Very small embryonic-like stem cells as a novel developmental concept and the hierarchy of the stem cell compartment

Ratajczak, Mariusz Z.; Marycz, Krzysztof; Poniewierska-Baran, Agata; Fiedorowicz, Katarzyna; Zbucka-Krętowska, Monika; Moniuszko, Marcin

doi:10.1016/j.advms.2014.08.001

Cited by 44 publications

(38 citation statements)

References 121 publications

(165 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…What is more, AF‐MSCs have the capability of myogenic and neural differentiation as demonstrated in this study. These findings suggest that MSCs from amniotic fluid could occupy the niche in the stem cells hierarchy between pluripotent stem cells having the ability to differentiate into cells from all three germ layers (meso‐, ecto‐ and endoderm) as well as into germline cells and more committed multipotent stem cells able to give rise to two germ layers (Ratajczak et al, ). Our study shows that AF‐MSCs from normal and fetus‐affected gestations did not differ significantly from each other in terms of morphology, phenotypic and pluripotency markers and their differentiation potential.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus‐affected gestations: A focus on myogenic and neural differentiations

Gasiūnienė

Zentelytė

Treigytė

et al. 2019

Cell Biology International

View full text Add to dashboard Cite

Amniotic fluid‐derived mesenchymal stem cells (AF‐MSCs) are autologous to the fetus and represent a potential alternative source for the regenerative medicine and treatment of perinatal disorders. To date, AF‐MSCs differentiation capacity to non‐mesodermal lineages and epigenetic regulation are still poorly characterized. The present study investigated the differentiation potential of AF‐MSCs toward neural‐like cells in comparison to the mesodermal myogenic lineage and assessed epigenetic factors involved in tissue‐specific differentiation. Myogenic and neural differentiation assays were performed by the incubation with specific induction media. Typical MSCs markers were determined by flow cytometry, the expression of lineage‐specific genes, microRNAs and chromatin modifying proteins were examined by RT‐qPCR and Western blot, respectively. AF‐MSCs of normal and fetus‐affected gestations had similar stem cells characteristics and two‐lineage potential, as characterized by cell morphology and the expression of myogenic and neural markers. Two‐lineage differentiation process was associated with the down‐regulation of miR‐17 and miR‐21, the up‐regulation of miR‐34a, miR‐146a and DNMT3a/DNMT3b along with the gradual decrease in the levels of DNMT1, HDAC1, active marks of chromatin (H4hyperAc, H3K9ac, H3K4me3) and the repressive H3K9me3 mark. Differentiation was accompanied by the down‐regulation of PRC1/2 proteins (BMI1/SUZ12, EZH2) and the retention of the repressive H3K27me3 mark. We report that both AF‐MSCs of normal and fetus‐affected gestations possess differentiation capacity toward myogenic and neural lineages through rather similar epigenetic mechanisms that may provide potential applications for further investigation of the molecular basis of prenatal diseases and for the future autologous therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus‐affected gestations: A focus on myogenic and neural differentiations

Gasiūnienė

Zentelytė

Treigytė

et al. 2019

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Our results suggest that cobalt-manganese ferrite nanoparticles are potentially an effective tool for hyperthermic treatment of dog skin mastocytoma. This approach, in addition to killing tumor cells with heat, does not induce normal cell (ASCs) response 46. The function of proper cells cells in the damaged tissues may also be modified by the transfer of cell receptors, cytoplasmic proteins, and mRNAs from surrounding cells by microvesicles (MVs), which are spherical structures in which a part of the cell cytoplasm enriched for mRNA, miRNA, and functional proteins is encapsulated by cell membrane.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Co0.2Mn0.8Fe2O4 Ferrite Nanoparticles on the C2 Canine Mastocytoma Cell Line and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) Cultured Under a Static Magnetic Field: Possible Implications in the Treatment of Dog Mastocytoma

et al. 2017

View full text Add to dashboard Cite

Cobalt manganese ferrite nanoparticles have application potential in the biomedical field, however there is limited information concerning the biological response. The aim of this work was to investigate the cytotoxic potential of cobalt-manganese ferrite nanoparticles in canine mastocytoma tumor cells (C2) and adipose-derived mesenchymal stromal stem cells (ASCs) cultured under a static magnetic field (MF). In this study, we investigated the viability and proliferation rate of ASC and C2 cells cultured with Co0.2Mn0.8Fe2O4 nanoparticles under 0.5T MF. We observed cells morphology and measured intracellular ROS generation. Thermal observations were used to characterize the thermotrophic cell behavior in different condition and RNA level of heat shock proteins and apoptotic genes was measured. Nanoparticles reduced cell viability, caused cell damage, i.e., through the formation of reactive oxygen species (ROS) and increased transcriptional level of apoptotic genes (Bcl-2, Bax, p53, p21). In addition, we have found that C2 mastocytoma cells cultured with metal oxide nanoparticles under MF exhibited unexpected biological responses, including thermotolerance and apoptotic response induced by the expression of heat shock proteins and ROS produced under a MF. Our results suggest that stimulation using MF and Co0.2Mn0.8Fe2O4 nanoparticles is involved in mechanisms associated with controlling cell proliferative potential signaling events. We can state that significant differences between normal and cancer cells in response to nanoparticles and MF are apparent. Our results show that nanoparticles and MF elevate the temperature in vitro in tumor cells, thereby increasing the expression of ROS as well as heat shock proteins.Electronic supplementary materialThe online version of this article (doi:10.1007/s12195-017-0480-0) contains supplementary material, which is available to authorized users.

show abstract

“…The decreased cellular activity and viability of OECs in the second experimental group might also be explained by the downregulation of the Lin28 transcript, a commonly known regulator of cellular metabolism. As methylation plays a crucial role in directing the fate of cells [Ratajczak et al, 2014], we established the expression of demethylation-related genes. We observed increased genomic methylation in the second experimental group over time.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors

Marycz

Kornicka²,

Grzesiak³

et al. 2017

Cells Tissues Organs

Self Cite

View full text Add to dashboard Cite

The olfactory bulb (OB) is a unique structure in the central nervous system that retains the ability to create new neuronal connections. Glial cells isolated from the OB have been recently considered as a novel and promising tool to establish an effective therapy for central nervous system injuries. Due to the hindered access to autologous tissue for cell isolation, an allogeneic source of tissues obtained postmortem has been proposed. In this study, we focused on the morphological and molecular characteristics of human OB-derived glial cells isolated postmortem, at different time points after a donor's death. We evaluated the proliferative activity of the isolated cells, and investigated the ultrastructure of the mitochondria, the accumulation of intracellular reactive oxygen species, and the activity of superoxide dismutase. The data obtained clearly indicate that the duration of ischemia is crucial for the viability/senescence rate of OB-derived glial cells. The OB can be isolated during autopsy and still stand as a source of viable glial cells, but ischemia duration is a major factor limiting its potential usefulness in therapies.

show abstract

Very small embryonic-like stem cells as a novel developmental concept and the hierarchy of the stem cell compartment

Cited by 44 publications

References 121 publications

Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus‐affected gestations: A focus on myogenic and neural differentiations

Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus‐affected gestations: A focus on myogenic and neural differentiations

The Effect of Co0.2Mn0.8Fe2O4 Ferrite Nanoparticles on the C2 Canine Mastocytoma Cell Line and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) Cultured Under a Static Magnetic Field: Possible Implications in the Treatment of Dog Mastocytoma

The Impact of Oxidative Stress Factors on the Viability, Senescence, and Methylation Status of Olfactory Bulb-Derived Glial Cells Isolated from Human Cadaver Donors

Contact Info

Product

Resources

About