Vesicles in Nature and the Laboratory: Elucidation of Their Biological Properties and Synthesis of Increasingly Complex Synthetic Vesicles

Fernández-Trillo, Francisco; Grover, Liam M.; Stephenson-Brown, Alex; Harrison, Paul; Mendes, Paula M.

doi:10.1002/anie.201607825

Cited by 67 publications

(58 citation statements)

References 216 publications

(414 reference statements)

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“…The use of advanced microscopy techniques makes possible to visualise the surface topology of the most effective nanoscale vector present in nature, the virus and suggests that the presence of a pattern or domain on the NP surface facilitates cellular endocytosis by matching specific targets of the cellular surface. This new understanding is opening new horizons in the development of nanotechnology making it possible to manipulate, control and mimic membrane properties in order to create fully synthetic, nature inspired systems [72,73].…”

Section: Nanoparticle-membrane Interactions: Elastic Theorymentioning

confidence: 99%

Nanoparticle–membrane interactions

Contini

Schneemilch

Gaisford

et al. 2017

Journal of Experimental Nanoscience

154

109

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Engineered nanomaterials have a wide range of applications and as a result, are increasingly present in the environment. While they offer new technological opportunities, there is also the potential for adverse impact, in particular through possible toxicity. In this review, we discuss the current state of the art in the experimental characterisation of nanoparticle-membrane interactions relevant to the prediction of toxicity arising from disruption of biological systems. One key point of discussion is the urgent need for more quantitative studies of nano-bio interactions in experimental models of lipid system that mimic in vivo membranes.

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Section: Nanoparticle-membrane Interactions: Elastic Theorymentioning

confidence: 99%

Nanoparticle–membrane interactions

Contini

Schneemilch

Gaisford

et al. 2017

Journal of Experimental Nanoscience

154

109

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“…EV layers may be used as novel accurate models to investigate properties of biological membranes. Finally, the reported findings also originally contribute to the effort to prepare biosensing platforms that take advantage of biomolecules embedded/supported in membranes [54], circumventing complex time-consuming procedures required to prepare artificial vesicles [55,56].…”

Section: Discussionmentioning

confidence: 87%

Interaction of Extracellular Vesicles with Si Surface Studied by Nanomechanical Microcantilever Sensors

et al. 2018

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Abstract:We report on the interaction of small (<150 nm) extracellular vesicles (EVs) with silicon surface. The study is conducted by leveraging Si nanomechanical microcantilever sensors actuated in static and dynamic modes, that allow tracking of EV collective adsorption energy and adsorbed mass. Upon incubation for 30 min at about 10 nM concentration, EVs isolated from human vascular endothelial cell (HVEC) lines form a patchy layer that partially covers the Si total surface. Formation of this layer releases a surface energy equal to (8 ± 1) mJ/m 2 , typical of weak electrostatic interactions. These findings give a first insight into the EV-Si interface and proof the possibility to realize new hybrid biointerphases that can be exploited as advanced models to investigate properties of biological membranes and/or biosensing platforms that take advantage of biomolecules embedded/supported in membranes.

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“…The laterally attached alkyl chains (linear or branched) were expected to improve the compatibility of the rods with the alkyl chains of the phospholipids, whereas replacing the alkyl chains by semiperfluorinated chains is assumed to modify the interaction with the phospholipids [69,70]. If incorporated into lipid membranes, these X-shaped bolapolyphiles could provide membrane stabilization or destabilization (channel formation) [71], domain formation [72][73][74][75] and membrane compartmentalization [76] and might contribute to the knowledge about the effects of rod-like molecules (e.g., cholesterol) [75] or superstructures (α-helices of integral proteins) [77,78] on the membrane properties. Furthermore, ordered organizations of π-conjugated systems in lipid membranes are of potential interest for artificial light harvesting systems [79] and transmembrane electronic conduction [80].…”

Section: Methodsmentioning

confidence: 99%

Effects of Lateral and Terminal Chains of X-Shaped Bolapolyphiles with Oligo(phenylene ethynylene) Cores on Self-Assembly Behaviour. Part 1: Transition between Amphiphilic and Polyphilic Self-Assembly in the Bulk

Poppe

Ebert

et al. 2017

Polymers

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Polyphilic self-assembly leads to compartmentalization of space and development of complex structures in soft matter on different length scales, reaching from the morphologies of block copolymers to the liquid crystalline (LC) phases of small molecules. Whereas block copolymers are known to form membranes and interact with phospholipid bilayers, liquid crystals have been less investigated in this respect. Here, series of bolapolyphilic X-shaped molecules were synthesized and investigated with respect to the effect of molecular structural parameters on the formation of LC phases (part 1), and on domain formation in phospholipid bilayer membranes (part 2). The investigated bolapolyphiles are based on a rod-like π-conjugated oligo(phenylene ethynylene) (OPE) core with two glycerol groups being either directly attached or separated by additional ethylene oxide (EO) units to both ends. The X-shape is provided by two lateral alkyl chains attached at opposite sides of the OPE core, being either linear, branched, or semiperfluorinated. In this report, the focus is on the transition from polyphilic (triphilic or tetraphilic) to binary amphiphilic self-assembly. Polyphilic self-assembly, i.e., segregation of all three or four incorporated units into separate nano-compartments, leads to the formation of hexagonal columnar LC phases, representing triangular honeycombs. A continuous transition from the well-defined triangular honeycomb structures to simple hexagonal columnar phases, dominated by the arrangement of polar columns on a hexagonal lattice in a mixed continuum formed by the lipophilic chains and the OPE rods, i.e., to amphiphilic self-assembly, was observed by reducing the length and volume of the lateral alkyl chains. A similar transition was found upon increasing the length of the EO units involved in the polar groups. If the lateral alkyl chains are enlarged or replaced by semiperfluorinated chains, then the segregation of lateral chains and rod-like cores is retained, even for enlarged polar groups, i.e., the transition from polyphilic to amphiphilic self-assembly is suppressed.

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Vesicles in Nature and the Laboratory: Elucidation of Their Biological Properties and Synthesis of Increasingly Complex Synthetic Vesicles

Cited by 67 publications

References 216 publications

Nanoparticle–membrane interactions

Nanoparticle–membrane interactions

Interaction of Extracellular Vesicles with Si Surface Studied by Nanomechanical Microcantilever Sensors

Effects of Lateral and Terminal Chains of X-Shaped Bolapolyphiles with Oligo(phenylene ethynylene) Cores on Self-Assembly Behaviour. Part 1: Transition between Amphiphilic and Polyphilic Self-Assembly in the Bulk

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