggregation in antibody preparations (Hawe et al 2011) and in the development of a wash-free ELISA immunoassay (Kurmashev et al 2019). Moreover, the continuous-flow nature of MDS makes it suitable for uninterrupted analysis of PPIs occurring in real time, as demonstrated by continuous observation of insulin aggregation by flow-focussing MDS (Saar et al 2016).…”

Section: Diffusion Analysismentioning

confidence: 99%

Microfluidic approaches for the analysis of protein–protein interactions in solution

et al. 2020

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Exploration and characterisation of the human proteome is a key objective enabling a heightened understanding of biological function, malfunction and pharmaceutical design. Since proteins typically exhibit their behaviour by binding to other proteins, the challenge of probing protein-protein interactions has been the focus of new and improved experimental approaches. Here, we review recently developed microfluidic techniques for the study and quantification of protein–protein interactions. We focus on methodologies that utilise the inherent strength of microfluidics for the control of mass transport on the micron scale, to facilitate surface and membrane-free interrogation and quantification of interacting proteins. Thus, the microfluidic tools described here provide the capability to yield insights on protein–protein interactions under physiological conditions. We first discuss the defining principles of microfluidics, and methods for the analysis of protein–protein interactions that utilise the diffusion-controlled mixing characteristic of fluids at the microscale. We then describe techniques that employ electrophoretic forces to manipulate and fractionate interacting protein systems for their biophysical characterisation, before discussing strategies that use microdroplet compartmentalisation for the analysis of protein interactions. We conclude by highlighting future directions for the field, such as the integration of microfluidic experiments into high-throughput workflows for the investigation of protein interaction networks.

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Section: Diffusion Analysismentioning

confidence: 99%

Microfluidic approaches for the analysis of protein–protein interactions in solution

et al. 2020

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A magnetic bead array-based fluorescence immunoassay platform for robust cancer biomarker analysis in clinical blood samples

Oh,

Han,

Hwang

et al. 2024

Microfluid Nanofluid

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Multiscale mechanisms of reaction-diffusion process in electrode systems: A classical density functional study

Liu

2020

Chemical Engineering Science

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Vertically sheathing laminar flow-based immunoassay using simultaneous diffusion-driven immune reactions

Abstract: Simultaneous infusion of primary and secondary antibodies of different diffusivity into vertical laminar flows enables the improved immune reactions.

Cited by 4 publications

References 35 publications

Microfluidic approaches for the analysis of protein–protein interactions in solution

Microfluidic approaches for the analysis of protein–protein interactions in solution

A magnetic bead array-based fluorescence immunoassay platform for robust cancer biomarker analysis in clinical blood samples

Multiscale mechanisms of reaction-diffusion process in electrode systems: A classical density functional study

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